Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DV365_RS01245 Genome accession   NZ_CP031237
Coordinates   237697..238149 (+) Length   150 a.a.
NCBI ID   WP_114890119.1    Uniprot ID   -
Organism   Haemophilus influenzae strain M12125     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 232697..243149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV365_RS01225 rsmE 233158..233895 (-) 738 WP_012054951.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DV365_RS01230 lnt 233947..235476 (-) 1530 WP_114890118.1 apolipoprotein N-acyltransferase -
  DV365_RS01235 corC 235499..236398 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  DV365_RS01240 ampD 237022..237582 (-) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DV365_RS01245 pilA 237697..238149 (+) 453 WP_114890119.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DV365_RS01250 pilB 238146..239540 (+) 1395 WP_114890120.1 GspE/PulE family protein Machinery gene
  DV365_RS01255 pilC 239537..240754 (+) 1218 WP_114890121.1 type II secretion system F family protein Machinery gene
  DV365_RS01260 pilD 240751..241446 (+) 696 WP_114890122.1 A24 family peptidase Machinery gene
  DV365_RS01265 rho 241502..242764 (-) 1263 WP_114890123.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15392.77 Da        Isoelectric Point: 8.7960

>NTDB_id=304357 DV365_RS01245 WP_114890119.1 237697..238149(+) (pilA) [Haemophilus influenzae strain M12125]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTGKPSSCSGGSNGIA
ADIKTAKGYVASVITQSGGITVKGNGTLANMEYILQASGNAAAAGVTWTTTCKGTDASLFPANFCGSVAQ

Nucleotide


Download         Length: 453 bp        

>NTDB_id=304357 DV365_RS01245 WP_114890119.1 237697..238149(+) (pilA) [Haemophilus influenzae strain M12125]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCTTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCAC
CTTATAAGGCTGATGTGGAATTATGCGTTTATAGCACAGGTAAACCTTCCAGTTGCTCGGGAGGAAGCAATGGTATTGCA
GCGGATATAAAGACAGCAAAAGGCTATGTAGCCTCAGTAATCACTCAATCAGGTGGCATTACAGTAAAAGGGAATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCCAGTGGTAATGCTGCAGCAGCAGGTGTAACTTGGACAACAACTTGCAAAG
GAACGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCGCACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

92.667

100

0.927
  pilA Haemophilus influenzae 86-028NP

88

100

0.88
  pilA Glaesserella parasuis strain SC1401

55.556

100

0.567
  pilA2 Legionella pneumophila strain ERS1305867

38.255

99.333

0.38
  pilA2 Legionella pneumophila str. Paris

42.424

88

0.373

Multiple sequence alignment