Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   TFGB2_RS12515 Genome accession   NZ_CP032429
Coordinates   2109296..2109805 (-) Length   169 a.a.
NCBI ID   WP_134797946.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain TFG-B2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2104296..2114805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TFGB2_RS12495 (TFGB2_02200) msrAB 2104786..2106354 (-) 1569 WP_003687002.1 bifunctional peptide-methionine (S)-S-oxide reductase MsrA/peptide-methionine (R)-S-oxide reductase MsrB -
  TFGB2_RS12500 (TFGB2_02201) pilA 2106500..2107753 (+) 1254 WP_003696286.1 signal recognition particle-docking protein FtsY Machinery gene
  TFGB2_RS13520 - 2107830..2108003 (-) 174 WP_169300794.1 hypothetical protein -
  TFGB2_RS12505 (TFGB2_02202) - 2108017..2108696 (-) 680 Protein_2141 opacity family porin -
  TFGB2_RS12515 (TFGB2_02203) pilE 2109296..2109805 (-) 510 WP_134797946.1 pilin Machinery gene
  TFGB2_RS12520 - 2110174..2110317 (-) 144 WP_003691943.1 hypothetical protein -
  TFGB2_RS14195 - 2110620..2110723 (-) 104 Protein_2144 pilin -
  TFGB2_RS12535 (TFGB2_02204) pilE 2110861..2111292 (-) 432 WP_050170900.1 pilin Machinery gene
  TFGB2_RS12540 (TFGB2_02205) lpxC 2111399..2112322 (-) 924 WP_003687007.1 UDP-3-O-acyl-N-acetylglucosamine deacetylase -
  TFGB2_RS12545 (TFGB2_02206) - 2112683..2112913 (-) 231 Protein_2147 hypothetical protein -
  TFGB2_RS12550 (TFGB2_02207) - 2112910..2113653 (-) 744 WP_003687014.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  TFGB2_RS12555 (TFGB2_02208) gmhB 2113683..2114246 (-) 564 WP_003687015.1 D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase -

Sequence

Protein


Download         Length: 169 a.a.        Molecular weight: 17901.18 Da        Isoelectric Point: 5.8919

>NTDB_id=315795 TFGB2_RS12515 WP_134797946.1 2109296..2109805(-) (pilE) [Neisseria gonorrhoeae strain TFG-B2]
MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAILLAEGQKSAVTEYYLNNGKWPANNGDAGVASSSSII
GKYVESVTVTNGVVTAKMKSDGVNKEIQGKKLSLWAKREDGSVKWFCGQPVTRTGNADDDVTDDATAGNNKIDTKHLPST
CRDTSSAGT

Nucleotide


Download         Length: 510 bp        

>NTDB_id=315795 TFGB2_RS12515 WP_134797946.1 2109296..2109805(-) (pilE) [Neisseria gonorrhoeae strain TFG-B2]
ATGAATACCCTTCAAAAAGGCTTTACCCTTATCGAGCTGATGATTGTGATCGCCATCGTCGGCATTTTGGCGGCAGTCGC
CCTTCCCGCCTACCAAGACTACACCGCCCGCGCGCAAGTTTCCGAAGCCATCCTTTTGGCCGAAGGTCAAAAATCAGCCG
TTACCGAGTATTACCTGAATAACGGCAAATGGCCCGCAAACAACGGTGATGCCGGCGTGGCATCTTCTTCATCAATCATA
GGCAAATATGTTGAAAGCGTTACGGTCACAAACGGCGTCGTTACCGCCAAAATGAAATCAGACGGCGTAAACAAAGAAAT
CCAAGGCAAAAAACTCTCCCTGTGGGCCAAGCGTGAAGACGGTTCGGTAAAATGGTTCTGCGGACAGCCGGTTACGCGCA
CCGGCAACGCCGACGACGACGTTACCGACGACGCCACCGCCGGCAACAACAAAATCGACACCAAGCACCTGCCGTCAACC
TGCCGTGATACGTCATCTGCCGGTACGTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-162)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

82.036

98.817

0.811
  pilE Neisseria gonorrhoeae MS11

79.641

98.817

0.787
  pilA/pilA1 Eikenella corrodens VA1

36.782

100

0.379
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

33.684

100

0.379
  pilA2 Legionella pneumophila strain ERS1305867

38.994

94.083

0.367
  pilA2 Legionella pneumophila str. Paris

38.994

94.083

0.367
  comP Acinetobacter baylyi ADP1

36.747

98.225

0.361

Multiple sequence alignment