Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DXX82_RS07245 Genome accession   NZ_CP031477
Coordinates   1477563..1478942 (-) Length   459 a.a.
NCBI ID   WP_115912282.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M27794     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1472563..1483942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXX82_RS07210 mreD 1472595..1473083 (+) 489 WP_005695501.1 rod shape-determining protein MreD -
  DXX82_RS07215 rhlB 1473124..1474383 (-) 1260 WP_115912279.1 ATP-dependent RNA helicase RhlB -
  DXX82_RS07220 - 1474473..1474754 (-) 282 WP_032822874.1 GNAT family N-acetyltransferase -
  DXX82_RS07225 yacG 1474751..1474957 (-) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  DXX82_RS07230 coaE 1474947..1475570 (-) 624 WP_005695506.1 dephospho-CoA kinase -
  DXX82_RS07235 pilD 1475657..1476334 (-) 678 WP_049367123.1 A24 family peptidase Machinery gene
  DXX82_RS07240 pilC 1476331..1477554 (-) 1224 WP_049376124.1 type II secretion system F family protein Machinery gene
  DXX82_RS07245 pilB 1477563..1478942 (-) 1380 WP_115912282.1 GspE/PulE family protein Machinery gene
  DXX82_RS07250 pilA 1478949..1479392 (-) 444 WP_005697917.1 prepilin peptidase-dependent pilin Machinery gene
  DXX82_RS07255 ampD 1479535..1480098 (+) 564 WP_005695512.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DXX82_RS07265 rppH 1480583..1481176 (+) 594 WP_049376123.1 RNA pyrophosphohydrolase -
  DXX82_RS07270 - 1481176..1481970 (+) 795 WP_005695514.1 sulfite exporter TauE/SafE family protein -
  DXX82_RS07275 lgt 1481979..1482788 (+) 810 WP_115912284.1 prolipoprotein diacylglyceryl transferase -
  DXX82_RS07280 - 1482799..1483650 (+) 852 WP_115912286.1 thymidylate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52229.66 Da        Isoelectric Point: 6.5550

>NTDB_id=307140 DXX82_RS07245 WP_115912282.1 1477563..1478942(-) (pilB) [Haemophilus parainfluenzae strain M27794]
MAYQAIAKNGEIYHISPQYWQQNQQQQALLLRYFTLPLKEDEHHLWLAVDSLNNLAACETFAFLSGKLVEPILFETTQLK
QLLQSLAPKANQIEYQTTFYHHSEDENAANLSNEDEPVIQLLNSIFETALQKNASDIHLETQPSGLLIRLRIDGVLQPQP
VISKAFANRVISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKAVLRLQQNKPVQLSFAELGMTQK
QQIQFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPVEIELEGIIQTQVNPQIGLDFSRLLRTFLR
QDPDIIMLGEIRDEESALMALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQSHEIENSLLLVIAQRLVRKRCLKCGQHF
SDSCDCHQGYRGRIGVYQFLQCKNNRYQTDFDSLYQSALEKVKDHITDLDEIQRVLGKK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=307140 DXX82_RS07245 WP_115912282.1 1477563..1478942(-) (pilB) [Haemophilus parainfluenzae strain M27794]
ATGGCCTATCAAGCAATCGCAAAAAATGGCGAGATTTATCATATCTCGCCACAATATTGGCAACAAAACCAACAGCAACA
AGCTTTACTGTTGCGTTACTTTACGCTCCCTCTTAAAGAAGACGAACACCATTTATGGCTTGCGGTGGATTCGCTAAATA
ATCTTGCAGCTTGTGAAACCTTTGCTTTTTTAAGTGGAAAATTAGTTGAACCGATTTTATTTGAAACGACTCAACTCAAA
CAACTTTTACAATCCCTTGCCCCTAAAGCCAATCAAATAGAATACCAAACAACGTTTTATCATCATTCTGAAGACGAAAA
CGCTGCTAATTTATCTAATGAAGATGAACCCGTTATTCAATTATTAAATAGTATTTTTGAAACAGCCCTACAAAAAAATG
CCTCCGATATTCATTTAGAAACTCAGCCAAGTGGACTCTTAATTCGTTTACGTATTGATGGCGTACTACAGCCACAGCCG
GTAATCAGTAAAGCGTTCGCCAATCGTGTGATTTCTCGTTTAAAACTCTTAGCTAAACTGGATATCAGTGAAACTCGGCT
ACCACAAGATGGCCGCTTTCAATTCAAAACCACGTTCTCCGATATTTTAGATTTCCGCCTTTCTACCTTGCCCACACATT
GGGGAGAAAAAGCGGTATTACGATTACAACAAAATAAACCAGTTCAGTTAAGTTTTGCTGAACTTGGCATGACACAAAAG
CAACAAATCCAATTTCAACACGCGCTCAGCCAACCTCAAGGTTTAATTCTTGTAACTGGACCAACAGGAAGCGGAAAAAG
TATCTCGCTTTATACCGCACTTCAATGGCTCAATACGCCAGATAAACATATTATGACAGCAGAAGATCCCGTTGAAATTG
AATTAGAAGGTATTATTCAAACTCAAGTGAATCCGCAAATCGGTTTAGATTTTAGCCGATTACTTCGTACATTTTTACGA
CAAGATCCCGACATTATTATGCTTGGTGAAATTCGAGATGAAGAAAGTGCATTGATGGCATTAAGAGCCGCTCAAACCGG
CCATTTAGTGCTTTCTACCTTACATACCAACGATGCCATCTCAGCGATTTCACGCTTGCAACAACTAGGCATTCAATCTC
ACGAAATTGAAAACAGTCTCTTATTAGTTATCGCACAACGATTGGTGCGTAAACGTTGCTTAAAGTGCGGTCAACATTTC
AGTGATTCTTGCGATTGCCATCAGGGATACCGAGGTCGAATTGGGGTATATCAATTTTTACAATGCAAAAACAATCGCTA
CCAAACTGATTTCGATTCACTTTATCAAAGCGCGTTAGAAAAAGTAAAAGATCACATCACCGATCTCGATGAGATTCAAC
GCGTGTTAGGCAAAAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(124-391)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-88)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

77.876

98.475

0.767
  pilB Haemophilus influenzae Rd KW20

77.212

98.475

0.76
  pilB Glaesserella parasuis strain SC1401

59.071

98.475

0.582
  pilB Vibrio campbellii strain DS40M4

40.787

100

0.429
  pilB Vibrio cholerae strain A1552

40.496

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.292

100

0.42
  pilB Legionella pneumophila strain ERS1305867

38.571

100

0.412
  pilB Acinetobacter baylyi ADP1

38.289

100

0.41
  pilB Acinetobacter baumannii D1279779

38.589

100

0.405
  pilF Neisseria gonorrhoeae MS11

36.975

100

0.383
  pilF Thermus thermophilus HB27

36.575

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.307

100

0.364

Multiple sequence alignment