Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EAO82_RS17825 Genome accession   NZ_CP033116
Coordinates   3881608..3883311 (+) Length   567 a.a.
NCBI ID   WP_096345136.1    Uniprot ID   A0AA91U4Z7
Organism   Halopseudomonas pelagia strain Kongs-67     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3876608..3888311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAO82_RS17810 (EAO82_17915) - 3877072..3880047 (-) 2976 WP_096345134.1 transglycosylase domain-containing protein -
  EAO82_RS17815 (EAO82_17920) - 3880187..3880657 (-) 471 WP_185153062.1 pilin -
  EAO82_RS17820 (EAO82_17925) - 3880873..3881328 (-) 456 WP_096345135.1 pilin -
  EAO82_RS17825 (EAO82_17930) pilB 3881608..3883311 (+) 1704 WP_096345136.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EAO82_RS17830 (EAO82_17935) pilC 3883315..3884577 (+) 1263 WP_096345137.1 type II secretion system F family protein Machinery gene
  EAO82_RS17835 (EAO82_17940) pilD 3884581..3885453 (+) 873 WP_096345138.1 prepilin peptidase Machinery gene
  EAO82_RS17840 (EAO82_17945) - 3885514..3885828 (+) 315 WP_096345139.1 transcriptional regulator -
  EAO82_RS17845 (EAO82_17950) - 3885821..3886099 (+) 279 WP_096345140.1 helix-turn-helix domain-containing protein -
  EAO82_RS17850 (EAO82_17955) coaE 3886133..3886759 (+) 627 WP_096345141.1 dephospho-CoA kinase -
  EAO82_RS17855 (EAO82_17960) yacG 3886756..3886944 (+) 189 WP_096345142.1 DNA gyrase inhibitor YacG -
  EAO82_RS17860 (EAO82_17965) - 3886957..3887976 (+) 1020 WP_096345143.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62755.11 Da        Isoelectric Point: 5.2543

