Detailed information NTG0309750

Bioinformatically predicted

Overview

• Name: pilC
• Type: Machinery gene
• Locus tag: DOM02_RS26995
• Genome accession: NZ_CP031677
• Coordinates: 5587956..5589173 (+)
• Length: 405 a.a.
• NCBI ID: WP_010792214.1
• Uniprot ID: A0A643EEA0
• Organism: Pseudomonas aeruginosa strain E80
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	5569148..5589173	5587956..5589173	within	0

Gene organization within MGE regions

Location: 5569148..5589173

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DOM02_RS26880 (DOM02_26760)	-	5569148..5569522 (+)	375	WP_023082014.1	hypothetical protein	-
DOM02_RS26885 (DOM02_26765)	-	5569522..5571075 (+)	1554	WP_023082015.1	DUF3631 domain-containing protein	-
DOM02_RS26890 (DOM02_26770)	-	5571075..5571368 (+)	294	WP_023082016.1	hypothetical protein	-
DOM02_RS26895 (DOM02_26775)	-	5571424..5572320 (+)	897	WP_023082017.1	hypothetical protein	-
DOM02_RS32865	-	5573244..5573408 (+)	165	WP_153519866.1	hypothetical protein	-
DOM02_RS26900 (DOM02_26785)	-	5573643..5574659 (+)	1017	WP_008435491.1	IS30 family transposase	-
DOM02_RS26905 (DOM02_26790)	-	5574742..5576118 (+)	1377	WP_278044744.1	anti-phage dCTP deaminase	-
DOM02_RS26910 (DOM02_26795)	-	5576840..5577346 (-)	507	WP_003096122.1	GNAT family N-acetyltransferase	-
DOM02_RS26920 (DOM02_26805)	-	5578086..5578367 (+)	282	WP_015649789.1	hypothetical protein	-
DOM02_RS32870	-	5578406..5578753 (-)	348	WP_153519867.1	hypothetical protein	-
DOM02_RS26930 (DOM02_26815)	-	5578810..5579091 (-)	282	WP_023082020.1	hypothetical protein	-
DOM02_RS26935	-	5579288..5579986 (+)	699	WP_023082021.1	hypothetical protein	-
DOM02_RS26940 (DOM02_26820)	-	5580011..5581015 (+)	1005	WP_023082022.1	pesticin C-terminus-like muramidase	-
DOM02_RS26945 (DOM02_26825)	-	5581028..5581690 (+)	663	WP_023082023.1	DUF3218 family protein	-
DOM02_RS26955 (DOM02_26835)	fliP	5581973..5582725 (+)	753	WP_023082025.1	flagellar type III secretion system pore protein FliP	-
DOM02_RS26960 (DOM02_26840)	fliQ	5582770..5583039 (+)	270	WP_023081841.1	flagellar biosynthesis protein FliQ	-
DOM02_RS26965 (DOM02_26845)	fliR	5583039..5583815 (+)	777	WP_003083054.1	flagellar biosynthetic protein FliR	-
DOM02_RS26970 (DOM02_26850)	-	5583818..5584077 (+)	260	Protein_5206	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
DOM02_RS26975 (DOM02_26855)	-	5585075..5585317 (+)	243	WP_023082027.1	hypothetical protein	-
DOM02_RS26985 (DOM02_26865)	-	5585548..5586021 (-)	474	WP_015503553.1	pilin	-
DOM02_RS26990 (DOM02_26870)	pilB	5586252..5587952 (+)	1701	WP_003120945.1	type IV-A pilus assembly ATPase PilB	Machinery gene
DOM02_RS26995 (DOM02_26875)	pilC	5587956..5589173 (+)	1218	WP_010792214.1	type II secretion system F family protein	Machinery gene

Sequence

Protein

• Length: 405 a.a.
• Molecular weight: 44242.19 Da
• Isoelectric Point: 9.8988

>NTDB_id=309750 DOM02_RS26995 WP_010792214.1 5587956..5589173(+) (pilC) [Pseudomonas aeruginosa strain E80]
MADKALKTCVFVWEGTDKKGAKVKGELAGQNTMLVKAQLRKQGINPLKVRKKGITLLGKGKRVKPMDIALFTRQMATMMG
AGVPLLQSFDIISEGFDNPNMRKLVDEIKQEVSAGNSLANSLRKKPLYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVVLVAIIVSAILLIKVVPQFQSVFSSFGAELPAFTMMVINLSNLLQEWWLVVLIGLFSASFAIK
ESHKRSVNFRNTVDRYMLKIPIIGGILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRNAVSKIKQDVSTGM
QLNFSMRTTNVFPNMAIQMTAIGEESGSLDDMLGKVAAFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide

