Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   JL719_RS02445 Genome accession   NZ_CP033381
Coordinates   548010..549227 (+) Length   405 a.a.
NCBI ID   WP_033155842.1    Uniprot ID   -
Organism   Methylomonas sp. LW13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 543010..554227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JL719_RS02430 (U737_02470) - 543482..543886 (-) 405 WP_033155839.1 hypothetical protein -
  JL719_RS02435 (U737_02475) thiC 544222..546090 (+) 1869 WP_033155840.1 phosphomethylpyrimidine synthase ThiC -
  JL719_RS02440 (U737_02480) pilB 546292..548007 (+) 1716 WP_033155841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JL719_RS02445 (U737_02485) pilC 548010..549227 (+) 1218 WP_033155842.1 type II secretion system F family protein Machinery gene
  JL719_RS02450 (U737_02490) pilD 549301..550167 (+) 867 WP_033155843.1 prepilin peptidase Machinery gene
  JL719_RS02455 (U737_02495) coaE 550171..550782 (+) 612 WP_033155844.1 dephospho-CoA kinase -
  JL719_RS02460 (U737_02500) zapD 550779..551552 (+) 774 WP_051669750.1 cell division protein ZapD -
  JL719_RS02465 (U737_02505) yacG 551552..551755 (+) 204 WP_033155846.1 DNA gyrase inhibitor YacG -
  JL719_RS02470 (U737_02510) - 551772..552716 (-) 945 WP_033155847.1 Nudix family hydrolase -
  JL719_RS02475 (U737_02515) argJ 552729..553943 (-) 1215 WP_033155848.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44403.30 Da        Isoelectric Point: 5.8843

>NTDB_id=323457 JL719_RS02445 WP_033155842.1 548010..549227(+) (pilC) [Methylomonas sp. LW13]
MAKQDEQIEFNWDGFDRQNKKTKGTISAKSEVIARTELRRMGIRVIGIKAKSKPLFGARVQKITPGDIAVFARQLATMLE
AGVPLVQSFDIIGKGHDNASMSEMLLSIKADVEGGDTLAQALNRKPLYFDALFCNLVEAGEHAGVLETLLDKIATYKEKT
ESIKKKVKKALTYPIAVLVVAFIVTAILLIFVVPVFEDLFKGFGADLPAFTQFVIELSAWVQEWWWAVIGVIAITAYVFN
YFKKRSKAFNHFLDKTLLKIPVVGMILEKSAIARFARTLSTMSAAGVPLVEALVSVAGACGNILFYEAVMAVRDDVSTGQ
QLKFALETTGMFPNMVVQMVAIGEESGSIDAMLDKVADFYEEEVDNLVDNLSSLMEPIIMVILGILVGGLIVAMYLPIFK
LGAAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=323457 JL719_RS02445 WP_033155842.1 548010..549227(+) (pilC) [Methylomonas sp. LW13]
ATGGCAAAGCAAGACGAACAAATCGAGTTCAATTGGGACGGTTTCGATAGGCAAAACAAGAAAACCAAAGGCACCATCAG
CGCCAAGAGCGAGGTCATTGCGCGCACCGAATTAAGGCGGATGGGCATTCGGGTCATCGGTATCAAAGCCAAGTCCAAGC
CCTTGTTCGGTGCCAGAGTGCAGAAAATCACGCCCGGCGACATCGCGGTGTTCGCCAGGCAGTTGGCGACTATGCTGGAG
GCCGGGGTGCCGTTGGTGCAGTCATTCGACATTATCGGCAAGGGGCACGACAACGCAAGCATGTCGGAAATGCTGTTATC
GATAAAGGCCGACGTGGAGGGCGGCGACACCTTGGCGCAGGCCTTGAACCGTAAGCCACTGTATTTTGATGCGCTGTTCT
GCAATTTGGTCGAAGCCGGCGAACATGCCGGGGTATTGGAAACTTTGCTCGACAAGATTGCCACCTACAAAGAAAAAACC
GAATCGATCAAAAAGAAGGTTAAAAAAGCATTGACCTACCCGATTGCAGTGTTGGTCGTGGCGTTTATCGTCACCGCGAT
TTTGCTGATTTTCGTGGTGCCGGTGTTTGAGGATTTGTTTAAAGGCTTTGGCGCCGACCTACCGGCATTTACCCAATTTG
TTATTGAGCTGTCGGCGTGGGTGCAGGAATGGTGGTGGGCGGTGATTGGCGTGATCGCCATTACCGCTTACGTGTTCAAT
TATTTTAAAAAGCGCTCGAAGGCGTTTAACCATTTTCTCGATAAAACTTTGCTGAAAATCCCGGTTGTCGGGATGATTCT
GGAAAAATCGGCCATCGCTCGTTTTGCCAGAACACTGTCCACCATGTCTGCGGCTGGCGTACCCTTGGTTGAAGCACTGG
TTTCGGTGGCAGGGGCTTGCGGCAATATTTTGTTCTACGAAGCGGTAATGGCTGTGCGCGACGACGTTTCCACCGGCCAG
CAATTGAAATTTGCCTTGGAGACCACCGGCATGTTTCCGAATATGGTGGTACAAATGGTGGCGATCGGCGAGGAGTCCGG
TTCCATTGATGCGATGCTGGATAAAGTCGCGGACTTTTACGAAGAAGAGGTCGATAACCTGGTCGATAACCTGAGCAGTT
TGATGGAGCCTATCATCATGGTGATCCTGGGCATTTTGGTCGGCGGTTTGATCGTCGCGATGTATCTGCCTATCTTCAAG
CTGGGCGCGGCTATTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

59.296

98.272

0.583
  pilC Pseudomonas stutzeri DSM 10701

57.323

97.778

0.56
  pilC Acinetobacter baumannii D1279779

54.408

98.025

0.533
  pilC Acinetobacter baylyi ADP1

52.696

100

0.531
  pilG Neisseria gonorrhoeae MS11

47.692

96.296

0.459
  pilG Neisseria meningitidis 44/76-A

47.692

96.296

0.459
  pilC Vibrio cholerae strain A1552

42.748

97.037

0.415
  pilC Vibrio campbellii strain DS40M4

40.863

97.284

0.398

Multiple sequence alignment