Detailed information NTG0031418

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MADE_RS14785
• Genome accession: NC_011138
• Coordinates: 3335312..3336205 (+)
• Length: 297 a.a.
• NCBI ID: WP_012519430.1
• Uniprot ID: F2GCZ1
• Organism: Alteromonas mediterranea DE
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3330312..3341205

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MADE_RS14760 (MADE_1014995)	ampD	3330833..3331369 (-)	537	WP_012519425.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
MADE_RS14765 (MADE_1015000)	-	3331545..3332063 (+)	519	WP_012519426.1	retropepsin-like aspartic protease family protein	-
MADE_RS14770 (MADE_1015005)	nadC	3332083..3332937 (+)	855	WP_012519427.1	carboxylating nicotinate-nucleotide diphosphorylase	-
MADE_RS14775 (MADE_1015010)	-	3333473..3333898 (+)	426	WP_012519428.1	pilin	-
MADE_RS14780 (MADE_1015015)	pilC	3333986..3335194 (+)	1209	WP_012519429.1	type II secretion system F family protein	Machinery gene
MADE_RS14785 (MADE_1015020)	pilD	3335312..3336205 (+)	894	WP_012519430.1	prepilin peptidase	Machinery gene
MADE_RS14790 (MADE_000001022595)	coaE	3336227..3336841 (+)	615	WP_041912943.1	dephospho-CoA kinase	-
MADE_RS14795 (MADE_1015035)	zapD	3337055..3337807 (+)	753	WP_012519433.1	cell division protein ZapD	-
MADE_RS14800 (MADE_1015040)	yacG	3337898..3338125 (+)	228	WP_012519434.1	DNA gyrase inhibitor YacG	-
MADE_RS14805 (MADE_1015045)	-	3338319..3339191 (+)	873	WP_012519435.1	aspartoacylase	-
MADE_RS14810 (MADE_1015050)	-	3339422..3340711 (-)	1290	WP_012519436.1	methyl-accepting chemotaxis protein	-
MADE_RS14815 (MADE_1015055)	-	3340721..3341089 (-)	369	WP_012519437.1	response regulator	-

Sequence

Protein

• Length: 297 a.a.
• Molecular weight: 32753.77 Da
• Isoelectric Point: 5.2127

>NTDB_id=31418 MADE_RS14785 WP_012519430.1 3335312..3336205(+) (pilD) [Alteromonas mediterranea DE]
MDAIITLSQLYPAIFYTLIFLVSLMVGSFLNVVIYRLPIMMERSWQQEYQSYFTDGDTSLDTPNAPSQDDTFNLVKPDST
CPSCGHKIRAWENIPVISYLFLKGKCAKCKVAISVRYPLVELFTAIACTFAAYQFGATPQALWAVVFTYVLVALLFIDLD
KMLLPDQLTLPLLWLGLLLSTQNIFVGTTDAIIGATAGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAALGAFTGWQGLPV
IILLSSLVGAIAGIAIMVVQNKGKSLAIPFGPYLAVAGWLTLLFKAEIITTYLDYVL

Nucleotide

• Length: 894 bp

>NTDB_id=31418 MADE_RS14785 WP_012519430.1 3335312..3336205(+) (pilD) [Alteromonas mediterranea DE]
ATGGACGCTATAATAACCCTTAGCCAGCTATACCCCGCTATATTCTATACCCTTATCTTCTTAGTGAGCTTAATGGTTGG
CAGCTTTTTAAATGTAGTGATCTACCGCTTACCCATTATGATGGAGCGCAGCTGGCAGCAGGAATACCAAAGCTATTTTA
CCGACGGTGATACCAGTTTAGATACACCTAACGCTCCATCGCAAGACGATACCTTTAACCTGGTAAAACCAGACAGCACC
TGCCCCAGCTGCGGCCACAAAATTCGCGCATGGGAAAACATTCCTGTTATTAGTTACCTATTTCTAAAGGGCAAATGTGC
TAAGTGCAAGGTAGCTATTTCAGTACGCTATCCGCTGGTTGAGCTTTTCACTGCAATCGCCTGCACCTTCGCAGCCTATC
AATTTGGTGCAACGCCTCAAGCGCTTTGGGCCGTGGTTTTTACCTACGTACTCGTGGCTTTGCTATTTATCGATTTAGAT
AAAATGTTGTTGCCAGACCAGCTCACGCTCCCTTTGCTTTGGCTTGGGCTGCTGTTAAGTACTCAAAACATTTTTGTTGG
TACGACCGACGCCATTATTGGCGCGACGGCAGGCTATTTAAGCTTGTGGTCAGTATATTGGTTATTTAAGCTTGTTACGG
GCAAGGAAGGCATGGGCTATGGCGATTTTAAATTGCTTGCCGCCTTAGGTGCGTTCACAGGCTGGCAAGGGCTCCCCGTT
ATCATCTTACTTTCATCACTGGTGGGCGCCATTGCAGGTATTGCTATTATGGTTGTGCAAAATAAGGGGAAATCCTTAGC
CATCCCCTTTGGCCCTTACCTTGCTGTAGCAGGGTGGCTTACGCTGCTTTTTAAAGCTGAAATTATCACTACTTACCTTG
ATTACGTGCTTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-135)

• Type IV leader peptidase family (Peptidase_A24) (146-254)

3D structure

• AlphaFold DB: F2GCZ1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.633	97.306	0.522
pilD	Vibrio campbellii strain DS40M4	53.448	97.643	0.522
pilD	Acinetobacter baumannii D1279779	51.786	94.276	0.488
pilD	Acinetobacter nosocomialis M2	51.429	94.276	0.485
pilD	Neisseria gonorrhoeae MS11	49.615	87.542	0.434