Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   EB817_RS00770 Genome accession   NZ_CP033336
Coordinates   115275..116213 (+) Length   312 a.a.
NCBI ID   WP_129304381.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain TSPY165     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 110275..121213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EB817_RS00760 (EB817_00760) rpoC 111024..114665 (+) 3642 WP_063631481.1 DNA-directed RNA polymerase subunit beta' -
  EB817_RS00765 (EB817_00765) - 114817..115182 (+) 366 WP_002986560.1 DUF1033 family protein -
  EB817_RS00770 (EB817_00770) comYA 115275..116213 (+) 939 WP_129304381.1 competence type IV pilus ATPase ComGA Machinery gene
  EB817_RS00775 (EB817_00775) comYB 116149..117183 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  EB817_RS00780 (EB817_00780) comYC 117185..117511 (+) 327 WP_011528161.1 competence type IV pilus major pilin ComGC Machinery gene
  EB817_RS00785 (EB817_00785) comGD 117486..117914 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  EB817_RS00790 (EB817_00790) comGE 117871..118155 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  EB817_RS00795 (EB817_00795) comYF 118148..118582 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  EB817_RS00800 (EB817_00800) comGG 118566..118892 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  EB817_RS00805 (EB817_00805) comYH 118990..119943 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  EB817_RS00810 (EB817_00810) - 120002..121198 (+) 1197 WP_002987791.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35032.31 Da        Isoelectric Point: 8.5780

>NTDB_id=323050 EB817_RS00770 WP_129304381.1 115275..116213(+) (comYA) [Streptococcus pyogenes strain TSPY165]
MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERKLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YVRLLELGVTKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=323050 EB817_RS00770 WP_129304381.1 115275..116213(+) (comYA) [Streptococcus pyogenes strain TSPY165]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAAATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTATTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTTAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAAGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAGTTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAGATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGTAAGATTGTTAGAACTTGGTGTAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA140

61.093

99.679

0.609
  comYA Streptococcus mutans UA159

61.093

99.679

0.609
  comYA Streptococcus gordonii str. Challis substr. CH1

60.45

99.679

0.603
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.323

99.359

0.599
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.807

99.679

0.596
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

59.164

99.679

0.59
  comGA/cglA/cilD Streptococcus pneumoniae D39

59.164

99.679

0.59
  comGA/cglA/cilD Streptococcus pneumoniae R6

59.164

99.679

0.59
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

59.164

99.679

0.59
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

38.966

92.949

0.362

Multiple sequence alignment