Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DXX82_RS07250 Genome accession   NZ_CP031477
Coordinates   1478949..1479392 (-) Length   147 a.a.
NCBI ID   WP_005697917.1    Uniprot ID   A0A369YN52
Organism   Haemophilus parainfluenzae strain M27794     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1473949..1484392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXX82_RS07220 - 1474473..1474754 (-) 282 WP_032822874.1 GNAT family N-acetyltransferase -
  DXX82_RS07225 yacG 1474751..1474957 (-) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  DXX82_RS07230 coaE 1474947..1475570 (-) 624 WP_005695506.1 dephospho-CoA kinase -
  DXX82_RS07235 pilD 1475657..1476334 (-) 678 WP_049367123.1 A24 family peptidase Machinery gene
  DXX82_RS07240 pilC 1476331..1477554 (-) 1224 WP_049376124.1 type II secretion system F family protein Machinery gene
  DXX82_RS07245 pilB 1477563..1478942 (-) 1380 WP_115912282.1 GspE/PulE family protein Machinery gene
  DXX82_RS07250 pilA 1478949..1479392 (-) 444 WP_005697917.1 prepilin peptidase-dependent pilin Machinery gene
  DXX82_RS07255 ampD 1479535..1480098 (+) 564 WP_005695512.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DXX82_RS07265 rppH 1480583..1481176 (+) 594 WP_049376123.1 RNA pyrophosphohydrolase -
  DXX82_RS07270 - 1481176..1481970 (+) 795 WP_005695514.1 sulfite exporter TauE/SafE family protein -
  DXX82_RS07275 lgt 1481979..1482788 (+) 810 WP_115912284.1 prolipoprotein diacylglyceryl transferase -
  DXX82_RS07280 - 1482799..1483650 (+) 852 WP_115912286.1 thymidylate synthase -
  DXX82_RS07285 tadA 1483687..1484178 (+) 492 WP_240320516.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15151.42 Da        Isoelectric Point: 8.4674

>NTDB_id=307141 DXX82_RS07250 WP_005697917.1 1478949..1479392(-) (pilA) [Haemophilus parainfluenzae strain M27794]
MKLTFSKPLHKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAISELLQASAPYKSDVELCVYSTNAPTNCSGGSNGIA
ADITTAKGYVKSITTKSGVITVTGNGALDGISYSLTATGSTSSGVTWTTACPSNADLFPAGFCSPTK

Nucleotide


Download         Length: 444 bp        

>NTDB_id=307141 DXX82_RS07250 WP_005697917.1 1478949..1479392(-) (pilA) [Haemophilus parainfluenzae strain M27794]
ATGAAACTGACTTTTTCTAAACCTTTACATAAAGGTTTTACGTTAATTGAATTGATGATCGTGATTGCGATTATTGCTAT
TCTCGCGACGATCGCCATTCCGTCTTATCAAAATTATACCAAAAAAGCGGCTATCTCCGAATTGTTACAAGCTTCGGCAC
CTTATAAATCTGATGTGGAGTTATGTGTCTATAGCACCAATGCCCCGACAAACTGTTCTGGTGGTTCAAATGGCATTGCA
GCAGACATCACTACTGCAAAAGGTTATGTTAAATCCATTACCACGAAATCGGGTGTGATTACGGTAACAGGAAATGGTGC
ATTAGATGGGATCAGTTATTCTTTAACGGCGACAGGCTCAACCTCATCAGGTGTTACCTGGACAACCGCTTGCCCAAGCA
ATGCGGATTTATTCCCTGCGGGTTTCTGCTCTCCTACCAAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A369YN52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

75.694

97.959

0.741
  pilA Haemophilus influenzae Rd KW20

73.611

97.959

0.721
  pilA Glaesserella parasuis strain SC1401

56.081

100

0.565
  pilA Vibrio campbellii strain DS40M4

41.096

99.32

0.408
  pilE Neisseria gonorrhoeae MS11

41.985

89.116

0.374
  pilA2 Legionella pneumophila str. Paris

42.188

87.075

0.367

Multiple sequence alignment