Detailed information NTG0314100

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LL1196_RS12110
• Genome accession: NZ_CP032148
• Coordinates: 2313034..2313459 (-)
• Length: 141 a.a.
• NCBI ID: WP_021216262.1
• Uniprot ID: A0A896TCW7
• Organism: Lactococcus cremoris strain 1196
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2308034..2318459

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL1196_RS12085 (LL1196_2422)	-	2309637..2310446 (-)	810	WP_011677177.1	metal ABC transporter permease	-
LL1196_RS12090 (LL1196_2423)	-	2310439..2311176 (-)	738	WP_011677178.1	metal ABC transporter ATP-binding protein	-
LL1196_RS12095 (LL1196_2424)	-	2311355..2312197 (-)	843	WP_021216263.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
LL1196_RS12100 (LL1196_2425)	-	2312194..2312631 (-)	438	WP_011677180.1	zinc-dependent MarR family transcriptional regulator	-
LL1196_RS12105 (LL1196_03775)	comGG	2312711..2313010 (-)	300	WP_011677181.1	competence type IV pilus minor pilin ComGG	Machinery gene
LL1196_RS12110 (LL1196_2426)	comGF	2313034..2313459 (-)	426	WP_021216262.1	competence type IV pilus minor pilin ComGF	Machinery gene
LL1196_RS12115 (LL1196_2427)	comGE	2313443..2313739 (-)	297	WP_021164977.1	competence type IV pilus minor pilin ComGE	Machinery gene
LL1196_RS12120 (LL1196_03780)	comGD	2313711..2313899 (-)	189	WP_014573336.1	hypothetical protein	Machinery gene
LL1196_RS12125 (LL1196_2428)	comGC	2314101..2314451 (-)	351	WP_050574401.1	competence type IV pilus major pilin ComGC	Machinery gene
LL1196_RS12130 (LL1196_2429)	comGB	2314496..2315521 (-)	1026	WP_050574400.1	competence type IV pilus assembly protein ComGB	Machinery gene
LL1196_RS12135 (LL1196_2430)	-	2315421..2316242 (-)	822	Protein_2361	ATPase, T2SS/T4P/T4SS family	-
LL1196_RS12140 (LL1196_2431)	-	2316345..2317235 (+)	891	WP_205536422.1	IS982 family transposase	-
LL1196_RS12145 (LL1196_2432)	comGA	2317217..2317408 (-)	192	WP_014573341.1	hypothetical protein	Machinery gene

Sequence

Protein

• Length: 141 a.a.
• Molecular weight: 16282.84 Da
• Isoelectric Point: 7.7759

>NTDB_id=314100 LL1196_RS12110 WP_021216262.1 2313034..2313459(-) (comGF) [Lactococcus cremoris strain 1196]
MKLKIRAFTLLECLVALLAISGSVLVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGAKLDNVNQNFLYVTKDKK
LRFELVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEENLIKITIDFDNGGERVFIYRFTDTK

Nucleotide

• Length: 426 bp

>NTDB_id=314100 LL1196_RS12110 WP_021216262.1 2313034..2313459(-) (comGF) [Lactococcus cremoris strain 1196]
TTGAAGCTCAAAATTAGGGCATTTACTTTACTTGAATGTCTCGTAGCACTTTTGGCAATTTCTGGTTCAGTGCTAGTCAT
CTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGCCAGAATGATAGTAGGAAAGATTGGCAAATATTTTGTG
AACAAATGCGTTCAGAACTCTCCGGAGCAAAATTAGACAATGTGAATCAAAATTTTTTGTATGTGACCAAAGATAAGAAG
TTACGGTTCGAATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATACCAACCGATGCTTTATGATTT
AAAAGGAGCAAAAATTCAGGCAGAAGAAAATTTGATAAAAATAACAATTGATTTTGATAATGGAGGTGAGCGAGTATTTA
TTTATCGATTTACTGATACAAAGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (38-130)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	99.291	100	0.993
comYF	Streptococcus mutans UA140	43.262	100	0.433
comYF	Streptococcus mutans UA159	42.553	100	0.426
comGF/cglF	Streptococcus mitis NCTC 12261	38.462	100	0.39
comGF/cglF	Streptococcus mitis SK321	38.571	99.291	0.383
comGF/cglF	Streptococcus pneumoniae Rx1	37.143	99.291	0.369
comGF/cglF	Streptococcus pneumoniae D39	37.143	99.291	0.369
comGF/cglF	Streptococcus pneumoniae R6	37.143	99.291	0.369
comGF/cglF	Streptococcus pneumoniae TIGR4	37.143	99.291	0.369