Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   EB817_RS00795 Genome accession   NZ_CP033336
Coordinates   118148..118582 (+) Length   144 a.a.
NCBI ID   WP_002992738.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain TSPY165     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 113148..123582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EB817_RS00765 (EB817_00765) - 114817..115182 (+) 366 WP_002986560.1 DUF1033 family protein -
  EB817_RS00770 (EB817_00770) comYA 115275..116213 (+) 939 WP_129304381.1 competence type IV pilus ATPase ComGA Machinery gene
  EB817_RS00775 (EB817_00775) comYB 116149..117183 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  EB817_RS00780 (EB817_00780) comYC 117185..117511 (+) 327 WP_011528161.1 competence type IV pilus major pilin ComGC Machinery gene
  EB817_RS00785 (EB817_00785) comGD 117486..117914 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  EB817_RS00790 (EB817_00790) comGE 117871..118155 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  EB817_RS00795 (EB817_00795) comYF 118148..118582 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  EB817_RS00800 (EB817_00800) comGG 118566..118892 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  EB817_RS00805 (EB817_00805) comYH 118990..119943 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  EB817_RS00810 (EB817_00810) - 120002..121198 (+) 1197 WP_002987791.1 acetate kinase -
  EB817_RS00815 (EB817_00815) - 121385..121693 (+) 309 Protein_111 hypothetical protein -
  EB817_RS00820 (EB817_00820) proC 121776..122546 (-) 771 WP_129304383.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16645.27 Da        Isoelectric Point: 10.2526

>NTDB_id=323053 EB817_RS00795 WP_002992738.1 118148..118582(+) (comYF) [Streptococcus pyogenes strain TSPY165]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=323053 EB817_RS00795 WP_002992738.1 118148..118582(+) (comYF) [Streptococcus pyogenes strain TSPY165]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment