Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DV401_RS04780 Genome accession   NZ_CP031254
Coordinates   935904..937298 (+) Length   464 a.a.
NCBI ID   WP_114877323.1    Uniprot ID   -
Organism   Haemophilus influenzae strain M25588     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 930904..942298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV401_RS04755 rsmE 930923..931660 (-) 738 WP_014550478.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DV401_RS04760 lnt 931710..933239 (-) 1530 WP_080481790.1 apolipoprotein N-acyltransferase -
  DV401_RS04765 corC 933262..934161 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  DV401_RS04770 ampD 934792..935343 (-) 552 WP_071162800.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DV401_RS04775 pilA 935458..935907 (+) 450 WP_103708055.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DV401_RS04780 pilB 935904..937298 (+) 1395 WP_114877323.1 GspE/PulE family protein Machinery gene
  DV401_RS04785 pilC 937295..938512 (+) 1218 WP_114877325.1 type II secretion system F family protein Machinery gene
  DV401_RS04790 pilD 938509..939201 (+) 693 WP_114877327.1 prepilin peptidase Machinery gene
  DV401_RS04795 rho 939255..940517 (-) 1263 WP_005666690.1 transcription termination factor Rho -
  DV401_RS04800 metJ 940752..941069 (+) 318 WP_005634287.1 met regulon transcriptional regulator MetJ -
  DV401_RS04805 cueR 941083..941469 (-) 387 WP_114877329.1 Cu(I)-responsive transcriptional regulator -
  DV401_RS04810 - 941546..941752 (+) 207 WP_005654130.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52873.26 Da        Isoelectric Point: 5.2105

>NTDB_id=305105 DV401_RS04780 WP_114877323.1 935904..937298(+) (pilB) [Haemophilus influenzae strain M25588]
MTSYALLHTQRVTAQNGEIFTISPDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPNQMQVEEQVKFYQHQETYFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=305105 DV401_RS04780 WP_114877323.1 935904..937298(+) (pilB) [Haemophilus influenzae strain M25588]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATATTTACGATCTCGCCTGATTTATG
GGCGCGTAATCAACAACAGCAATCCTTGCTCTTACGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGAACCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCTATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCTAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGTTTTGCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACACGCACTTAGCCAACCCCAAGGATTAATTTTAGTAACTGGCCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAGGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGTGATGAAGAAAGTGCGATGATTGCACTACGTGC
CGCTCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTCGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

98.491

100

0.985
  pilB Haemophilus influenzae Rd KW20

96.544

99.784

0.963
  pilB Glaesserella parasuis strain SC1401

57.734

98.922

0.571
  pilB Vibrio cholerae strain A1552

40.038

100

0.455
  pilB Vibrio campbellii strain DS40M4

40.491

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.166

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.673

100

0.418
  pilB Acinetobacter baylyi ADP1

38.306

100

0.409
  pilB Acinetobacter baumannii D1279779

39.085

100

0.405
  pilF Neisseria gonorrhoeae MS11

36.975

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377
  pilF Thermus thermophilus HB27

36.008

100

0.377

Multiple sequence alignment