Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EAO82_RS17830 Genome accession   NZ_CP033116
Coordinates   3883315..3884577 (+) Length   420 a.a.
NCBI ID   WP_096345137.1    Uniprot ID   A0AA91U5I6
Organism   Halopseudomonas pelagia strain Kongs-67     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3878315..3889577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAO82_RS17815 (EAO82_17920) - 3880187..3880657 (-) 471 WP_185153062.1 pilin -
  EAO82_RS17820 (EAO82_17925) - 3880873..3881328 (-) 456 WP_096345135.1 pilin -
  EAO82_RS17825 (EAO82_17930) pilB 3881608..3883311 (+) 1704 WP_096345136.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EAO82_RS17830 (EAO82_17935) pilC 3883315..3884577 (+) 1263 WP_096345137.1 type II secretion system F family protein Machinery gene
  EAO82_RS17835 (EAO82_17940) pilD 3884581..3885453 (+) 873 WP_096345138.1 prepilin peptidase Machinery gene
  EAO82_RS17840 (EAO82_17945) - 3885514..3885828 (+) 315 WP_096345139.1 transcriptional regulator -
  EAO82_RS17845 (EAO82_17950) - 3885821..3886099 (+) 279 WP_096345140.1 helix-turn-helix domain-containing protein -
  EAO82_RS17850 (EAO82_17955) coaE 3886133..3886759 (+) 627 WP_096345141.1 dephospho-CoA kinase -
  EAO82_RS17855 (EAO82_17960) yacG 3886756..3886944 (+) 189 WP_096345142.1 DNA gyrase inhibitor YacG -
  EAO82_RS17860 (EAO82_17965) - 3886957..3887976 (+) 1020 WP_096345143.1 Nudix family hydrolase -
  EAO82_RS17865 (EAO82_17970) ispH 3887994..3888941 (-) 948 WP_096345144.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45668.70 Da        Isoelectric Point: 9.9572

>NTDB_id=321630 EAO82_RS17830 WP_096345137.1 3883315..3884577(+) (pilC) [Halopseudomonas pelagia strain Kongs-67]
MAQQAAVKRKLASPKTKAKVDKTVPFKWEGKDRKGTKVAGELQGLNVALVKAQLRKQGILATKVTKKSTLFGTRAKKIKP
LDIAFFTRQLSTMMAAGVPIIQSFDIIAEGTENPNLQKLINEIKADVAAGNTLADSLRAHPKYFDDLFCNLVESGEQSGK
LESLLSRIATYKEKTEALKAKIKKAMTYPIAVVVVAIIVTAILLLKVVPQFQMVFSSFGANLPAFTLWVMGISEWLQAWW
FIALVVIIVIGYAYTQAHVRSKKFRDAEDRALLKAPVVGDIVYKAAVARYARTLSTTFAAGVPLVEALDSVAGAAGNVVF
YNAIMQIKEDVSAGSQLNFSMRTTNVFPSMAVQMAGIGEESGNLDGMLEKVADYYESEVDNAVDNLTTLLEPIIMVVLGV
LVGGLIIAMYLPIFQLGNVV

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=321630 EAO82_RS17830 WP_096345137.1 3883315..3884577(+) (pilC) [Halopseudomonas pelagia strain Kongs-67]
ATGGCGCAACAAGCCGCAGTAAAAAGAAAATTGGCGTCGCCCAAAACAAAGGCAAAAGTTGATAAAACTGTACCTTTTAA
GTGGGAAGGCAAGGATCGCAAAGGCACAAAAGTAGCTGGCGAATTGCAGGGGCTTAATGTTGCTCTGGTAAAAGCTCAAT
TGCGCAAACAAGGCATTCTGGCGACCAAGGTCACAAAAAAGTCGACGTTGTTTGGCACCCGAGCGAAAAAGATCAAGCCA
CTTGATATCGCATTTTTTACACGTCAGCTCTCTACTATGATGGCGGCGGGCGTCCCAATCATTCAGTCGTTCGACATTAT
TGCTGAGGGAACTGAAAACCCCAATTTGCAGAAATTGATCAACGAGATTAAAGCAGATGTGGCGGCAGGTAACACCTTGG
CGGATTCGCTTAGAGCTCACCCTAAATATTTTGACGATTTGTTTTGTAACCTCGTCGAGTCAGGCGAGCAGTCAGGCAAG
CTGGAATCATTGCTTTCACGCATCGCCACGTACAAAGAAAAAACCGAAGCCCTGAAAGCGAAGATCAAGAAGGCCATGAC
CTATCCGATCGCGGTAGTCGTGGTCGCCATCATCGTTACCGCCATTCTGCTACTCAAGGTAGTACCTCAGTTCCAGATGG
TTTTTTCCAGCTTTGGAGCAAATCTTCCCGCCTTTACTCTTTGGGTAATGGGAATATCTGAATGGCTTCAGGCTTGGTGG
TTTATAGCGCTGGTCGTTATCATCGTCATCGGTTATGCCTACACTCAAGCCCATGTGCGGTCCAAGAAATTTCGTGATGC
GGAAGACCGCGCTTTGCTGAAGGCCCCGGTTGTTGGCGATATTGTGTATAAAGCGGCTGTGGCCCGATATGCCCGTACCC
TGTCGACGACCTTCGCCGCAGGCGTACCATTGGTTGAAGCACTGGATTCCGTCGCAGGCGCAGCAGGCAATGTCGTTTTC
TACAACGCCATTATGCAAATCAAAGAAGACGTTTCTGCTGGCTCTCAGCTGAATTTCTCGATGCGTACCACAAATGTCTT
TCCATCAATGGCCGTGCAAATGGCGGGCATTGGGGAAGAGTCCGGCAACTTGGATGGCATGCTGGAAAAGGTAGCCGACT
ACTACGAAAGCGAAGTCGATAATGCCGTGGACAACCTCACCACGCTTCTCGAGCCAATTATCATGGTAGTACTTGGCGTG
CTGGTTGGCGGCCTTATCATTGCCATGTACTTGCCAATCTTCCAGCTTGGTAATGTTGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(86-209)

Type II secretion system (T2SS), protein F (T2SSF)

(291-412)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

70.149

95.714

0.671
  pilC Acinetobacter baylyi ADP1

59.606

96.667

0.576
  pilC Acinetobacter baumannii D1279779

59.446

94.524

0.562
  pilC Legionella pneumophila strain ERS1305867

53.695

96.667

0.519
  pilG Neisseria gonorrhoeae MS11

44.444

94.286

0.419
  pilG Neisseria meningitidis 44/76-A

44.444

94.286

0.419
  pilC Vibrio cholerae strain A1552

40.931

97.143

0.398
  pilC Vibrio campbellii strain DS40M4

41.353

95

0.393

Multiple sequence alignment