Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   D2M30_RS13000 Genome accession   NZ_CP032146
Coordinates   2576166..2576780 (-) Length   204 a.a.
NCBI ID   WP_125122985.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain YP6     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2571166..2581780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D2M30_RS12970 (D2M30_12995) rpsT 2571466..2571732 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  D2M30_RS12975 (D2M30_13000) holA 2571750..2572791 (-) 1042 Protein_2526 DNA polymerase III subunit delta -
  D2M30_RS12980 - 2572831..2572985 (-) 155 Protein_2527 hypothetical protein -
  D2M30_RS12985 (D2M30_13005) - 2573026..2573160 (+) 135 WP_003152870.1 YqzM family protein -
  D2M30_RS12990 (D2M30_13010) comEC 2573179..2575530 (-) 2352 WP_125122984.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  D2M30_RS12995 (D2M30_13015) - 2575531..2576100 (-) 570 WP_045510461.1 ComE operon protein 2 -
  D2M30_RS13000 (D2M30_13020) comEA 2576166..2576780 (-) 615 WP_125122985.1 helix-hairpin-helix domain-containing protein Machinery gene
  D2M30_RS13005 (D2M30_13025) comER 2576840..2577661 (+) 822 WP_125122986.1 late competence protein ComER -
  D2M30_RS13010 (D2M30_13030) - 2577730..2578467 (-) 738 WP_125122987.1 class I SAM-dependent methyltransferase -
  D2M30_RS13015 (D2M30_13035) rsfS 2578464..2578820 (-) 357 WP_045510454.1 ribosome silencing factor -
  D2M30_RS13020 (D2M30_13040) yqeK 2578837..2579397 (-) 561 WP_014470705.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  D2M30_RS13025 (D2M30_13045) - 2579387..2579956 (-) 570 WP_045510448.1 nicotinate-nucleotide adenylyltransferase -
  D2M30_RS13030 (D2M30_13050) yhbY 2579967..2580257 (-) 291 WP_014470707.1 ribosome assembly RNA-binding protein YhbY -
  D2M30_RS13035 (D2M30_13055) aroE 2580251..2581093 (-) 843 WP_045510445.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22230.47 Da        Isoelectric Point: 5.5048

>NTDB_id=314036 D2M30_RS13000 WP_125122985.1 2576166..2576780(-) (comEA) [Bacillus amyloliquefaciens strain YP6]
MTKVMMTERQRQYLKILGVCLAAGAVFICILLFRPEKEEPVKQQSVLGQAASAPVTDKKDTADDKIMIDIKGAVRNPGVY
EMKAGDRVTQAIKKAGGLKEKADELNVNLAEQLQDGTIVYIPSEGEEANRPKTAAGEKENASVNINTASLDELQAIPGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=314036 D2M30_RS13000 WP_125122985.1 2576166..2576780(-) (comEA) [Bacillus amyloliquefaciens strain YP6]
ATGACGAAAGTGATGATGACGGAGCGGCAGAGGCAGTACCTGAAAATTCTTGGTGTTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTTGCTGTTTCGTCCCGAAAAAGAAGAGCCTGTCAAACAGCAGTCCGTCCTCGGCCAAGCAGCCTCAGCAC
CCGTCACGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATATTAAAGGAGCAGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTAAGAAAGCCGGCGGTCTGAAGGAAAAGGCGGATGAACTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATCGTTTATATCCCGAGTGAAGGCGAAGAGGCGAATCGCCCGAAAACCG
CAGCAGGTGAAAAGGAGAATGCTTCTGTCAATATCAATACGGCATCTCTTGACGAACTTCAGGCAATTCCCGGTGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAATGGGCGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGTTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

56

98.039

0.549
  comEA/celA/cilE Streptococcus pneumoniae R6

37.788

100

0.402
  comEA/celA/cilE Streptococcus pneumoniae Rx1

37.788

100

0.402
  comEA/celA/cilE Streptococcus pneumoniae D39

37.788

100

0.402
  comEA/celA/cilE Streptococcus mitis NCTC 12261

37.327

100

0.397
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

37.674

100

0.397
  comEA Lactococcus lactis subsp. cremoris KW2

35.268

100

0.387
  comEA/celA/cilE Streptococcus mitis SK321

35.814

100

0.377
  comEA Latilactobacillus sakei subsp. sakei 23K

32.479

100

0.373
  comEA Staphylococcus aureus MW2

34.389

100

0.373

Multiple sequence alignment