Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EJ103_RS14345 Genome accession   NZ_CP034439
Coordinates   3189719..3190948 (+) Length   409 a.a.
NCBI ID   WP_126008287.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. Xi13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3184719..3195948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJ103_RS14320 (EJ103_14320) ampE 3184827..3185678 (-) 852 WP_126008279.1 beta-lactamase regulator AmpE -
  EJ103_RS14325 (EJ103_14325) ampD 3185692..3186246 (-) 555 WP_126008281.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EJ103_RS14330 (EJ103_14330) nadC 3186462..3187307 (+) 846 WP_126008283.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EJ103_RS14335 (EJ103_14335) - 3187782..3188219 (+) 438 WP_126008285.1 pilin -
  EJ103_RS14340 (EJ103_14340) - 3188286..3189629 (+) 1344 WP_164521629.1 O-antigen ligase family protein -
  EJ103_RS14345 (EJ103_14345) pilC 3189719..3190948 (+) 1230 WP_126008287.1 type II secretion system F family protein Machinery gene
  EJ103_RS14350 (EJ103_14350) pilD 3191108..3192010 (+) 903 WP_126008289.1 A24 family peptidase Machinery gene
  EJ103_RS14355 (EJ103_14355) coaE 3192012..3192632 (+) 621 WP_126008291.1 dephospho-CoA kinase -
  EJ103_RS14360 (EJ103_14360) pilB 3192779..3194455 (+) 1677 WP_126008292.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EJ103_RS14365 (EJ103_14365) yacG 3194503..3194733 (+) 231 WP_024031401.1 DNA gyrase inhibitor YacG -
  EJ103_RS14370 (EJ103_14370) - 3194800..3195291 (-) 492 WP_004588607.1 Spy/CpxP family protein refolding chaperone -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44288.24 Da        Isoelectric Point: 9.7517

>NTDB_id=331275 EJ103_RS14345 WP_126008287.1 3189719..3190948(+) (pilC) [Pseudoalteromonas sp. Xi13]
MAKKQEDKGLDTFQWVGVSARGKKLEGELTGSSVALVKAQLRKQGITPSKVKRKPKPLFGIQSVQKITPKDIALVTRQIA
TMLMAGVPLIQAIEMIGSGSTNKSVGKLMETIGDEVKAGQPLSQALRKHPRYFDDLYCDLVASGEQSGALDKIFDRVALY
KEKSEALKSKIKKAMFYPISVLVIAGIVTSILLIFVVPQFQDIFAGFGAQLPAFTLFVIGISEFMQEYWWVILIAIVGFG
YAFKEAKLRSLKLRDATDRAILKLPVIGMILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRYAILDIKAEV
SSGNQMNWAMRNSKIFPDMVIQMVAIGEESGSLDGMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLVGGLIVAMYL
PIFQLGSVI

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=331275 EJ103_RS14345 WP_126008287.1 3189719..3190948(+) (pilC) [Pseudoalteromonas sp. Xi13]
ATGGCAAAAAAACAAGAAGATAAAGGCCTTGATACCTTTCAATGGGTAGGCGTAAGTGCACGAGGGAAAAAACTTGAAGG
GGAGCTAACAGGCTCTAGTGTTGCCCTAGTTAAAGCCCAGCTTAGAAAACAAGGTATCACCCCATCAAAAGTTAAGCGCA
AGCCTAAGCCGTTATTTGGCATACAAAGTGTTCAAAAAATTACTCCTAAAGATATTGCGTTAGTTACACGCCAAATTGCC
ACTATGTTAATGGCCGGCGTTCCATTAATTCAAGCCATTGAAATGATTGGCTCAGGTTCCACCAACAAAAGTGTTGGTAA
GTTAATGGAAACCATTGGCGATGAAGTTAAAGCAGGTCAACCTCTCTCACAAGCACTTAGAAAACACCCTCGCTATTTTG
ATGATTTGTATTGTGATTTAGTGGCATCGGGCGAGCAATCGGGTGCGCTTGATAAAATATTTGACCGGGTAGCTTTATAT
AAAGAAAAATCTGAAGCGCTCAAATCAAAAATTAAAAAAGCGATGTTTTACCCTATCTCAGTATTGGTTATTGCTGGCAT
TGTAACCTCTATTTTACTTATATTCGTAGTACCTCAGTTTCAAGATATATTTGCAGGATTTGGCGCACAACTACCTGCCT
TTACCTTATTTGTTATTGGTATATCGGAATTTATGCAGGAATATTGGTGGGTGATTTTAATAGCCATTGTTGGCTTTGGT
TACGCGTTCAAAGAAGCCAAGCTCAGAAGTTTAAAACTTAGAGATGCAACAGACCGGGCTATATTAAAACTCCCTGTAAT
TGGTATGATTTTAAATAAAGCCGCTGTTGCACGATATGCTCGTACGCTTTCGACCACCTTTGCTGCGGGTGTACCCCTTG
TTGATGCGCTTGACTCAGCTGCTGGTGCATCCGGTAATGCTGTGTATCGCTATGCTATTTTAGATATTAAAGCAGAGGTG
AGCTCGGGTAATCAAATGAACTGGGCGATGCGTAACTCTAAAATATTCCCTGATATGGTTATTCAAATGGTAGCCATTGG
TGAAGAGTCTGGCTCTTTAGATGGTATGCTAGCCAAAGTAGCAACAATTTACGAGCAAGAGGTTGATGATGCAGTTGATG
GCTTATCTAGCTTATTAGAGCCGCTTATTATGGCAGTGCTTGGTGTGCTTGTAGGTGGCTTAATTGTTGCCATGTACTTG
CCAATTTTCCAGTTAGGCTCTGTAATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)

Type II secretion system (T2SS), protein F (T2SSF)

(76-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

63.476

97.066

0.616
  pilC Legionella pneumophila strain ERS1305867

56.25

97.8

0.55
  pilC Acinetobacter baumannii D1279779

56

97.8

0.548
  pilC Acinetobacter baylyi ADP1

55

97.8

0.538
  pilC Vibrio cholerae strain A1552

44.776

98.289

0.44
  pilG Neisseria meningitidis 44/76-A

44.643

95.844

0.428
  pilG Neisseria gonorrhoeae MS11

44.388

95.844

0.425
  pilC Vibrio campbellii strain DS40M4

43

97.8

0.421

Multiple sequence alignment