Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   DV129_RS09135 Genome accession   NZ_CP031247
Coordinates   1688707..1689723 (+) Length   338 a.a.
NCBI ID   WP_001844679.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain M23734     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1683707..1694723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV129_RS09115 nagA 1684854..1686005 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  DV129_RS09120 - 1686168..1687226 (+) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  DV129_RS09125 - 1687377..1687742 (+) 366 WP_000286415.1 DUF1033 family protein -
  DV129_RS09130 comGA/cglA/cilD 1687818..1688759 (+) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  DV129_RS09135 comGB/cglB 1688707..1689723 (+) 1017 WP_001844679.1 competence type IV pilus assembly protein ComGB Machinery gene
  DV129_RS09140 comGC/cglC 1689725..1690051 (+) 327 WP_000738633.1 comG operon protein ComGC Machinery gene
  DV129_RS09145 comGD/cglD 1690044..1690448 (+) 405 WP_000588020.1 competence type IV pilus minor pilin ComGD Machinery gene
  DV129_RS09150 comGE/cglE 1690411..1690713 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  DV129_RS09155 comGF/cglF 1690676..1691137 (+) 462 WP_000250531.1 competence type IV pilus minor pilin ComGF Machinery gene
  DV129_RS09160 comGG/cglG 1691115..1691528 (+) 414 WP_000265631.1 competence type IV pilus minor pilin ComGG Machinery gene
  DV129_RS09170 - 1691665..1692259 (+) 595 Protein_1712 methyltransferase domain-containing protein -
  DV129_RS09175 comYH 1692320..1693273 (+) 954 WP_000345116.1 class I SAM-dependent methyltransferase Machinery gene
  DV129_RS09180 - 1693324..1694514 (+) 1191 WP_000167766.1 acetate kinase -
  DV129_RS12550 - 1694515..1694646 (+) 132 WP_000768904.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38535.63 Da        Isoelectric Point: 9.6162

>NTDB_id=304790 DV129_RS09135 WP_001844679.1 1688707..1689723(+) (comGB/cglB) [Streptococcus pneumoniae strain M23734]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVIQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMAGLVSVLALLALTFYKRSSKMNVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=304790 DV129_RS09135 WP_001844679.1 1688707..1689723(+) (comGB/cglB) [Streptococcus pneumoniae strain M23734]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGATCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGTTTTTTCTTCTCTTAATCATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGCAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAATGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae D39

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae R6

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae TIGR4

98.225

100

0.982
  comGB/cglB Streptococcus mitis SK321

94.379

100

0.944
  comGB/cglB Streptococcus mitis NCTC 12261

93.787

100

0.938
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.044

93.787

0.544
  comYB Streptococcus mutans UA159

58.044

93.787

0.544
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment