Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DV365_RS01250 Genome accession   NZ_CP031237
Coordinates   238146..239540 (+) Length   464 a.a.
NCBI ID   WP_114890120.1    Uniprot ID   -
Organism   Haemophilus influenzae strain M12125     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 233146..244540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV365_RS01225 rsmE 233158..233895 (-) 738 WP_012054951.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DV365_RS01230 lnt 233947..235476 (-) 1530 WP_114890118.1 apolipoprotein N-acyltransferase -
  DV365_RS01235 corC 235499..236398 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  DV365_RS01240 ampD 237022..237582 (-) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DV365_RS01245 pilA 237697..238149 (+) 453 WP_114890119.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DV365_RS01250 pilB 238146..239540 (+) 1395 WP_114890120.1 GspE/PulE family protein Machinery gene
  DV365_RS01255 pilC 239537..240754 (+) 1218 WP_114890121.1 type II secretion system F family protein Machinery gene
  DV365_RS01260 pilD 240751..241446 (+) 696 WP_114890122.1 A24 family peptidase Machinery gene
  DV365_RS01265 rho 241502..242764 (-) 1263 WP_114890123.1 transcription termination factor Rho -
  DV365_RS01270 metJ 243000..243317 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  DV365_RS01275 - 243331..243708 (-) 378 WP_075913212.1 Cu(I)-responsive transcriptional regulator -
  DV365_RS01280 - 243785..243991 (+) 207 WP_015701556.1 heavy-metal-associated domain-containing protein -
  DV365_RS01285 - 244065..244271 (+) 207 WP_015701556.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52969.40 Da        Isoelectric Point: 5.6399

>NTDB_id=304358 DV365_RS01250 WP_114890120.1 238146..239540(+) (pilB) [Haemophilus influenzae strain M12125]
MTSYALLHTQRVTAQNGEVFTISPDLWARNQQQHSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQHLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTYWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=304358 DV365_RS01250 WP_114890120.1 238146..239540(+) (pilB) [Haemophilus influenzae strain M12125]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGGTATTTACGATCTCGCCTGATTTATG
GGCGCGTAATCAACAACAGCACTCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGCCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACACTTAATAAGCAAAATATTCGCTAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCTATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTAACTGGCCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAGGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGTGATGAAGAAAGTGCGATGATTGCACTACGTGC
CGCTCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTTGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

98.06

100

0.981
  pilB Haemophilus influenzae Rd KW20

96.328

99.784

0.961
  pilB Glaesserella parasuis strain SC1401

57.675

98.276

0.567
  pilB Vibrio cholerae strain A1552

39.771

100

0.448
  pilB Vibrio campbellii strain DS40M4

40.574

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.289

100

0.42
  pilB Legionella pneumophila strain ERS1305867

39.673

100

0.418
  pilB Acinetobacter baylyi ADP1

38.306

100

0.409
  pilB Acinetobacter baumannii D1279779

38.831

100

0.401
  pilF Thermus thermophilus HB27

36.701

100

0.384
  pilF Neisseria gonorrhoeae MS11

36.611

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377

Multiple sequence alignment