Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MG068_RS16405 Genome accession   NZ_CP031167
Coordinates   3530242..3531501 (+) Length   419 a.a.
NCBI ID   WP_132810681.1    Uniprot ID   -
Organism   Stenotrophomonas sp. ASS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3525242..3536501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MG068_RS16390 (MG068_16345) pilR 3525820..3527193 (+) 1374 WP_032128822.1 sigma-54 dependent transcriptional regulator Regulator
  MG068_RS16395 (MG068_16350) pilB 3527420..3529147 (-) 1728 WP_132810679.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MG068_RS16400 (MG068_16355) - 3529438..3529881 (-) 444 WP_132810680.1 pilin -
  MG068_RS16405 (MG068_16360) pilC 3530242..3531501 (+) 1260 WP_132810681.1 type II secretion system F family protein Machinery gene
  MG068_RS16410 (MG068_16365) - 3531510..3532373 (+) 864 WP_032128827.1 A24 family peptidase -
  MG068_RS16415 (MG068_16370) coaE 3532385..3532996 (+) 612 WP_132810682.1 dephospho-CoA kinase -
  MG068_RS16420 (MG068_16375) - 3533159..3534526 (-) 1368 WP_032128829.1 HAMP domain-containing sensor histidine kinase -
  MG068_RS16425 (MG068_16380) - 3534489..3535166 (-) 678 WP_005410804.1 response regulator transcription factor -
  MG068_RS16430 (MG068_16385) - 3535175..3535648 (-) 474 WP_049425106.1 hypothetical protein -
  MG068_RS16435 (MG068_16390) - 3535738..3536214 (-) 477 WP_132810683.1 hypothetical protein -

Sequence

Protein


Download         Length: 419 a.a.        Molecular weight: 45757.16 Da        Isoelectric Point: 10.2043

>NTDB_id=303839 MG068_RS16405 WP_132810681.1 3530242..3531501(+) (pilC) [Stenotrophomonas sp. ASS1]
MSVSRSAIKKEPVARSTMELQPFVWEGTDKRGIKMKGEQLAKNANLLRAELRKQGINPGQVKPKPKPLFGAAGKPVGAKD
IAFFSRQMATMMKSGVPIVSALEIIGSGHKNPRMKKMVDGIRADIEGGSSLNEAISRHPVQFDELYRNLVRAGESAGVLE
TVLDTIASYKENIEALKGKIKKALFYPAMVLVVAFLVSTVLLVWVVPQFEEVFKSFGADLPAFTQMVVNLSRFMVSWWWL
IAIVVIGAVVAIAMTYRRSEKMQHTVDRLVLKVPVIGQIMHNSSIARFARTTAVTFKAGVPLVEALGIVAGATGNKVYGE
AVLRMRDDVSVGYPVNMAMKQLNLFPHMVIQMTGIGEEAGALDAMLFKVAEYYEQEVNNAVDALSSLLEPMIMVFIGTIV
GGLVIAMYLPIFKLGAVVG

Nucleotide


Download         Length: 1260 bp        

>NTDB_id=303839 MG068_RS16405 WP_132810681.1 3530242..3531501(+) (pilC) [Stenotrophomonas sp. ASS1]
ATGTCTGTCAGTCGCAGTGCGATCAAGAAAGAGCCCGTGGCGCGTAGCACCATGGAGCTGCAGCCGTTTGTCTGGGAGGG
GACCGACAAGCGGGGTATCAAGATGAAGGGCGAGCAGTTGGCCAAGAACGCCAATCTGCTGCGCGCCGAATTACGCAAGC
AGGGAATCAATCCCGGCCAAGTGAAGCCGAAGCCGAAGCCACTGTTCGGCGCGGCCGGCAAGCCCGTGGGCGCTAAGGAC
ATTGCGTTCTTCAGCCGGCAAATGGCCACGATGATGAAATCCGGCGTGCCGATCGTGTCGGCGCTGGAGATCATCGGCAG
CGGGCACAAGAACCCGCGCATGAAGAAGATGGTGGACGGGATCCGCGCCGACATCGAGGGCGGCTCTTCTCTGAACGAGG
CGATCAGCCGTCACCCTGTCCAGTTCGACGAGCTCTACCGCAACCTGGTACGCGCCGGTGAAAGCGCCGGTGTACTGGAA
ACGGTGCTGGATACGATCGCCTCCTACAAGGAGAACATCGAGGCGCTGAAGGGCAAGATCAAGAAGGCCCTGTTCTACCC
GGCCATGGTGCTGGTGGTGGCCTTCCTGGTCAGCACTGTCCTGCTGGTCTGGGTCGTGCCGCAATTCGAGGAGGTCTTCA
AGAGTTTCGGTGCCGACCTGCCGGCCTTCACCCAGATGGTGGTCAACCTGTCCCGCTTCATGGTGTCGTGGTGGTGGCTG
ATAGCCATCGTCGTGATCGGAGCGGTGGTGGCCATCGCCATGACCTACCGGCGTTCGGAGAAGATGCAGCACACGGTCGA
CCGGCTGGTCCTGAAGGTGCCGGTGATCGGCCAGATCATGCATAACAGCTCGATTGCCCGTTTCGCCCGCACCACGGCTG
TGACCTTCAAGGCCGGCGTGCCGCTGGTGGAGGCGCTGGGCATCGTGGCTGGCGCCACCGGCAACAAGGTCTATGGCGAA
GCGGTCCTGCGCATGCGCGACGACGTCTCGGTGGGCTATCCGGTCAACATGGCCATGAAGCAGCTGAACCTGTTCCCGCA
CATGGTCATCCAGATGACCGGCATCGGCGAAGAGGCGGGCGCCCTGGACGCCATGCTGTTCAAGGTGGCTGAATACTACG
AGCAGGAAGTGAACAATGCCGTGGATGCCTTGAGCAGCCTGCTGGAACCGATGATCATGGTGTTCATTGGTACCATTGTC
GGTGGCTTGGTCATCGCGATGTACCTGCCCATCTTCAAACTCGGCGCCGTCGTCGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(84-207)

Type II secretion system (T2SS), protein F (T2SSF)

(288-410)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.283

94.511

0.504
  pilC Legionella pneumophila strain ERS1305867

52.381

95.227

0.499
  pilC Acinetobacter baylyi ADP1

50.739

96.897

0.492
  pilC Acinetobacter baumannii D1279779

50.882

94.749

0.482
  pilG Neisseria gonorrhoeae MS11

43.577

94.749

0.413
  pilG Neisseria meningitidis 44/76-A

43.325

94.749

0.411
  pilC Vibrio cholerae strain A1552

41.133

96.897

0.399
  pilC Vibrio campbellii strain DS40M4

40.648

95.704

0.389

Multiple sequence alignment