Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EEA47_RS06215 Genome accession   NZ_CP033457
Coordinates   1322671..1324356 (+) Length   561 a.a.
NCBI ID   WP_268207500.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain SNU_SP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1317671..1329356
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EEA47_RS06195 (EEA47_06415) pdhR 1319219..1319986 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  EEA47_RS06200 (EEA47_06420) ampD 1320392..1320943 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EEA47_RS06205 (EEA47_06425) nadC 1321036..1321923 (+) 888 WP_005386616.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EEA47_RS06210 (EEA47_06430) - 1322186..1322671 (+) 486 WP_005386619.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EEA47_RS06215 (EEA47_06435) pilB 1322671..1324356 (+) 1686 WP_268207500.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EEA47_RS06220 (EEA47_06440) pilC 1324380..1325603 (+) 1224 WP_149192422.1 type II secretion system F family protein Machinery gene
  EEA47_RS06225 (EEA47_06445) pilD 1325668..1326537 (+) 870 WP_012841418.1 prepilin peptidase Machinery gene
  EEA47_RS06230 (EEA47_06450) coaE 1326538..1327152 (+) 615 WP_017635920.1 dephospho-CoA kinase -
  EEA47_RS06235 (EEA47_06455) zapD 1327180..1327920 (+) 741 WP_005373086.1 cell division protein ZapD -
  EEA47_RS06240 (EEA47_06460) yacG 1328079..1328273 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62117.01 Da        Isoelectric Point: 5.1968

>NTDB_id=323792 EEA47_RS06215 WP_268207500.1 1322671..1324356(+) (pilB) [Vibrio alginolyticus strain SNU_SP1]
MLTNLSTVLRQAGLLTFSQEESLLEQVKASGISMPEALLSSGLFTSSELAEHLSSLFGLNQPELSQYEYASLCQQLGLRE
LITRHNALPLQRTPSTLLLAVADPTNQQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRALGQEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRMGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=323792 EEA47_RS06215 WP_268207500.1 1322671..1324356(+) (pilB) [Vibrio alginolyticus strain SNU_SP1]
ATGCTAACCAATCTCTCCACGGTTCTTCGCCAGGCCGGATTACTAACATTTTCCCAAGAAGAATCGTTGCTTGAGCAAGT
CAAAGCTTCTGGCATTTCGATGCCAGAAGCTTTACTCAGTTCAGGGTTATTCACGTCAAGCGAACTCGCTGAGCACTTAA
GCTCTCTCTTTGGTTTAAATCAGCCCGAGTTATCTCAGTACGAGTACGCTTCGCTTTGTCAACAACTCGGTCTACGTGAA
TTAATCACTCGGCATAACGCACTCCCACTCCAACGTACGCCTTCAACGTTATTATTAGCGGTTGCCGACCCCACGAACCA
ACAAGCAGAAGATGACTTCCGCTTTGCGACAGGGTTGCAAGTAGAACTTGTTCTCGCTGATTTTCGCGAGCTGACCGCTG
CGATTCGGCGCTTATATGGCCGCGCACTTGGGCAGGAAAAGTCTGGGTTAAAAGAGATCAACCAAGAAGAGTTAGCCAAC
CTGGTTGATGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGACGAGTCACCCGTCAGCCGTTACATAAA
CCAAATTCTGCTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAGCCTTATGAAAAGATGTACCGCGTTCGCT
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATAAAGCTCAAGTTGAATCAAGACACCGCGAT
TGATATGCGTGTATCCACACTACCGACTCTATTCGGAGAGAAAATTGTATTACGACTGCTCGACAGCAGCTCTGCGTCAC
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTCCGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCAACTGGGAGCGGGAAAACCGTTTCTCTGTACACTGGGCTGAGTATTCTCAATAAACCAGAAATCAA
CATTTCCACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTCTTACGTCAAGACCCCGATGTAGTAATGGTCGGTGAGATTCGTGATTTAGATACC
GCCGAAATCGCTATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTGCATACCAATTCTGCGGCTGAAACGGT
GATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAGTTTGATTATTGCCCAGCGCTTGGCCC
GTAAACTGTGCTCGCACTGTAAACAATCACAAGAGCTTACGGTTCAGCTACAACATCTTGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGCAATGAGTGTACCCATGGTTATTCCGGCCGGATGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGTGCCTCGGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGTGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

95.009

100

0.95
  pilB Vibrio campbellii strain DS40M4

91.087

100

0.911
  pilB Vibrio cholerae strain A1552

74.555

100

0.747
  pilB Acinetobacter baumannii D1279779

51.619

93.583

0.483
  pilB Legionella pneumophila strain ERS1305867

48.387

99.465

0.481
  pilB Acinetobacter baylyi ADP1

47.756

99.287

0.474
  pilF Neisseria gonorrhoeae MS11

44.326

100

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

38.047

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.037

100

0.374
  pilF Thermus thermophilus HB27

36.655

100

0.367

Multiple sequence alignment