Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EJ103_RS14360 Genome accession   NZ_CP034439
Coordinates   3192779..3194455 (+) Length   558 a.a.
NCBI ID   WP_126008292.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. Xi13     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3187779..3199455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJ103_RS14335 (EJ103_14335) - 3187782..3188219 (+) 438 WP_126008285.1 pilin -
  EJ103_RS14340 (EJ103_14340) - 3188286..3189629 (+) 1344 WP_164521629.1 O-antigen ligase family protein -
  EJ103_RS14345 (EJ103_14345) pilC 3189719..3190948 (+) 1230 WP_126008287.1 type II secretion system F family protein Machinery gene
  EJ103_RS14350 (EJ103_14350) pilD 3191108..3192010 (+) 903 WP_126008289.1 A24 family peptidase Machinery gene
  EJ103_RS14355 (EJ103_14355) coaE 3192012..3192632 (+) 621 WP_126008291.1 dephospho-CoA kinase -
  EJ103_RS14360 (EJ103_14360) pilB 3192779..3194455 (+) 1677 WP_126008292.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EJ103_RS14365 (EJ103_14365) yacG 3194503..3194733 (+) 231 WP_024031401.1 DNA gyrase inhibitor YacG -
  EJ103_RS14370 (EJ103_14370) - 3194800..3195291 (-) 492 WP_004588607.1 Spy/CpxP family protein refolding chaperone -
  EJ103_RS14375 (EJ103_14375) - 3195467..3196156 (+) 690 WP_126008294.1 response regulator -
  EJ103_RS14380 (EJ103_14380) - 3196134..3197549 (+) 1416 WP_039972343.1 ATP-binding protein -
  EJ103_RS14385 (EJ103_14385) - 3197552..3198499 (+) 948 WP_126008296.1 alpha/beta fold hydrolase -
  EJ103_RS14390 (EJ103_14390) - 3198459..3199376 (-) 918 WP_029773365.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61977.14 Da        Isoelectric Point: 5.0828

>NTDB_id=331277 EJ103_RS14360 WP_126008292.1 3192779..3194455(+) (pilB) [Pseudoalteromonas sp. Xi13]
MNINSPLLRKFITLGRIDADTVKAKQQEFASTAELISKCGKIDSNDLAEQCIDLFRVPYFDLKDFDPAQIPEDLVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEIVVVDYIQLDSKIEQLLDAAGSLDLSADEFKEFADLDNEG
PKETTPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVANPPNTLASKLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEEDQKKLYMDALGQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINPKAEMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPETLPAEELARQGFSTTQIEDMTL
YAPKGCENCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLQAGFNNLRLSGLRKAADGLTSLAEINRVTNM

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=331277 EJ103_RS14360 WP_126008292.1 3192779..3194455(+) (pilB) [Pseudoalteromonas sp. Xi13]
ATGAATATTAACTCACCGTTATTGAGAAAATTTATAACGCTTGGCCGCATTGATGCCGATACCGTAAAAGCAAAACAGCA
AGAGTTTGCCTCAACCGCTGAGCTTATATCTAAATGTGGAAAAATTGACAGCAACGACTTAGCTGAACAATGTATAGACT
TATTTCGCGTACCGTATTTTGATTTAAAAGACTTCGACCCTGCACAAATCCCCGAAGACTTAGTTAAAGAAAAGCTAATA
CGTAAACACCACATTTTACCGTTGGTTCAAAAAGGCAGAAAAGTATACATTGCCGCCTCAGATCCGACCGATTACGGTGC
CTTTGAAAACTTTGAATTTAGCACCGGCTTATCGTGCGAAATAGTCGTGGTTGACTATATTCAGCTGGATAGCAAAATTG
AGCAACTACTTGATGCTGCAGGCAGCTTAGATTTAAGCGCCGATGAATTTAAAGAGTTTGCCGACTTAGATAATGAAGGC
CCTAAAGAAACCACACCTAAAGATGATGATAAAGACGATGCGCCAATCATTGTTTATATCAATAAAATATTAATGGATGC
CATTAAAAAAGGCGCTTCTGATTTACACTTTGAACCTTACGAGCACAAATACCGTATTCGCTTTCGTATTGATGGCATTT
TGCATGAAGTTGCCAACCCTCCCAATACCTTAGCAAGTAAACTGTCGGCGCGTATAAAAGTAATGTCGAGGCTCGATATT
GCTGAAAAACGTAAACCACAAGATGGCCGTATTAAATTAAAAATTACCGAGCGTAAAAGCATCGATTTTCGTGTAAGTAC
TATGCCTACGTTATGGGGCGAAAAAATAGTAATGCGTATTCTTGATTCATCAAGCGCTATGCTAGGTATTGACGTATTAG
GTTACGAAGAGGATCAAAAAAAGCTCTACATGGATGCGCTTGGGCAACCACAAGGCATGATACTAGTGACCGGCCCAACG
GGCTCTGGTAAAACCGTATCGCTTTATACAGGTTTAAATATACTTAACCAGCCGGAGCGTAACATTAGTACCGCCGAGGA
TCCGGTTGAGATAAACCTTGAAGGGGTTAACCAAGTACAAATTAACCCGAAAGCCGAAATGACCTTTGCTAACGCCTTGC
GTGCATTTTTACGTCAAGATCCAGATGTAGTGATGGTAGGTGAAATACGTGACCTAGAAACCGCAGAAATTTCAATTAAA
GCGGCGCAAACAGGTCACTTAGTACTATCAACACTACATACCAACTCAGCGCCCGAAACCATAACGCGTTTATTAAATAT
GGGCGTGCCTGCGTATAACGTGGCAAGCTCTATAAGTTTGATTATTGCCCAGCGCTTAGCTAGGCGTTTATGCCCCAAGT
GTAAAACCCCAGAAACACTGCCAGCAGAAGAGTTGGCTCGTCAAGGTTTTAGCACAACACAAATTGAAGATATGACACTA
TACGCACCCAAAGGCTGCGAAAATTGCACCGATGGTTACAAAGGCCGTGTGGGTATTTACGAAGTAATGCAAATTACTCC
TGAAATTGCACAAATTATTATGCGCGGTGGCAACTCGCTCGAAATCGCAGAGGTATCGCTACAAGCTGGGTTCAATAACT
TGCGATTATCGGGTTTACGAAAAGCCGCCGATGGTTTAACGTCTCTGGCTGAAATTAATCGCGTAACCAATATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.81

100

0.568
  pilB Legionella pneumophila strain ERS1305867

53.165

99.104

0.527
  pilB Acinetobacter baylyi ADP1

58.02

90.502

0.525
  pilB Vibrio parahaemolyticus RIMD 2210633

53.497

94.803

0.507
  pilB Vibrio cholerae strain A1552

52.239

96.057

0.502
  pilB Vibrio campbellii strain DS40M4

51.88

95.341

0.495
  pilF Neisseria gonorrhoeae MS11

49.042

93.548

0.459

Multiple sequence alignment