Detailed information NTG0314104

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: LL1196_RS12130
• Genome accession: NZ_CP032148
• Coordinates: 2314496..2315521 (-)
• Length: 341 a.a.
• NCBI ID: WP_050574400.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain 1196
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	2314551..2318953	2314496..2315521	flank	-970

Gene organization within MGE regions

Location: 2314496..2318953

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL1196_RS12130 (LL1196_2429)	comGB	2314496..2315521 (-)	1026	WP_050574400.1	competence type IV pilus assembly protein ComGB	Machinery gene
LL1196_RS12135 (LL1196_2430)	-	2315421..2316242 (-)	822	Protein_2361	ATPase, T2SS/T4P/T4SS family	-
LL1196_RS12140 (LL1196_2431)	-	2316345..2317235 (+)	891	WP_205536422.1	IS982 family transposase	-
LL1196_RS12145 (LL1196_2432)	comGA	2317217..2317408 (-)	192	WP_014573341.1	hypothetical protein	Machinery gene

Sequence

Protein

• Length: 341 a.a.
• Molecular weight: 38411.13 Da
• Isoelectric Point: 10.0626

>NTDB_id=314104 LL1196_RS12130 WP_050574400.1 2314496..2315521(-) (comGB) [Lactococcus cremoris strain 1196]
MQTDILRLLRPKGKKLALIKQAKLIQLMGNLLNNGFHLGEVINFLALSKLVEKEFVFKMHQGLSSGNSLSEILDSLSFSK
NVVTQLALVEVHGNLVGTMQLVELHLKKQLKVKKKLIEVATYPVVLLVFLIGIMWGLKNYLLPQIDKGNNFATLLINHLP
LVFFSVGALIVLLTSLSIIIFKHLSALTNFTFLVKVPLVSSFIRLYLTAYFSREWGNLIAQGVELRQIIELMKKQKSRIF
AEVGIKLDLGLKAGQNFEQAVSHFSIFLPELSLMIEYGAIKDKLGLELSVYADECWEQFFSKIDRLMQLIQPLIFIFVAL
MIVLLYAAMSLPIYSNMGSGI

Nucleotide

• Length: 1026 bp

>NTDB_id=314104 LL1196_RS12130 WP_050574400.1 2314496..2315521(-) (comGB) [Lactococcus cremoris strain 1196]
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAATGGTTTCCATTTGGGAGAAGTTATCAATTTTTTAGCTTTATCAAAATTAGTAGAAAAAG
AGTTTGTATTCAAAATGCACCAAGGTCTCAGTTCTGGAAATTCATTATCTGAAATTTTAGATAGTCTCTCTTTTTCTAAA
AATGTTGTGACACAACTTGCTTTGGTAGAAGTGCATGGGAATTTAGTAGGAACCATGCAATTGGTAGAACTGCATTTAAA
AAAGCAGCTTAAGGTCAAAAAGAAATTAATTGAAGTGGCAACTTATCCAGTTGTATTGTTGGTATTTCTCATTGGAATTA
TGTGGGGCTTGAAAAATTATTTATTACCTCAAATTGATAAGGGAAATAATTTTGCTACTTTATTAATTAATCATTTACCG
CTAGTATTTTTTTCTGTTGGTGCTTTAATCGTTTTACTGACAAGTTTATCAATAATAATATTCAAACATCTTTCAGCACT
CACTAATTTTACTTTTTTGGTTAAAGTTCCATTAGTAAGTTCTTTTATCCGCTTGTATTTGACCGCTTATTTCTCCAGAG
AATGGGGAAATTTAATTGCTCAAGGAGTTGAATTGCGACAAATAATAGAATTGATGAAAAAGCAGAAAAGTCGCATTTTT
GCGGAAGTTGGAATTAAACTTGATTTGGGTTTGAAGGCTGGTCAGAATTTTGAGCAAGCTGTCAGTCATTTTTCGATTTT
TTTACCAGAACTTTCTTTGATGATTGAGTACGGCGCGATTAAAGATAAATTAGGGTTGGAGCTTTCTGTTTATGCTGATG
AATGTTGGGAACAATTCTTTTCTAAGATAGACCGTCTAATGCAATTAATACAACCCCTTATTTTTATTTTTGTTGCTTTG
ATGATTGTCTTATTGTATGCAGCGATGTCGTTACCCATTTATTCTAATATGGGTTCTGGAATTTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (25-143)

• Type II secretion system (T2SS), protein F (T2SSF) (211-332)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Lactococcus lactis subsp. cremoris KW2	98.24	100	0.982
comYB	Streptococcus gordonii str. Challis substr. CH1	51.632	98.827	0.51
comGB/cglB	Streptococcus mitis SK321	50.599	97.947	0.496
comGB/cglB	Streptococcus pneumoniae TIGR4	50.299	97.947	0.493
comGB/cglB	Streptococcus pneumoniae Rx1	50.299	97.947	0.493
comGB/cglB	Streptococcus pneumoniae D39	50.299	97.947	0.493
comGB/cglB	Streptococcus pneumoniae R6	50.299	97.947	0.493
comGB/cglB	Streptococcus mitis NCTC 12261	49.701	97.947	0.487
comYB	Streptococcus mutans UA140	51.592	92.082	0.475
comYB	Streptococcus mutans UA159	50.955	92.082	0.469