Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   DV445_RS02710 Genome accession   NZ_CP031255
Coordinates   539216..540439 (+) Length   407 a.a.
NCBI ID   WP_049248630.1    Uniprot ID   A0A378TZM3
Organism   Neisseria elongata strain M15910     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 537512..552081 539216..540439 within 0


Gene organization within MGE regions


Location: 537512..552081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV445_RS02705 pilF 537512..539191 (+) 1680 WP_107853523.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DV445_RS02710 pilG 539216..540439 (+) 1224 WP_049248630.1 type II secretion system F family protein Machinery gene
  DV445_RS02715 pilD 540450..541322 (+) 873 WP_107853522.1 prepilin peptidase Machinery gene
  DV445_RS02720 coaE 541322..541924 (+) 603 WP_114921596.1 dephospho-CoA kinase -
  DV445_RS02725 zapD 541939..542700 (+) 762 WP_114921597.1 cell division protein ZapD -
  DV445_RS02730 - 542697..542903 (+) 207 WP_107853519.1 DNA gyrase inhibitor YacG -
  DV445_RS02735 rpmE 543035..543253 (-) 219 WP_049248635.1 50S ribosomal protein L31 -
  DV445_RS02740 - 543388..543606 (-) 219 WP_107853518.1 SlyX family protein -
  DV445_RS02745 - 543607..544989 (-) 1383 WP_107853517.1 Na+/H+ antiporter family protein -
  DV445_RS02750 - 545282..546256 (-) 975 WP_234404629.1 hypothetical protein -
  DV445_RS02755 - 546424..547749 (+) 1326 WP_114921598.1 M23 family metallopeptidase -
  DV445_RS02760 - 548044..552081 (-) 4038 WP_162817619.1 pilus assembly protein -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44639.73 Da        Isoelectric Point: 9.8738

>NTDB_id=305121 DV445_RS02710 WP_049248630.1 539216..540439(+) (pilG) [Neisseria elongata strain M15910]
MKINKAKNEKGHRFTFEGKHTQSEQIVRGEVVATNEDEAKKKLLRRGIKPTRVTKVKATRKKRITQADITVFTRQLATMM
KAGLPLMQAFEIVARGHVNPSMTQLLMAIRSDVEQGSSMGAAFAKHPKYFDKFYCNLISAGEAGGVLEHLLDKLAVYKEK
TQSIKKKVKSALTYPIAVVAVAVILVIVMMMYVLPAFGKIYKDMGAELPGLTQMLMSASDFFVEYGWIVIIVLIGLVVGF
VHLHRNSPELQKRVDMLVLKMPIFGDIVRKATIARWARTTASLFAAGVPLVEALDSVAGASGNILYEEATISIKSQVNQG
MSLTSGMQNTGLFPNMVLQMAAIGEESGSLDDMLNKAAEFYEEEVDTAVSQLSSLMEPIIMVVLGSIIGTMLVAMYLPLF
NIGNVIG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=305121 DV445_RS02710 WP_049248630.1 539216..540439(+) (pilG) [Neisseria elongata strain M15910]
ATGAAGATAAATAAAGCAAAAAATGAAAAAGGGCATCGTTTTACTTTTGAAGGGAAACATACACAAAGTGAACAGATTGT
ACGTGGTGAAGTAGTTGCGACTAATGAGGATGAGGCGAAGAAAAAATTATTACGTAGAGGAATTAAACCTACTCGTGTGA
CTAAAGTAAAAGCCACCCGTAAGAAGCGAATTACTCAAGCAGATATTACGGTTTTTACTCGACAACTCGCTACTATGATG
AAGGCTGGATTGCCTTTAATGCAAGCTTTTGAGATTGTTGCTAGAGGGCATGTTAATCCTTCGATGACTCAGTTGTTGAT
GGCTATACGCAGCGATGTGGAGCAGGGGTCTTCGATGGGGGCTGCATTTGCAAAGCATCCCAAGTATTTCGATAAATTTT
ATTGTAACTTAATTTCTGCAGGTGAAGCTGGTGGTGTGTTAGAACATTTGTTAGATAAGCTCGCTGTATACAAAGAAAAA
ACTCAGTCTATCAAGAAAAAAGTTAAATCAGCTCTTACTTATCCTATAGCCGTTGTTGCAGTGGCAGTTATATTAGTTAT
TGTAATGATGATGTATGTATTGCCTGCATTTGGTAAGATTTATAAAGATATGGGTGCTGAGTTGCCAGGATTGACACAAA
TGTTAATGAGTGCGTCAGATTTTTTTGTTGAATATGGCTGGATTGTAATTATTGTATTAATAGGGTTAGTTGTTGGATTT
GTTCATTTACATCGAAATTCACCTGAGCTGCAGAAAAGGGTAGATATGCTAGTACTTAAAATGCCAATCTTCGGTGATAT
TGTACGAAAGGCCACTATTGCTAGATGGGCCCGGACGACAGCTAGTTTGTTTGCTGCAGGTGTTCCTTTAGTTGAGGCAT
TGGATTCAGTTGCTGGTGCTTCAGGTAATATTTTATATGAAGAGGCTACTATTTCAATTAAATCACAGGTAAATCAAGGT
ATGTCATTAACATCGGGGATGCAGAATACTGGTCTTTTCCCTAATATGGTCCTACAAATGGCAGCTATTGGTGAAGAGTC
TGGATCATTGGATGATATGCTAAATAAAGCAGCTGAATTTTATGAGGAAGAGGTAGATACTGCAGTTTCTCAATTATCCT
CTTTGATGGAACCTATCATAATGGTGGTTCTAGGAAGTATTATTGGTACTATGTTGGTTGCTATGTATTTACCTCTGTTC
AATATAGGCAATGTTATCGGGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378TZM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

68.08

98.526

0.671
  pilG Neisseria meningitidis 44/76-A

67.332

98.526

0.663
  pilC Legionella pneumophila strain ERS1305867

47.607

97.543

0.464
  pilC Pseudomonas stutzeri DSM 10701

46.25

98.28

0.455
  pilC Acinetobacter baumannii D1279779

45.226

97.789

0.442
  pilC Acinetobacter baylyi ADP1

44.472

97.789

0.435
  pilC Vibrio cholerae strain A1552

38.191

97.789

0.373
  pilC Vibrio campbellii strain DS40M4

36.543

99.509

0.364

Multiple sequence alignment