Detailed information NTG0307363

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: L3107_RS08935
• Genome accession: NZ_CP031538
• Coordinates: 1755064..1755645 (-)
• Length: 193 a.a.
• NCBI ID: WP_231097919.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain 3107
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1750064..1760645

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L3107_RS08910 (L3107_1743)	-	1750408..1750623 (-)	216	WP_014572212.1	F0F1 ATP synthase subunit C	-
L3107_RS08915 (L3107_1744)	-	1750806..1751582 (-)	777	WP_021165645.1	alpha/beta hydrolase family protein	-
L3107_RS08920 (L3107_1745)	-	1751866..1752171 (-)	306	Protein_1744	DNA internalization-related competence protein ComEC/Rec2	-
L3107_RS08925 (L3107_1746)	-	1752192..1753082 (-)	891	WP_064305213.1	IS982 family transposase	-
L3107_RS13790 (L3107_1747)	comEC	1753159..1753668 (-)	510	WP_021165649.1	MBL fold metallo-hydrolase	Machinery gene
L3107_RS08930 (L3107_1748)	comEC	1753665..1754936 (-)	1272	WP_324187043.1	ComEC/Rec2 family competence protein	Machinery gene
L3107_RS08935 (L3107_1749)	comEA	1755064..1755645 (-)	582	WP_231097919.1	helix-hairpin-helix domain-containing protein	Machinery gene
L3107_RS08940 (L3107_1750)	-	1755776..1757137 (-)	1362	WP_014572203.1	ABC transporter permease	-
L3107_RS08945 (L3107_1751)	-	1757134..1758066 (-)	933	WP_014572202.1	ABC transporter ATP-binding protein	-
L3107_RS08950 (L3107_1752)	-	1758172..1758570 (-)	399	WP_011676775.1	hypothetical protein	-
L3107_RS08955 (L3107_1753)	-	1758687..1759247 (-)	561	WP_021165651.1	GNAT family N-acetyltransferase	-
L3107_RS08960 (L3107_1754)	-	1759420..1760598 (-)	1179	WP_011676777.1	SLC13 family permease	-

Sequence

Protein

• Length: 193 a.a.
• Molecular weight: 21052.51 Da
• Isoelectric Point: 4.8079

>NTDB_id=307363 L3107_RS08935 WP_231097919.1 1755064..1755645(-) (comEA) [Lactococcus cremoris strain 3107]
MEFLYIFTNSPKQAENLSVENLSSSSTKSSVSKFNSSSEKNKNEIMVDLKGAVAKPNVYQISSDERLVDLIRQAGGFTDQ
ADQKSINLSAKLKDEEVIYVPKLGESSSSESTDSPISSSVSNQVSTTSGPKININKADLTELQKLTGIGQKKAQDIIDFR
MKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 582 bp

>NTDB_id=307363 L3107_RS08935 WP_231097919.1 1755064..1755645(-) (comEA) [Lactococcus cremoris strain 3107]
GTGGAATTTTTATATATTTTCACCAATTCACCAAAGCAAGCTGAAAATCTGTCAGTAGAAAATTTGAGCTCTAGTTCTAC
CAAAAGCTCTGTCAGTAAATTTAATAGCAGCAGTGAAAAAAATAAAAACGAAATTATGGTTGATTTGAAAGGAGCGGTTG
CAAAACCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAGGCAAGCGGGAGGATTTACTGACCAA
GCAGACCAAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTGATTTATGTACCAAAACTTGGGGAAAGTTC
AAGTTCAGAAAGTACTGACAGTCCTATTAGCAGCTCTGTCAGTAATCAAGTTTCAACGACAAGTGGTCCAAAAATAAATA
TCAATAAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAAAGCGCAAGATATTATTGATTTTCGC
ATGAAAAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTGGGGATAAAACATTAGAAAAATTGAA
AGATGAGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (46-101)

• Helix-hairpin-helix motif (HHH_3) (129-190)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	94.792	99.482	0.943
comEA/celA/cilE	Streptococcus mitis SK321	45.213	97.409	0.44
comEA	Latilactobacillus sakei subsp. sakei 23K	42.781	96.891	0.415
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	48.993	77.202	0.378
comEA/celA/cilE	Streptococcus pneumoniae R6	48.322	77.202	0.373
comEA/celA/cilE	Streptococcus pneumoniae Rx1	48.322	77.202	0.373
comEA/celA/cilE	Streptococcus pneumoniae D39	48.322	77.202	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	47.651	77.202	0.368
comEA	Staphylococcus aureus MW2	40	90.674	0.363
comEA	Streptococcus thermophilus LMD-9	43.478	83.42	0.363