Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EEA47_RS06220 Genome accession   NZ_CP033457
Coordinates   1324380..1325603 (+) Length   407 a.a.
NCBI ID   WP_149192422.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain SNU_SP1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1319380..1330603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EEA47_RS06200 (EEA47_06420) ampD 1320392..1320943 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EEA47_RS06205 (EEA47_06425) nadC 1321036..1321923 (+) 888 WP_005386616.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EEA47_RS06210 (EEA47_06430) - 1322186..1322671 (+) 486 WP_005386619.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EEA47_RS06215 (EEA47_06435) pilB 1322671..1324356 (+) 1686 WP_268207500.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EEA47_RS06220 (EEA47_06440) pilC 1324380..1325603 (+) 1224 WP_149192422.1 type II secretion system F family protein Machinery gene
  EEA47_RS06225 (EEA47_06445) pilD 1325668..1326537 (+) 870 WP_012841418.1 prepilin peptidase Machinery gene
  EEA47_RS06230 (EEA47_06450) coaE 1326538..1327152 (+) 615 WP_017635920.1 dephospho-CoA kinase -
  EEA47_RS06235 (EEA47_06455) zapD 1327180..1327920 (+) 741 WP_005373086.1 cell division protein ZapD -
  EEA47_RS06240 (EEA47_06460) yacG 1328079..1328273 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  EEA47_RS06245 (EEA47_06465) rplS 1329253..1329606 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  EEA47_RS06250 (EEA47_06470) trmD 1329648..1330409 (-) 762 WP_005385420.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45282.43 Da        Isoelectric Point: 10.4523

>NTDB_id=323793 EEA47_RS06220 WP_149192422.1 1324380..1325603(+) (pilC) [Vibrio alginolyticus strain SNU_SP1]
MKSTTPQLKNFRWKGINSSGKKTSGQTLAMTEIEVRERLNAQHIKIKKLKKSSISFLTKLSHRVKGRDITVFTRQISTML
ITGVPLVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASEHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVVLVALGVSFIMLTKVIPEFEKMFVGFGADLPWFTRQVLDLSAWTQNWSPFIALGSISLFISA
RILSKRSDSFRLTLNRSVLKFPVLGPVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNLHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=323793 EEA47_RS06220 WP_149192422.1 1324380..1325603(+) (pilC) [Vibrio alginolyticus strain SNU_SP1]
ATGAAAAGCACTACACCACAACTTAAAAACTTCCGCTGGAAAGGCATCAATAGTTCAGGCAAAAAGACGTCTGGACAAAC
CCTCGCGATGACAGAAATTGAAGTACGCGAACGTCTAAACGCACAGCATATCAAGATCAAGAAGTTAAAGAAAAGCAGTA
TCTCGTTTCTCACTAAACTCAGCCATCGCGTAAAAGGCAGAGACATCACGGTGTTTACCCGTCAAATTTCGACGATGTTG
ATAACCGGTGTGCCCTTAGTTCAGGCTTTAAAATTGGTATCAGAAAATCACAAAAAAGCAGAGATGAAATCCATTTTGAT
GAGCGTAACACGCGCCGTCGAAGCGGGTACGCCTATGTCAAAAGCCATGCGCACTGCAAGTGAACACTTTGACCCACTCT
ATACCGACCTTATCGCAACAGGTGAGCAATCCGGTAACTTAGCGGAAGTGTTCGAGCGCTTAGCCACCTACCGAGAAAAA
AATGAACAACTCCGTGCGAAAGTGATCAAAGCACTGATCTACCCAGCGATGGTAGTACTAGTCGCGTTAGGCGTGTCGTT
TATCATGCTCACCAAAGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGTTTTGGTGCTGACTTACCTTGGTTTACCAGGC
AAGTATTAGATCTTTCCGCCTGGACACAAAACTGGAGTCCGTTTATCGCACTAGGCTCCATTAGTTTATTCATCTCGGCG
AGAATCCTCTCTAAGCGTTCAGATTCTTTTCGCTTAACGCTCAATCGATCTGTGCTTAAATTTCCTGTCCTTGGGCCTGT
ATTATCAAAAGCAGCGATCGCCAAATTTAGTCGAACACTCGCCACAAGCTTTACAGCGGGCATTCCAATTCTAACCAGTT
TAAAAACTACATCAAAAACATCAGGAAACTTGCACTATCAACTCGCCATAGAAGAAGTTTACCGTGATACCGCCGCAGGT
ATGCCAATGTATGTAGCTATGCGCAACTGTAATGTGTTCCCTGAATTGGTGTTACAAATGGTTATGATCGGTGAAGAGTC
TGGTCGACTTGACGATATGCTCAATAAAGTTGCAACAATCTACGAGTTTGAAGTCGACAACACCGTCGACAACCTTAGTA
AAATTCTAGAGCCACTAATTATCGTTTTTTTAGGTATCGTTGTTGGCGGATTAGTGACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

86.978

100

0.87
  pilC Vibrio cholerae strain A1552

73.775

100

0.74
  pilC Acinetobacter baylyi ADP1

43

98.28

0.423
  pilC Acinetobacter baumannii D1279779

41.439

99.017

0.41
  pilC Legionella pneumophila strain ERS1305867

40.541

100

0.405
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilG Neisseria meningitidis 44/76-A

40.247

99.509

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398

Multiple sequence alignment