Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   D1115_RS01655 Genome accession   NZ_CP032093
Coordinates   321144..322823 (+) Length   559 a.a.
NCBI ID   WP_418369098.1    Uniprot ID   -
Organism   Vibrio alfacsensis strain CAIM 1831     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 316144..327823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1115_RS01635 (D1115_01640) pilM 318429..319440 (+) 1012 Protein_261 type IV pilus biogenesis protein PilM -
  D1115_RS01640 (D1115_01645) pilN 319424..319999 (+) 576 WP_128810017.1 PilN domain-containing protein Machinery gene
  D1115_RS01645 (D1115_01650) pilO 319992..320585 (+) 594 WP_128810018.1 type 4a pilus biogenesis protein PilO Machinery gene
  D1115_RS01650 (D1115_01655) pilP 320569..321084 (+) 516 WP_128812230.1 pilus assembly protein PilP Machinery gene
  D1115_RS01655 (D1115_01660) pilQ 321144..322823 (+) 1680 WP_418369098.1 type IV pilus secretin PilQ Machinery gene
  D1115_RS01660 (D1115_01665) aroK 323012..323530 (+) 519 WP_099080818.1 shikimate kinase AroK -
  D1115_RS01665 (D1115_01670) aroB 323559..324659 (+) 1101 WP_128810019.1 3-dehydroquinate synthase -
  D1115_RS01670 (D1115_01675) - 324711..325754 (+) 1044 Protein_268 ATP-binding protein -
  D1115_RS24135 - 325836..326225 (+) 390 Protein_269 SPOR domain-containing protein -
  D1115_RS01675 (D1115_01680) - 326295..327134 (+) 840 WP_128810021.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61518.21 Da        Isoelectric Point: 4.6087

>NTDB_id=313682 D1115_RS01655 WP_418369098.1 321144..322823(+) (pilQ) [Vibrio alfacsensis strain CAIM 1831]
MVIGFFSVWLFMFLASEGRAKASPPNELKSIDFRADNNTEAVIMIELTSSSVVVDIQRERDGLSIELLNTSVSDNQLHLL
DVRDFSTVIKQIDISRYHSATRLLAYTTDEYRYDYRLSGRWLEVTVVKEKSTKQAQSQNILKNKGRLISINFQDIPVRNV
LQLIADYNDFNLVVSDSVSGNLTLRLDDVPWPQVLDIILQVKGLDKRVDGNVILIAPLDELELREKQRLEKQKLEQEIGE
LSSEIIKVNFAKASDVAEMINGEGNISMLSERGSMTVDERTNSLLVRELPENIDVIREIIEALDIPVKQVQIEARIVTIN
EGNLDELGVRWGFTSINGSSTVGGSIENNLATLGLYDGGVGIDDFLNVNLAATNPSATSLAFQVAKLGAGTLLDLELSAL
QQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPGQVVK
TGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSLSNAVDKVPLLGDLPLLGALFRRSYEKVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=313682 D1115_RS01655 WP_418369098.1 321144..322823(+) (pilQ) [Vibrio alfacsensis strain CAIM 1831]
ATGGTGATTGGCTTTTTCTCTGTGTGGCTATTTATGTTTTTGGCATCAGAGGGGAGAGCAAAGGCGTCACCACCGAATGA
ATTAAAATCGATAGACTTCCGTGCAGATAACAATACAGAAGCGGTGATCATGATTGAGCTAACGTCATCGTCTGTCGTGG
TTGATATACAACGTGAAAGAGATGGGTTAAGTATTGAGCTGCTTAATACTTCGGTCAGTGATAACCAATTGCACCTTTTG
GACGTGAGAGATTTCTCTACCGTCATTAAGCAAATTGACATTTCTCGTTATCATTCGGCCACGCGTTTGTTGGCCTATAC
GACCGATGAATACCGCTATGACTATCGCCTGTCAGGACGCTGGTTAGAAGTGACAGTGGTGAAAGAAAAGTCAACTAAGC
AAGCACAAAGCCAGAACATTTTGAAGAATAAAGGGCGTTTAATCTCTATCAATTTTCAAGATATTCCGGTGCGAAATGTA
TTGCAGTTAATTGCTGACTATAACGATTTCAATCTCGTGGTATCGGATTCTGTGTCGGGTAATCTGACGTTACGCTTAGA
TGACGTACCTTGGCCACAAGTGTTAGATATCATTTTGCAAGTGAAAGGATTGGATAAACGGGTTGATGGCAATGTTATTT
TGATTGCCCCATTAGATGAACTGGAGCTAAGAGAAAAGCAGCGCCTTGAAAAGCAAAAGCTTGAGCAAGAAATAGGAGAG
CTTTCCTCTGAGATCATCAAAGTGAATTTTGCAAAAGCCTCGGACGTTGCGGAAATGATCAATGGCGAGGGTAATATCAG
CATGCTTTCTGAGAGGGGAAGTATGACCGTTGATGAGCGTACGAATTCATTGTTGGTGCGAGAATTACCAGAGAACATTG
ACGTTATTCGCGAAATTATAGAAGCGTTAGACATTCCCGTGAAACAAGTACAGATTGAAGCTCGCATTGTCACCATTAAT
GAGGGGAATCTCGATGAGCTTGGGGTACGCTGGGGTTTTACAAGTATAAATGGCAGTTCAACGGTTGGAGGCTCGATTGA
AAATAATCTCGCCACGCTTGGTTTGTATGATGGTGGCGTTGGCATTGATGATTTTCTTAATGTTAACCTTGCAGCGACCA
ACCCAAGTGCCACTAGCCTCGCTTTTCAAGTCGCAAAGTTAGGGGCAGGGACTTTACTTGATTTAGAGTTGTCTGCGTTG
CAGCAGGAATCGAAAGCAGAAATCATCTCAAGCCCACGCTTGATCACCACCAATAAGAAACCTGCTTATATTGAGCAAGG
AACGGAAATTCCGTATTTGGAGTCTTCATCAAGTGGGGCGACGTCGGTGACATTTAAAAAAGCAGTATTGAGTTTAAAAG
TGACACCGCAGATAACGCCAGATAATCGCTTAGTGTTGGATTTGAGCGTGACTCAAGACAGGCCAGGTCAAGTGGTGAAA
ACCGGCACGGGAGAAGCGGTTGCGATTGATACTCAGAGAATTGGCACTCAAGTTTTGGTCAATAATGGTGAAACGGTGGT
ATTGGGTGGTATTTTCCAGCATAGCCTCAGTAATGCCGTTGACAAAGTCCCTTTACTTGGTGATTTACCCTTGCTTGGCG
CGCTTTTTCGCCGAAGTTATGAAAAAGTAGGCAAAAGTGAGTTGTTGATTTTCGTGACGCCAAAGGTCGTAATTCAGTAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(170-217)

Bacterial type II/III secretion system short domain (Secretin_N)

(244-309)

Bacterial type II and III secretion system protein (Secretin)

(399-557)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

82.32

97.138

0.8
  pilQ Vibrio cholerae strain A1552

70.841

100

0.708
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

70.841

100

0.708
  pilQ Pseudomonas aeruginosa PAK

40.502

99.821

0.404
  pilQ Legionella pneumophila strain Lp02

40.775

96.959

0.395
  pilQ Legionella pneumophila strain ERS1305867

40.775

96.959

0.395
  comQ Acinetobacter baylyi ADP1

38.801

100

0.394
  pilQ Acinetobacter baumannii strain A118

36.082

100

0.376
  pilQ Acinetobacter baumannii D1279779

36.082

100

0.376

Multiple sequence alignment