Detailed information    

insolico Bioinformatically predicted

Overview

Name   comL   Type   Machinery gene
Locus tag   EW646_RS16540 Genome accession   NZ_CP035770
Coordinates   3325821..3326558 (+) Length   245 a.a.
NCBI ID   WP_000197686.1    Uniprot ID   P0AC03
Organism   Escherichia coli O145 strain RM8988-C1     
Function   DNA binding (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3320821..3331558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW646_RS16525 (EW646_16985) clpC 3321275..3323848 (-) 2574 WP_001235102.1 ATP-dependent chaperone ClpB Regulator
  EW646_RS16530 (EW646_16990) yfiH 3323978..3324709 (-) 732 WP_097455658.1 purine nucleoside phosphorylase YfiH -
  EW646_RS16535 (EW646_16995) rluD 3324706..3325686 (-) 981 WP_000079110.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  EW646_RS16540 (EW646_17000) comL 3325821..3326558 (+) 738 WP_000197686.1 outer membrane protein assembly factor BamD Machinery gene
  EW646_RS16545 (EW646_17005) raiA 3326829..3327179 (+) 351 WP_000178457.1 ribosome-associated translation inhibitor RaiA -
  EW646_RS16550 (EW646_17010) pheL 3327283..3327330 (+) 48 WP_001386991.1 pheA operon leader peptide PheL -
  EW646_RS16555 (EW646_17015) pheA 3327429..3328589 (+) 1161 WP_000200122.1 bifunctional chorismate mutase/prephenate dehydratase -
  EW646_RS16560 (EW646_17020) tyrA 3328632..3329753 (-) 1122 WP_000225204.1 bifunctional chorismate mutase/prephenate dehydrogenase -
  EW646_RS16565 (EW646_17025) aroF 3329764..3330834 (-) 1071 WP_001168045.1 3-deoxy-7-phosphoheptulonate synthase AroF -
  EW646_RS16570 (EW646_17030) yfiL 3331044..3331409 (+) 366 WP_000976004.1 DUF2799 domain-containing protein -

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27829.40 Da        Isoelectric Point: 6.4874

>NTDB_id=304820 EW646_RS16540 WP_000197686.1 3325821..3326558(+) (comL) [Escherichia coli O145 strain RM8988-C1]
MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQDGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYY
KNADLPLAQAAIDRFIRLNPTHPNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLMENAYRQMQMNAQAEKVAKIIAA
NSSNT

Nucleotide


Download         Length: 738 bp        

>NTDB_id=304820 EW646_RS16540 WP_000197686.1 3325821..3326558(+) (comL) [Escherichia coli O145 strain RM8988-C1]
ATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGT
ACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGC
AACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTAT
AAAAACGCCGATTTGCCGTTAGCGCAGGCTGCCATCGATCGTTTTATTCGCCTTAACCCGACCCATCCGAATATCGATTA
TGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTTGACCGTAGCG
ATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACC
ACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTAGCCGAGTACTATACAGA
ACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATG
CGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCA
AACAGCAGCAATACATAA

Domains

Predicted by InterProScan.

Outer membrane lipoprotein (YfiO)

(28-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0AC03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comL Neisseria meningitidis MC58

38.525

99.592

0.384
  comL Neisseria gonorrhoeae MS11

37.705

99.592

0.376

Multiple sequence alignment