Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   EH233_RS06110 Genome accession   NZ_CP034058
Coordinates   1424451..1426463 (+) Length   670 a.a.
NCBI ID   WP_011317260.1    Uniprot ID   -
Organism   Anabaena sp. YBS01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1419451..1431463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EH233_RS06100 (EH233_06085) dnaK 1421060..1423021 (-) 1962 WP_011317262.1 molecular chaperone DnaK -
  EH233_RS06105 (EH233_06090) grpE 1423297..1424043 (-) 747 WP_011317261.1 nucleotide exchange factor GrpE -
  EH233_RS06110 (EH233_06095) pilB/pilB1 1424451..1426463 (+) 2013 WP_011317260.1 GspE/PulE family protein Machinery gene
  EH233_RS06115 (EH233_06100) pilT 1426511..1427629 (+) 1119 WP_011317259.1 type IV pilus twitching motility protein PilT Machinery gene
  EH233_RS06120 (EH233_06105) - 1427731..1428954 (+) 1224 WP_011317258.1 type II secretion system F family protein -
  EH233_RS06125 (EH233_06110) - 1429000..1429554 (+) 555 WP_011317257.1 hypothetical protein -
  EH233_RS06130 (EH233_06115) - 1429622..1430626 (-) 1005 WP_011317256.1 hypothetical protein -
  EH233_RS06135 - 1430684..1430884 (+) 201 WP_153228360.1 hypothetical protein -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74978.89 Da        Isoelectric Point: 5.1833

>NTDB_id=328630 EH233_RS06110 WP_011317260.1 1424451..1426463(+) (pilB/pilB1) [Anabaena sp. YBS01]
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGYINTEQMRQALIESRKSGRPLTEVLESITGRQLSPEYLRQYKKQQLFELK
ILYGVEYLDPEVNDIGSTMVGNLIETLIPVDICRRHRLVPLSKNDEHTPPYVLVAMVAPDNLEASDDLNRILRPQGLTLQ
RMVITQEDYQRLINQYLDELAVRQKHLEQEKFTDINQDLENLGNLDLEDAPEEMEADLGSAMKGAEDAPVINLVNRILAK
ALHDKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLQIVQDMVSKPFGLILVTGPTGSGKTTSLYSALAEKNSPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCGECRIAYTPTTEELARYGMTASQEVSVTFYKANTIPAEAIAEAKAKNQLCPACNG
VGYKGRCGVYEVMRVTENLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAKR
KTGLTCRTCSAILQPEWLDCPYCLTARFED

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=328630 EH233_RS06110 WP_011317260.1 1424451..1426463(+) (pilB/pilB1) [Anabaena sp. YBS01]
ATGACTTACTCGTCACCACAACGGCGCAGTACCGCTCTTACTACAAGAACAGAGTTTTCACCTTTCGGTAATAAGCTAGT
GCAGTCTGGCTACATTAACACCGAACAGATGAGACAGGCGCTGATTGAAAGTCGCAAGTCTGGTAGACCCTTAACAGAAG
TTTTGGAGTCAATTACTGGGCGACAATTATCACCTGAGTACCTCAGGCAGTACAAGAAACAACAATTATTTGAACTGAAA
ATACTATACGGTGTTGAATACCTCGATCCGGAGGTCAACGATATCGGTAGCACGATGGTGGGTAATTTAATTGAAACCCT
CATCCCAGTTGATATCTGCCGTCGTCATCGTTTAGTACCACTATCTAAAAATGATGAACACACCCCGCCTTATGTTTTAG
TGGCGATGGTTGCTCCGGATAACTTGGAAGCGTCGGACGACCTAAACCGCATCTTGCGCCCCCAAGGTTTAACATTACAG
CGTATGGTAATTACCCAGGAGGATTACCAACGGCTAATTAACCAATATTTGGATGAATTGGCTGTGCGGCAAAAGCACCT
GGAACAAGAAAAATTTACAGATATTAATCAGGATTTAGAAAATCTTGGCAATCTCGACCTAGAAGATGCTCCTGAAGAGA
TGGAGGCTGATTTAGGTTCAGCGATGAAGGGTGCAGAAGATGCACCTGTCATCAACCTCGTTAACAGAATTTTAGCGAAG
GCGTTGCATGACAAGGTTTCTGATATCCACATTGAACCCCAAGAAGAAAATCTGCGGATTCGCTTCCGCAAGGATGGGGT
ACTGCGGGAGGCTTTTGACCCCTTACCGAAAAAAATTATTCCGGCTGTAACAGCCAGATTTAAAATTATCTCCAATTTGG
ATATTGCCGAGCGACGTTTACCGCAAGATGGACGCATCCGCCGGATGTTTGAGGGACGTAAAGTAGACTTCCGCGTGAAT
ACCTTACCCAGTCGCTATGGGGAAAAGGTAGTACTGCGGATTTTGGACAACTCTTCTACGCAACTGGGACTGAATAAGTT
AATTACTGACCCAGAAACATTGCAGATTGTCCAAGATATGGTGAGTAAGCCTTTCGGTTTAATTTTGGTAACTGGGCCAA
CTGGTTCAGGTAAGACAACTTCCTTATATTCAGCACTGGCGGAGAAGAACTCACCAGGTATTAATATCAGTACGGTAGAA
GACCCAATTGAGTACAGTTTGCCAGGGATTACCCAGGTACAGGTAATTAGAGAAAAAGGGCTAGATTTTGCGACGGCATT
ACGGGCTTTTTTGCGACAAGACCCCGATGTGTTGCTGGTGGGTGAAACAAGAGACAAAGAAACAGCGAAAACAGCGATTG
AAGCGGCTTTAACAGGTCACTTGGTATTAACTACATTACACACCAACGATGCCCCTGGTGCGATCGCTCGTTTGGGTGAA
ATGGGTATTGAACCATTCATGGTTTCCAGTTCTCTGATTGGGGTATTGGCACAACGTTTAGTGCGCCGTGTTTGTGGTGA
GTGCCGTATTGCTTACACTCCCACCACTGAGGAGTTAGCTCGTTATGGCATGACGGCTTCTCAAGAAGTGTCTGTCACCT
TCTATAAAGCTAACACCATCCCCGCAGAAGCGATCGCCGAGGCCAAAGCGAAAAACCAGCTTTGTCCAGCTTGTAACGGT
GTTGGCTACAAAGGACGCTGTGGTGTTTATGAAGTGATGCGGGTTACAGAGAACCTACAAACTTTAATCAACGAAGAAGC
ACCAACAGAACGCATCAAAGAAGTAGCTGTAGAAGAAGGGATGAAAACCTTATTAGCTTATAGCTTAGACCTCGTTCGCC
AAGGTGCTACCACCCTAGAAGAAGTGGAACGGGTGACATTTACAGACACAGGTTTAGAAGCTGAATTAAAAGCTAAACGC
AAGACTGGTCTTACCTGCCGGACTTGCAGCGCCATATTACAACCAGAATGGCTAGATTGTCCCTACTGTCTAACAGCCCG
TTTTGAAGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-179)

Type II/IV secretion system protein (T2SSE)

(236-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.881

100

0.651
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.11

94.776

0.361

Multiple sequence alignment