Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CH631_RS03650 Genome accession   NZ_CP031680
Coordinates   722907..724298 (+) Length   463 a.a.
NCBI ID   WP_116939874.1    Uniprot ID   -
Organism   Haemophilus influenzae strain P672-7661     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 717907..729298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH631_RS03625 (CH631_03625) rsmE 717923..718660 (-) 738 WP_005656289.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CH631_RS03630 (CH631_03630) lnt 718710..720239 (-) 1530 WP_116940283.1 apolipoprotein N-acyltransferase -
  CH631_RS03635 (CH631_03635) corC 720262..721161 (-) 900 WP_116939870.1 CNNM family magnesium/cobalt transport protein CorC -
  CH631_RS03640 (CH631_03640) ampD 721792..722343 (-) 552 WP_071162800.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CH631_RS03645 (CH631_03645) pilA 722458..722910 (+) 453 WP_116939872.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CH631_RS03650 (CH631_03650) pilB 722907..724298 (+) 1392 WP_116939874.1 GspE/PulE family protein Machinery gene
  CH631_RS03655 (CH631_03655) pilC 724295..725512 (+) 1218 WP_112102453.1 type II secretion system F family protein Machinery gene
  CH631_RS03660 (CH631_03660) pilD 725509..726201 (+) 693 WP_112102454.1 prepilin peptidase Machinery gene
  CH631_RS03665 (CH631_03665) rho 726255..727517 (-) 1263 WP_112102455.1 transcription termination factor Rho -
  CH631_RS03670 (CH631_03670) metJ 727765..728082 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  CH631_RS03675 (CH631_03675) cueR 728096..728482 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  CH631_RS03680 (CH631_03680) - 728559..728765 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  CH631_RS03685 (CH631_03685) - 728839..729045 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52772.15 Da        Isoelectric Point: 5.9464

>NTDB_id=309854 CH631_RS03650 WP_116939874.1 722907..724298(+) (pilB) [Haemophilus influenzae strain P672-7661]
MTGYALLHTQRVTAQNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQHLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTESQQAFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINSQIGLDFSRLLRA
FLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCPKCG
GNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFQNLRVSGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=309854 CH631_RS03650 WP_116939874.1 722907..724298(+) (pilB) [Haemophilus influenzae strain P672-7661]
ATGACGGGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGGTATTTACGATCTCGCCTGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGCCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACACTTAATAAGCAAAATATTCGCTAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTCCGTCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGT
ATGACTGAAAGCCAACAGGCATTTCAACACGCGCTTAGCCAACCCCAAGGATTAATTTTAGTAACTGGCCCAACAGGAAG
TGGAAAAAGTATCTCACTTTACACTGCACTTCAATGGCTTAATACACCTGATAAACACATTATGACAGCGGAAGATCCCA
TTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATTCACAGATTGGATTAGATTTTAGCCGTCTATTGCGCGCT
TTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAAAGTGCAAGGATTGCACTACGTGCCGC
TCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGCTTACAACAACTCGGTA
TTCAGCAGTATGAAATTGAAAACAGTTTACTACTCGTCATTGCACAGCGTCTTGTACGAAAGCTTTGTCCAAAGTGCGGT
GGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCAACA
GAATGGCTATCAAACAGATTTTCAAAATTTACGTGTAAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGAAAG
AAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

97.198

100

0.974
  pilB Haemophilus influenzae Rd KW20

95.896

100

0.959
  pilB Glaesserella parasuis strain SC1401

57.675

98.488

0.568
  pilB Vibrio cholerae strain A1552

39.388

100

0.445
  pilB Legionella pneumophila strain ERS1305867

39.757

100

0.423
  pilB Vibrio campbellii strain DS40M4

40.329

100

0.423
  pilB Vibrio parahaemolyticus RIMD 2210633

40.289

100

0.421
  pilB Acinetobacter baylyi ADP1

38.508

100

0.413
  pilB Acinetobacter baumannii D1279779

39.085

100

0.406
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.607

100

0.38
  pilF Neisseria gonorrhoeae MS11

36.82

100

0.38
  pilF Thermus thermophilus HB27

36.421

100

0.374

Multiple sequence alignment