Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DV445_RS02705 Genome accession   NZ_CP031255
Coordinates   537512..539191 (+) Length   559 a.a.
NCBI ID   WP_107853523.1    Uniprot ID   -
Organism   Neisseria elongata strain M15910     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 537512..552081 537512..539191 within 0


Gene organization within MGE regions


Location: 537512..552081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV445_RS02705 pilF 537512..539191 (+) 1680 WP_107853523.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DV445_RS02710 pilG 539216..540439 (+) 1224 WP_049248630.1 type II secretion system F family protein Machinery gene
  DV445_RS02715 pilD 540450..541322 (+) 873 WP_107853522.1 prepilin peptidase Machinery gene
  DV445_RS02720 coaE 541322..541924 (+) 603 WP_114921596.1 dephospho-CoA kinase -
  DV445_RS02725 zapD 541939..542700 (+) 762 WP_114921597.1 cell division protein ZapD -
  DV445_RS02730 - 542697..542903 (+) 207 WP_107853519.1 DNA gyrase inhibitor YacG -
  DV445_RS02735 rpmE 543035..543253 (-) 219 WP_049248635.1 50S ribosomal protein L31 -
  DV445_RS02740 - 543388..543606 (-) 219 WP_107853518.1 SlyX family protein -
  DV445_RS02745 - 543607..544989 (-) 1383 WP_107853517.1 Na+/H+ antiporter family protein -
  DV445_RS02750 - 545282..546256 (-) 975 WP_234404629.1 hypothetical protein -
  DV445_RS02755 - 546424..547749 (+) 1326 WP_114921598.1 M23 family metallopeptidase -
  DV445_RS02760 - 548044..552081 (-) 4038 WP_162817619.1 pilus assembly protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62193.52 Da        Isoelectric Point: 5.8712

>NTDB_id=305120 DV445_RS02705 WP_107853523.1 537512..539191(+) (pilF) [Neisseria elongata strain M15910]
MSMGLLRVLVQNKTLSSEQAGRYQQALKDNKDIIAMLFADGITNPKALAELLAGMFNYPVLDLNYYPRSNIVQDVLNEEQ
MQANACVPIFKRGNKIFLAVSDPTRIQEFQKVVLSSGFSIDLVIVRHDQLMTLVEWLGQRSTTILREMSQDQLDVGASQS
LYIDNEEAEDGPIARFIHKTLSDALNAGASDIHFEFYEHMARVRFRVDGQLREVVQPPVAIRGQLASRIKVMARLDISEK
RIPQDGRIQIAFSKHGRPTDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEDFQKELLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTEGVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAS
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKEPMERPPEHALRKAGFSDEDLAKDWTM
YRAVGCDTCRGKGYKGRAGVYEVMPITDEMQRVIMEGGTEVDIQKMAYQEGMVDLRRAGLLKVMSGITSLEEVLAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=305120 DV445_RS02705 WP_107853523.1 537512..539191(+) (pilF) [Neisseria elongata strain M15910]
ATGAGTATGGGATTATTAAGGGTTTTGGTTCAGAATAAAACCCTCAGCAGTGAACAAGCTGGACGCTATCAACAAGCATT
GAAAGATAATAAAGATATTATTGCTATGTTGTTTGCTGACGGGATAACTAATCCCAAAGCATTGGCAGAATTGTTGGCTG
GGATGTTCAATTACCCTGTTCTTGATTTGAATTACTATCCGCGTAGCAATATCGTTCAGGATGTTTTAAACGAGGAGCAA
ATGCAGGCTAATGCGTGTGTTCCTATATTTAAACGTGGGAATAAGATATTCCTTGCAGTATCAGATCCAACAAGAATTCA
AGAATTTCAAAAAGTCGTTCTTTCTTCCGGATTTTCAATAGATTTGGTGATTGTTCGCCATGATCAGTTGATGACGTTAG
TTGAGTGGCTGGGGCAGAGATCTACTACTATCTTGCGTGAGATGTCCCAAGATCAGTTGGATGTTGGAGCATCGCAATCA
CTATACATTGATAATGAAGAGGCTGAAGATGGGCCTATTGCGCGGTTCATTCATAAAACTCTATCTGATGCATTAAATGC
AGGTGCTTCGGATATTCATTTTGAGTTTTATGAGCATATGGCACGTGTGCGGTTCCGTGTAGATGGGCAGTTGAGAGAAG
TAGTCCAGCCTCCTGTTGCAATACGTGGGCAGCTAGCATCACGCATTAAGGTAATGGCAAGGTTGGATATTTCTGAAAAA
CGTATTCCTCAAGATGGGCGCATCCAAATCGCTTTTTCTAAACATGGTAGACCCACAGATTTTCGTGTGAGTACTTTGCC
AACTTTGTTTGGAGAGAAAGTTGTGATGCGTATTCTTAATTCGGATGCAGCATCGCTAAATATTGATCAGCTCGGGTTTG
AAGATTTTCAAAAGGAATTATTACTGGAGGCAATCCATCGGCCATATGGGATGGTTTTAGTAACTGGTCCGACAGGTTCT
GGTAAAACTGTTTCTTTGTATACTTGTCTAAACATTTTAAATACCGAGGGAGTAAATATTTCCACAGCAGAAGATCCGGC
GGAAATTAATTTGCCTGGAATTAACCAAGTAAATGTTAATGATAAACAAGGATTAACTTTTGCAGCTGCACTAAAATCGT
TTCTGCGCCAAGATCCGGATATTATTATGGTTGGTGAGATTCGTGATTTGGAAACTGCAGATATTGCCATTAAAGCATCG
CAGACAGGGCATATGGTGTTTTCTACATTGCATACCAATAATGCTCCTGCAACATTGTCTCGTATGCTGAATATGGGTGT
CGCACCATTTAATATAGCCAGTTCCGTTAGTCTCATTATGGCACAAAGGTTATTGCGTAGATTGTGCCCAAATTGTAAGG
AACCAATGGAAAGACCTCCTGAGCATGCCTTGAGAAAGGCAGGTTTTTCAGATGAAGATTTAGCTAAAGATTGGACAATG
TATCGAGCGGTAGGTTGTGATACATGCAGAGGTAAAGGTTATAAGGGGCGTGCTGGTGTATATGAAGTAATGCCAATTAC
AGATGAAATGCAAAGAGTGATTATGGAAGGTGGAACAGAAGTAGATATTCAAAAAATGGCATATCAAGAGGGAATGGTAG
ATCTACGTCGAGCAGGTTTATTGAAGGTAATGAGCGGTATTACTTCTCTAGAAGAAGTATTAGCTCATACCAATGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

75.671

100

0.757
  pilB Acinetobacter baumannii D1279779

51.601

100

0.519
  pilB Acinetobacter baylyi ADP1

50.626

100

0.506
  pilB Legionella pneumophila strain ERS1305867

47.95

100

0.481
  pilB Vibrio cholerae strain A1552

46.516

100

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

47.249

94.275

0.445
  pilB Vibrio campbellii strain DS40M4

45.92

94.275

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.885

93.739

0.374
  pilF Thermus thermophilus HB27

38.258

94.454

0.361

Multiple sequence alignment