Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MADE_RS14780 Genome accession   NC_011138
Coordinates   3333986..3335194 (+) Length   402 a.a.
NCBI ID   WP_012519429.1    Uniprot ID   F2GCZ0
Organism   Alteromonas mediterranea DE     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3328986..3340194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MADE_RS14755 (MADE_1014990) ampE 3329933..3330802 (-) 870 WP_012519424.1 beta-lactamase regulator AmpE -
  MADE_RS14760 (MADE_1014995) ampD 3330833..3331369 (-) 537 WP_012519425.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MADE_RS14765 (MADE_1015000) - 3331545..3332063 (+) 519 WP_012519426.1 retropepsin-like aspartic protease family protein -
  MADE_RS14770 (MADE_1015005) nadC 3332083..3332937 (+) 855 WP_012519427.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MADE_RS14775 (MADE_1015010) - 3333473..3333898 (+) 426 WP_012519428.1 pilin -
  MADE_RS14780 (MADE_1015015) pilC 3333986..3335194 (+) 1209 WP_012519429.1 type II secretion system F family protein Machinery gene
  MADE_RS14785 (MADE_1015020) pilD 3335312..3336205 (+) 894 WP_012519430.1 prepilin peptidase Machinery gene
  MADE_RS14790 (MADE_000001022595) coaE 3336227..3336841 (+) 615 WP_041912943.1 dephospho-CoA kinase -
  MADE_RS14795 (MADE_1015035) zapD 3337055..3337807 (+) 753 WP_012519433.1 cell division protein ZapD -
  MADE_RS14800 (MADE_1015040) yacG 3337898..3338125 (+) 228 WP_012519434.1 DNA gyrase inhibitor YacG -
  MADE_RS14805 (MADE_1015045) - 3338319..3339191 (+) 873 WP_012519435.1 aspartoacylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43710.31 Da        Isoelectric Point: 9.7149

>NTDB_id=31417 MADE_RS14780 WP_012519429.1 3333986..3335194(+) (pilC) [Alteromonas mediterranea DE]
MAKATATFTWQGKDRNGQSRKGEISATSLSEAKNLLRRQGISANKVKKLAQPLFGRTQKIKPADISVISRQIATMLAAGV
TLIQSLEMIAQGHANPAVRKLLGEITDEVKSGNPLSSALRKHPLYFDDLYCDLVYTGEQSGALETIYDRIATYKEKAEAL
KSKIKKAMFYPIAVLVVAFIVTTILLVFVVPQFEEIFSSFGAELPAFTQFVLAISRFVQDYGIFIAMGVAAAGFMFVRAH
RRSQKLRDTVDRNILKIPVIGEILKKASIARFTRTLATTFAAGVPLIGALESAAGASGNAVYRDAILYIRKEVAGGMPMH
VAMRATQVFPDMVTQMIAIGEESGSVDEMLSKIATIYEAEVDDMVDGLTSLLEPMIMAVLGVVIGGLIVAMYLPIFQMGN
VV

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=31417 MADE_RS14780 WP_012519429.1 3333986..3335194(+) (pilC) [Alteromonas mediterranea DE]
ATGGCAAAAGCAACGGCAACGTTTACTTGGCAAGGAAAAGACAGGAATGGTCAATCTCGCAAAGGTGAAATTTCGGCCAC
CTCGCTTTCTGAAGCCAAAAACCTTTTGCGTCGCCAAGGGATTTCTGCGAATAAGGTAAAAAAACTAGCTCAACCACTTT
TTGGCAGAACTCAAAAAATAAAACCTGCCGATATATCTGTCATATCACGACAAATTGCCACCATGCTAGCCGCAGGTGTT
ACGCTGATTCAATCTCTAGAAATGATAGCACAAGGCCATGCAAACCCTGCAGTGCGAAAACTGCTTGGCGAAATTACGGA
TGAAGTCAAATCCGGTAATCCACTTTCTTCTGCGCTTCGAAAACACCCGCTTTATTTTGACGATTTATACTGTGATTTGG
TTTACACGGGCGAACAGTCAGGTGCCCTTGAAACCATTTACGATCGTATTGCCACCTATAAAGAAAAAGCCGAAGCACTA
AAATCAAAGATCAAAAAAGCGATGTTTTACCCTATCGCGGTTTTGGTCGTTGCTTTTATTGTTACGACTATTCTTCTTGT
TTTCGTTGTGCCTCAGTTTGAAGAAATCTTTAGCAGCTTTGGCGCTGAACTCCCTGCGTTTACCCAGTTCGTACTGGCTA
TATCGCGGTTTGTACAGGATTACGGTATTTTTATCGCCATGGGCGTGGCAGCCGCAGGCTTTATGTTTGTGCGCGCGCAC
AGGCGCAGTCAAAAGCTTCGCGATACGGTAGACCGGAATATTTTAAAAATTCCTGTTATCGGCGAGATACTTAAAAAAGC
CAGTATCGCACGCTTTACCCGAACCCTTGCCACCACCTTTGCGGCAGGTGTACCGCTAATAGGCGCGTTAGAGTCAGCTG
CAGGGGCCTCGGGAAATGCAGTATACCGCGACGCTATTTTATATATTCGCAAAGAAGTGGCCGGTGGTATGCCTATGCAT
GTAGCCATGCGCGCTACCCAAGTATTTCCAGACATGGTGACGCAAATGATAGCCATAGGTGAAGAGTCTGGCTCTGTCGA
TGAGATGCTAAGTAAAATTGCCACCATTTACGAAGCAGAAGTTGATGACATGGTAGACGGCTTAACCAGCTTGCTTGAGC
CTATGATCATGGCCGTACTTGGCGTGGTTATTGGCGGCTTGATTGTGGCCATGTACCTGCCTATATTCCAGATGGGTAAC
GTGGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F2GCZ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.543

99.005

0.58
  pilC Acinetobacter baumannii D1279779

52.593

100

0.53
  pilC Legionella pneumophila strain ERS1305867

52.632

99.254

0.522
  pilC Acinetobacter baylyi ADP1

52.645

98.756

0.52
  pilC Vibrio cholerae strain A1552

46.059

100

0.465
  pilG Neisseria gonorrhoeae MS11

44.581

100

0.45
  pilG Neisseria meningitidis 44/76-A

43.812

100

0.44
  pilC Vibrio campbellii strain DS40M4

43.073

98.756

0.425
  pilC Thermus thermophilus HB27

36.25

99.502

0.361

Multiple sequence alignment