Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JL719_RS02440 Genome accession   NZ_CP033381
Coordinates   546292..548007 (+) Length   571 a.a.
NCBI ID   WP_033155841.1    Uniprot ID   A0A2N0VDV9
Organism   Methylomonas sp. LW13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541292..553007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JL719_RS02415 (U737_02455) rpmA 541680..541937 (-) 258 WP_020484554.1 50S ribosomal protein L27 -
  JL719_RS02420 (U737_02460) rplU 541955..542266 (-) 312 WP_033156113.1 50S ribosomal protein L21 -
  JL719_RS02425 (U737_02465) - 542473..543477 (+) 1005 WP_051669748.1 polyprenyl synthetase family protein -
  JL719_RS02430 (U737_02470) - 543482..543886 (-) 405 WP_033155839.1 hypothetical protein -
  JL719_RS02435 (U737_02475) thiC 544222..546090 (+) 1869 WP_033155840.1 phosphomethylpyrimidine synthase ThiC -
  JL719_RS02440 (U737_02480) pilB 546292..548007 (+) 1716 WP_033155841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JL719_RS02445 (U737_02485) pilC 548010..549227 (+) 1218 WP_033155842.1 type II secretion system F family protein Machinery gene
  JL719_RS02450 (U737_02490) pilD 549301..550167 (+) 867 WP_033155843.1 prepilin peptidase Machinery gene
  JL719_RS02455 (U737_02495) coaE 550171..550782 (+) 612 WP_033155844.1 dephospho-CoA kinase -
  JL719_RS02460 (U737_02500) zapD 550779..551552 (+) 774 WP_051669750.1 cell division protein ZapD -
  JL719_RS02465 (U737_02505) yacG 551552..551755 (+) 204 WP_033155846.1 DNA gyrase inhibitor YacG -
  JL719_RS02470 (U737_02510) - 551772..552716 (-) 945 WP_033155847.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63210.85 Da        Isoelectric Point: 6.1194

