Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DOM02_RS26990 Genome accession   NZ_CP031677
Coordinates   5586252..5587952 (+) Length   566 a.a.
NCBI ID   WP_003120945.1    Uniprot ID   A0A1L7HA63
Organism   Pseudomonas aeruginosa strain E80     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5569148..5589173 5586252..5587952 within 0


Gene organization within MGE regions


Location: 5569148..5589173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOM02_RS26880 (DOM02_26760) - 5569148..5569522 (+) 375 WP_023082014.1 hypothetical protein -
  DOM02_RS26885 (DOM02_26765) - 5569522..5571075 (+) 1554 WP_023082015.1 DUF3631 domain-containing protein -
  DOM02_RS26890 (DOM02_26770) - 5571075..5571368 (+) 294 WP_023082016.1 hypothetical protein -
  DOM02_RS26895 (DOM02_26775) - 5571424..5572320 (+) 897 WP_023082017.1 hypothetical protein -
  DOM02_RS32865 - 5573244..5573408 (+) 165 WP_153519866.1 hypothetical protein -
  DOM02_RS26900 (DOM02_26785) - 5573643..5574659 (+) 1017 WP_008435491.1 IS30 family transposase -
  DOM02_RS26905 (DOM02_26790) - 5574742..5576118 (+) 1377 WP_278044744.1 anti-phage dCTP deaminase -
  DOM02_RS26910 (DOM02_26795) - 5576840..5577346 (-) 507 WP_003096122.1 GNAT family N-acetyltransferase -
  DOM02_RS26920 (DOM02_26805) - 5578086..5578367 (+) 282 WP_015649789.1 hypothetical protein -
  DOM02_RS32870 - 5578406..5578753 (-) 348 WP_153519867.1 hypothetical protein -
  DOM02_RS26930 (DOM02_26815) - 5578810..5579091 (-) 282 WP_023082020.1 hypothetical protein -
  DOM02_RS26935 - 5579288..5579986 (+) 699 WP_023082021.1 hypothetical protein -
  DOM02_RS26940 (DOM02_26820) - 5580011..5581015 (+) 1005 WP_023082022.1 pesticin C-terminus-like muramidase -
  DOM02_RS26945 (DOM02_26825) - 5581028..5581690 (+) 663 WP_023082023.1 DUF3218 family protein -
  DOM02_RS26955 (DOM02_26835) fliP 5581973..5582725 (+) 753 WP_023082025.1 flagellar type III secretion system pore protein FliP -
  DOM02_RS26960 (DOM02_26840) fliQ 5582770..5583039 (+) 270 WP_023081841.1 flagellar biosynthesis protein FliQ -
  DOM02_RS26965 (DOM02_26845) fliR 5583039..5583815 (+) 777 WP_003083054.1 flagellar biosynthetic protein FliR -
  DOM02_RS26970 (DOM02_26850) - 5583818..5584077 (+) 260 Protein_5206 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
  DOM02_RS26975 (DOM02_26855) - 5585075..5585317 (+) 243 WP_023082027.1 hypothetical protein -
  DOM02_RS26985 (DOM02_26865) - 5585548..5586021 (-) 474 WP_015503553.1 pilin -
  DOM02_RS26990 (DOM02_26870) pilB 5586252..5587952 (+) 1701 WP_003120945.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DOM02_RS26995 (DOM02_26875) pilC 5587956..5589173 (+) 1218 WP_010792214.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62385.25 Da        Isoelectric Point: 5.7055

>NTDB_id=309749 DOM02_RS26990 WP_003120945.1 5586252..5587952(+) (pilB) [Pseudomonas aeruginosa strain E80]
MNDSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSAEKSTQEDTSAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=309749 DOM02_RS26990 WP_003120945.1 5586252..5587952(+) (pilB) [Pseudomonas aeruginosa strain E80]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTATCCGCCGAGCAGTTCGGTATCGCCTACTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAGCACCGGGTCATCCCTTTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGATCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAACTGTTCGAAAGCGCTACCGATGGTCTCGCAGGCCTCGACGATGTTGACCTCGAG
GGACTGGATATCGGTAGCGCGGAAAAGTCCACCCAGGAGGATACCAGCGCAGAAGCGGACGACGCTCCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATAAAGGGTGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGACATTTCCGAACGGCGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGAGCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAAATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGACATATGGTGATGTCCACCCTACACACCAACAGCGCCGCCGA
GACCCTGACTCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTTCCGGAA
GACAAGATCGGTACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1L7HA63

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.692

100

0.583
  pilB Acinetobacter baylyi ADP1

57.371

99.47

0.571
  pilB Legionella pneumophila strain ERS1305867

55.124

100

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

51.064

99.647

0.509
  pilF Neisseria gonorrhoeae MS11

50.712

99.293

0.504
  pilB Vibrio cholerae strain A1552

50.265

99.823

0.502
  pilB Vibrio campbellii strain DS40M4

49.823

99.647

0.496
  pilF Thermus thermophilus HB27

39.572

99.117

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.607

93.11

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

38.563

93.463

0.36

Multiple sequence alignment