Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JL719_RS02450 Genome accession   NZ_CP033381
Coordinates   549301..550167 (+) Length   288 a.a.
NCBI ID   WP_033155843.1    Uniprot ID   -
Organism   Methylomonas sp. LW13     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 544301..555167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JL719_RS02440 (U737_02480) pilB 546292..548007 (+) 1716 WP_033155841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JL719_RS02445 (U737_02485) pilC 548010..549227 (+) 1218 WP_033155842.1 type II secretion system F family protein Machinery gene
  JL719_RS02450 (U737_02490) pilD 549301..550167 (+) 867 WP_033155843.1 prepilin peptidase Machinery gene
  JL719_RS02455 (U737_02495) coaE 550171..550782 (+) 612 WP_033155844.1 dephospho-CoA kinase -
  JL719_RS02460 (U737_02500) zapD 550779..551552 (+) 774 WP_051669750.1 cell division protein ZapD -
  JL719_RS02465 (U737_02505) yacG 551552..551755 (+) 204 WP_033155846.1 DNA gyrase inhibitor YacG -
  JL719_RS02470 (U737_02510) - 551772..552716 (-) 945 WP_033155847.1 Nudix family hydrolase -
  JL719_RS02475 (U737_02515) argJ 552729..553943 (-) 1215 WP_033155848.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 31818.02 Da        Isoelectric Point: 6.9888

>NTDB_id=323458 JL719_RS02450 WP_033155843.1 549301..550167(+) (pilD) [Methylomonas sp. LW13]
MVFATLQHYPLFLISVVCVLGLLVGSFLNVVIYRLPVMMQTGWRRECREYLQLPQDEASEVFNLMLPASHCPSCKTEIKA
YQNIPVISYLLLGGKCAHCGVKIAIRYPLIEAFTGLCSAVVAWHFGYGWALAFALPLTWCLIALSFIDIDQQLLPDSITL
PMLWLGLLLSLFDIYTNSHDAIIGAVAGYLSLWSVYQLFKLLTGKEGMGYGDFKLLALFGAWLGWQYLPLIILLSSLVGA
VIGIAMIVFGKRDAGKPIPFGPYLAMAGWLAMLFGPELNALYLHSAGL

Nucleotide


Download         Length: 867 bp        

>NTDB_id=323458 JL719_RS02450 WP_033155843.1 549301..550167(+) (pilD) [Methylomonas sp. LW13]
ATGGTTTTTGCCACTCTACAACACTATCCGCTGTTTCTGATCAGCGTCGTTTGCGTGCTGGGCCTTCTGGTCGGCAGCTT
CCTAAACGTGGTTATTTACCGTTTGCCGGTCATGATGCAGACGGGTTGGCGGCGCGAATGCCGAGAATATTTGCAGCTCC
CGCAGGATGAAGCAAGCGAAGTGTTCAACCTGATGTTGCCGGCCTCGCATTGCCCGTCGTGCAAAACCGAAATCAAAGCC
TATCAAAACATTCCGGTCATCAGTTACCTGTTGTTGGGCGGCAAATGCGCACATTGTGGGGTGAAAATCGCCATCCGTTA
CCCGCTTATCGAAGCGTTTACCGGTTTATGTTCGGCCGTGGTGGCTTGGCATTTCGGTTACGGTTGGGCTTTAGCGTTTG
CATTACCTCTGACTTGGTGTTTGATCGCATTGAGTTTTATCGACATCGATCAGCAATTATTACCGGATTCGATCACGCTG
CCGATGCTGTGGCTGGGCCTGTTGTTAAGCCTGTTTGATATATACACGAATAGCCACGACGCGATCATCGGTGCGGTGGC
AGGCTATTTGAGCCTGTGGAGCGTTTATCAACTTTTTAAATTGCTGACCGGCAAGGAAGGGATGGGGTATGGCGATTTCA
AACTGCTGGCGCTGTTCGGTGCCTGGCTGGGCTGGCAATATCTGCCGCTGATTATTCTCCTGTCATCTTTGGTGGGCGCT
GTCATCGGCATTGCGATGATCGTGTTCGGCAAACGCGATGCCGGCAAACCGATACCCTTCGGGCCTTATCTGGCCATGGC
CGGCTGGCTGGCGATGTTGTTCGGGCCGGAGCTGAATGCCTTATATCTGCATAGTGCCGGTTTGTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(19-125)

Type IV leader peptidase family (Peptidase_A24)

(137-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

52.297

98.264

0.514
  pilD Acinetobacter baumannii D1279779

55.224

93.056

0.514
  pilD Acinetobacter nosocomialis M2

54.851

93.056

0.51
  pilD Vibrio cholerae strain A1552

51.773

97.917

0.507
  pilD Neisseria gonorrhoeae MS11

54.231

90.278

0.49

Multiple sequence alignment