>NTDB_id=323456 JL719_RS02440 WP_033155841.1 546292..548007(+) (pilB) [Methylomonas sp. LW13]
MATAPSNIHFSGLVKCLIQKGLLTEESAATCLQEAQKRKQAFISYLVEHKYADSKTVATLASAEFGVPFFDLDAIDPSFL
PIKLVSEKLIRQHHALPLFKRGNRLFVATADPTNFQALDEIKFHTRHNTENILVEEDKLIRMIDTALEAADTSMKDLLDD
DLDNIDISAGDDSKPADEAIGSDIDDAPIVRFVNKILLDSIKRGVSDIHLEPFEKKFRIRFRADGILSEVASPPVGIANR
IISRLKVMSKMDIAERRVPQDGRIKMQISKNRAIDFRVNTCPTLFGEKVVMRILDPTSAQIGIEKLGFEPEQQKNFLEAI
HKPYGMVLVTGPTGSGKTVSLYTGLNILNSIETNISTAEDPVEITVEGINQVNVNPKAGLTFAEALRAFLRQDPDIIMVG
EIRDLETASIAVKAAQTGHMVLSTLHTNDAPQTINRLVQMGIEPFNIVSAVILIMAQRLARRLCEHCKQEVDYPESTLLS
AGFKQEEIGTFKVYGPVGCEHCNNGYKGRVGIYQVMPITEKMRALILNGGNSMQMAELSKSEGINDLRMSGLLKIKQGVT
SLQEIDRVTKE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=323456 JL719_RS02440 WP_033155841.1 546292..548007(+) (pilB) [Methylomonas sp. LW13]
ATGGCGACAGCACCCTCTAACATCCATTTCAGCGGCTTGGTCAAATGCCTGATTCAGAAAGGTTTGTTGACCGAGGAATC
GGCGGCGACCTGTTTGCAGGAGGCGCAGAAGCGTAAACAAGCGTTTATCAGCTATTTGGTGGAACATAAGTATGCGGACA
GCAAAACCGTGGCTACGCTGGCTTCGGCGGAGTTTGGTGTGCCGTTTTTCGATCTGGATGCCATAGACCCTTCATTCTTG
CCGATCAAACTAGTCAGCGAGAAATTGATTCGCCAGCATCATGCCTTACCTTTATTCAAGCGCGGCAACCGCTTGTTTGT
CGCCACCGCCGATCCGACCAATTTTCAGGCGCTGGACGAGATCAAGTTTCATACCCGGCACAATACCGAAAATATTCTGG
TTGAAGAAGACAAGCTGATCCGCATGATAGACACGGCGCTGGAGGCTGCCGATACGTCGATGAAGGATCTGTTGGACGAC
GATCTGGACAATATCGATATTTCCGCAGGCGACGACAGCAAACCGGCCGACGAAGCAATAGGCTCGGATATTGATGACGC
GCCTATCGTCCGTTTTGTGAACAAAATCCTGCTGGACTCGATCAAACGCGGCGTTTCGGATATACACCTGGAGCCTTTCG
AGAAAAAGTTTCGTATCCGTTTTCGGGCCGACGGTATTTTATCGGAGGTGGCTAGTCCGCCGGTGGGCATTGCCAACCGG
ATTATTTCTCGGCTGAAAGTCATGTCGAAAATGGACATTGCCGAACGGCGGGTGCCGCAGGACGGCCGGATCAAAATGCA
GATTTCCAAGAACCGGGCCATCGATTTTCGGGTAAACACCTGCCCGACGTTGTTCGGTGAAAAAGTGGTAATGCGGATTC
TCGATCCGACCAGTGCGCAAATTGGTATCGAGAAACTCGGCTTCGAACCCGAGCAGCAGAAAAATTTTCTGGAGGCGATC
CACAAGCCTTACGGTATGGTGCTGGTTACCGGGCCGACCGGTAGCGGTAAAACCGTGTCGCTGTATACCGGGCTGAATAT
TCTGAACAGCATCGAAACCAATATTTCCACCGCCGAAGATCCCGTAGAGATCACCGTGGAAGGCATCAACCAAGTCAATG
TCAATCCCAAGGCCGGCCTGACCTTTGCCGAAGCGCTACGGGCGTTTTTGCGGCAAGACCCGGACATCATCATGGTTGGC
GAGATTCGCGACCTGGAAACCGCCAGTATCGCGGTCAAGGCGGCGCAAACCGGTCACATGGTGTTGTCTACCTTGCATAC
CAATGATGCGCCGCAAACCATCAACCGTTTGGTGCAAATGGGCATAGAACCGTTCAATATTGTTTCGGCGGTGATTTTGA
TCATGGCGCAACGGCTGGCGCGGCGCTTATGCGAGCATTGTAAGCAAGAAGTTGATTATCCGGAAAGCACCTTGCTGTCG
GCCGGGTTTAAGCAGGAGGAAATCGGTACGTTTAAAGTCTACGGTCCGGTGGGTTGCGAACATTGCAATAACGGTTACAA
GGGCAGGGTAGGGATCTACCAGGTAATGCCTATCACCGAAAAAATGCGTGCGCTGATTTTGAACGGCGGTAATTCCATGC
AAATGGCGGAATTGTCGAAAAGTGAAGGAATCAACGATTTAAGAATGTCGGGGCTGTTAAAAATTAAGCAAGGCGTCACC
TCGCTGCAAGAAATTGACCGGGTCACCAAGGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-148)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N0VDV9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.323

100

0.555
  pilB Acinetobacter baumannii D1279779

53.403

100

0.536
  pilB Acinetobacter baylyi ADP1

53.014

98.774

0.524
  pilF Neisseria gonorrhoeae MS11

48.387

97.723

0.473
  pilB Vibrio parahaemolyticus RIMD 2210633

46.263

98.424

0.455
  pilB Vibrio campbellii strain DS40M4

45.471

98.599

0.448
  pilB Vibrio cholerae strain A1552

49.606

88.967

0.441
  pilF Thermus thermophilus HB27

39.748

97.373

0.387
  pilB/pilB1 Synechocystis sp. PCC 6803

39.526

96.147

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.64

97.898

0.378

Multiple sequence alignment