>NTDB_id=321629 EAO82_RS17825 WP_096345136.1 3881608..3883311(+) (pilB) [Halopseudomonas pelagia strain Kongs-67]
MDTPALSGLARRIVQDQLLDAQVAAKASKQASQDKIPFITYLVQNKLASARDLAMLCAEEFGYPYCDLAAIDKESQPQDL
VSEKLIRQHSVLPLFKRGSRLFLGISDPTNQQALSDVQFSTGLMTDAVIVEDDKLHTLIEKFLESPTGGLGDMSDGELDD
LDIQNVKDDEKKDGGTSDADDAPIVRFVNKMLLDAVRMGSSDLHFEPYEKVYRVRFRTDGILHEMAKPPIQLGVRIAARL
KVMSSMDMAERRKPQDGRIKMKISKNKAIDFRVNTLPTLWGEKVVLRILDAESAKMGIDALGYEDDQKEMYMSALARPQG
MILVTGPTGSGKTVSLYTGLNILNTVERNISTAEDPVEINLEGVNQVNVNPKQGLDFAKALRAFLRQDPDIIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARRLCKSCKKPMEIPNEVLLEEGFTQ
EKIDAGITIYSPVGCENCKDGYKGRVGIYEVVKITQAMQRIIMEDGNSIEITEVAQKEGFRNLRQSALMKAEQGVTSLAE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=321629 EAO82_RS17825 WP_096345136.1 3881608..3883311(+) (pilB) [Halopseudomonas pelagia strain Kongs-67]
ATGGATACCCCCGCGCTCTCCGGTCTGGCCCGGCGTATTGTTCAGGACCAGTTGCTCGACGCTCAGGTTGCCGCTAAGGC
CAGCAAGCAAGCTAGTCAGGACAAGATTCCCTTTATCACCTACCTCGTCCAGAACAAGCTGGCCAGCGCTCGCGATCTGG
CAATGCTCTGTGCGGAAGAGTTTGGCTATCCCTATTGCGACCTTGCGGCGATTGACAAGGAATCTCAACCACAGGACTTA
GTCAGCGAAAAGCTGATTCGCCAACATTCTGTGTTGCCGCTGTTCAAGCGCGGTTCACGCTTGTTTCTCGGCATATCAGA
CCCTACTAATCAGCAAGCCCTGAGCGACGTTCAGTTCAGTACGGGATTGATGACGGACGCCGTTATCGTAGAAGACGATA
AGCTGCATACGCTGATCGAGAAGTTCCTCGAAAGCCCCACAGGTGGGCTTGGGGATATGAGCGACGGCGAGCTCGACGAC
CTGGATATCCAGAACGTCAAAGACGATGAAAAAAAGGACGGCGGCACGAGTGATGCCGATGATGCGCCCATCGTTCGCTT
CGTGAATAAAATGCTGTTGGACGCAGTTCGTATGGGCTCATCTGACCTTCATTTTGAGCCGTATGAAAAGGTCTATCGGG
TACGCTTTCGTACCGACGGCATATTGCACGAAATGGCCAAGCCACCGATCCAATTAGGCGTTCGTATTGCGGCTCGCCTC
AAGGTGATGTCATCCATGGATATGGCTGAGCGGCGTAAACCGCAGGATGGCCGTATCAAAATGAAAATATCCAAGAACAA
AGCGATCGATTTTCGCGTCAATACGCTGCCTACGCTATGGGGCGAGAAAGTAGTGCTGCGTATTCTCGACGCAGAAAGCG
CCAAGATGGGCATCGACGCGCTTGGCTATGAAGATGATCAAAAAGAGATGTATATGTCTGCTCTTGCTCGCCCGCAAGGC
ATGATCTTGGTGACTGGGCCAACTGGCTCAGGGAAAACCGTTTCTCTTTACACCGGCTTGAATATTCTCAACACTGTTGA
GCGCAATATCTCTACTGCAGAAGACCCAGTCGAGATCAACCTTGAAGGTGTAAACCAGGTCAACGTTAACCCAAAACAGG
GGCTGGATTTTGCCAAGGCTCTTAGGGCGTTTTTGCGTCAGGATCCGGATATCATCATGGTGGGCGAAATTCGCGACTTG
GAAACGGCCGAAATCGCCATTAAGGCTGCACAAACAGGCCACATGGTGATGTCGACCCTTCACACCAATAGTGCTGCCGA
GACGCTTACGCGCTTGAGGAATATGGGCGTTCCTTCCTTCAATATCGCCACATCGGTAAACCTGATTATTGCCCAGCGGC
TGGCACGGCGGCTATGCAAGTCCTGCAAAAAGCCAATGGAGATACCTAATGAAGTACTGCTTGAGGAAGGATTCACGCAG
GAAAAAATAGACGCTGGGATCACAATTTATAGTCCTGTAGGTTGCGAAAATTGCAAAGACGGTTATAAAGGACGTGTTGG
CATCTATGAGGTAGTCAAAATCACCCAGGCGATGCAGCGAATCATTATGGAGGACGGCAACTCCATTGAGATTACTGAAG
TAGCACAGAAAGAAGGATTCAGAAACTTGCGCCAATCCGCCCTGATGAAAGCGGAACAGGGTGTTACCAGCCTGGCGGAA
GTCAACCGAGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.035

100

0.607
  pilB Acinetobacter baylyi ADP1

59.436

100

0.594
  pilB Legionella pneumophila strain ERS1305867

56.36

99.824

0.563
  pilB Vibrio cholerae strain A1552

48.955

100

0.496
  pilF Neisseria gonorrhoeae MS11

49.381

99.647

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

51.803

92.945

0.481
  pilB Vibrio campbellii strain DS40M4

50.662

93.298

0.473
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.848

92.945

0.37

Multiple sequence alignment