• Length: 1218 bp

>NTDB_id=309750 DOM02_RS26995 WP_010792214.1 5587956..5589173(+) (pilC) [Pseudomonas aeruginosa strain E80]
ATGGCGGATAAAGCGTTAAAAACCTGTGTTTTCGTCTGGGAAGGCACGGACAAGAAAGGGGCAAAGGTCAAGGGGGAGTT
GGCTGGCCAGAACACTATGCTGGTGAAGGCGCAACTGCGCAAACAGGGCATAAATCCGCTCAAGGTCAGAAAAAAGGGCA
TTACCCTTCTCGGCAAGGGAAAGCGCGTAAAGCCGATGGACATCGCACTATTTACTCGTCAGATGGCAACCATGATGGGT
GCTGGGGTTCCTCTCTTGCAATCGTTCGACATAATTTCCGAAGGTTTCGACAATCCCAACATGCGTAAGCTGGTCGACGA
GATCAAACAAGAGGTCTCGGCGGGTAACAGCCTGGCAAACTCCTTGCGAAAGAAACCTCTTTATTTTGACGATCTCTATT
GCAACCTGGTGGATGCAGGTGAACAATCTGGCGCCTTGGAGACTTTGCTTGACCGTGTTGCCACCTATAAGGAAAAAACC
GAATCGCTAAAAGCCAAGATAAAAAAGGCTATGACATACCCCATTGCCGTCGTCTTGGTCGCGATCATTGTTTCAGCCAT
TCTCCTGATCAAGGTCGTACCCCAGTTCCAATCCGTTTTCTCCAGTTTCGGAGCGGAACTACCAGCCTTTACAATGATGG
TGATAAACCTGTCAAATCTTTTACAGGAATGGTGGCTAGTCGTCCTCATCGGATTATTCAGCGCCAGCTTCGCCATCAAG
GAATCGCATAAGCGTTCAGTAAATTTCCGTAACACGGTAGACCGCTATATGTTGAAAATACCGATAATCGGAGGAATACT
TTACAAATCAGCCGTGGCACGTTACGCCCGTACACTTTCAACAACTTTCGCCGCAGGTGTTCCATTGGTGGAGGCACTAG
ACTCTGTCTCTGGAGCGACAGGAAACGTGGTATTCCGCAACGCGGTAAGCAAGATCAAACAGGATGTATCCACAGGTATG
CAATTGAACTTCTCCATGAGAACGACCAATGTATTCCCCAACATGGCAATCCAGATGACAGCCATCGGTGAGGAATCCGG
TTCTCTGGACGACATGTTGGGCAAGGTGGCTGCGTTCTATGAGGAGGAAGTAGACAATGCCGTCGACAATCTGACAACTC
TCATGGAACCTATGATCATGGCCGTGCTCGGCGTACTGGTCGGCGGCCTGATCATCGCTATGTATCTACCGATCTTCCAA
CTGGGTTCCGTTGTGTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (276-397)

• Type II secretion system (T2SS), protein F (T2SSF) (71-194)

3D structure

• AlphaFold DB: A0A643EEA0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilC	Pseudomonas stutzeri DSM 10701	78.765	100	0.788
pilC	Acinetobacter baumannii D1279779	58.911	99.753	0.588
pilC	Acinetobacter baylyi ADP1	59.045	98.272	0.58
pilC	Legionella pneumophila strain ERS1305867	54.293	97.778	0.531
pilG	Neisseria meningitidis 44/76-A	44.03	99.259	0.437
pilG	Neisseria gonorrhoeae MS11	43.781	99.259	0.435
pilC	Vibrio cholerae strain A1552	43.21	100	0.432
pilC	Vibrio campbellii strain DS40M4	43.182	97.778	0.422
pilC	Thermus thermophilus HB27	37.406	99.012	0.37