Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MG068_RS16395 Genome accession   NZ_CP031167
Coordinates   3527420..3529147 (-) Length   575 a.a.
NCBI ID   WP_132810679.1    Uniprot ID   -
Organism   Stenotrophomonas sp. ASS1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3522420..3534147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MG068_RS16380 (MG068_16335) sucC 3522571..3523740 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  MG068_RS16385 (MG068_16340) - 3524146..3525759 (+) 1614 WP_049401617.1 ATP-binding protein -
  MG068_RS16390 (MG068_16345) pilR 3525820..3527193 (+) 1374 WP_032128822.1 sigma-54 dependent transcriptional regulator Regulator
  MG068_RS16395 (MG068_16350) pilB 3527420..3529147 (-) 1728 WP_132810679.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MG068_RS16400 (MG068_16355) - 3529438..3529881 (-) 444 WP_132810680.1 pilin -
  MG068_RS16405 (MG068_16360) pilC 3530242..3531501 (+) 1260 WP_132810681.1 type II secretion system F family protein Machinery gene
  MG068_RS16410 (MG068_16365) - 3531510..3532373 (+) 864 WP_032128827.1 A24 family peptidase -
  MG068_RS16415 (MG068_16370) coaE 3532385..3532996 (+) 612 WP_132810682.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62252.19 Da        Isoelectric Point: 6.1552

>NTDB_id=303838 MG068_RS16395 WP_132810679.1 3527420..3529147(-) (pilB) [Stenotrophomonas sp. ASS1]
MSTLSTPNLVGITGLARRLVQDGALDEASARDAMAKATAARQPLPTWFAQNKLVSASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHALDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTIGDALGGD
DEGMANLDVGLGDEDNAGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFH
DAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPANLPEHA
LLAEGFTQAQLDAGIQLYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKAA
AGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=303838 MG068_RS16395 WP_132810679.1 3527420..3529147(-) (pilB) [Stenotrophomonas sp. ASS1]
ATGAGCACTCTTTCCACCCCCAATCTCGTAGGCATCACCGGCCTGGCCCGCCGCCTGGTCCAGGACGGCGCGCTGGACGA
GGCCTCCGCCCGCGACGCCATGGCCAAGGCCACGGCCGCCCGCCAGCCGCTGCCTACGTGGTTCGCGCAGAACAAGCTGG
TCAGTGCCTCGCAGCTCGCCGCCGCCAACGCGGTCGAGTTCGGCATGCCCTTGTTCGACGTGTCTACGTTCGACGCCAGC
CAGAACGCCATGAGCCTGGTCAGCGAGGAACTGCTGCGCAAACACAACGTCCTGCCGCTGTTCAAGCGCGGTGGCAAGCT
GTTCGTGGGTACCAGCAATCCAACCCATGCACTGGACGAGATCAAGTTCCACACCAATCTGGTGGTGGAGCCGATCCTGG
TGGATGAAGACCAGATCCGCCGCACGCTGGAGCAGTGGCACGCCAGCCACGACACCATCGGCGATGCGCTGGGCGGCGAC
GACGAGGGCATGGCCAACCTCGACGTTGGACTGGGCGACGAAGACAACGCGGGGGGCGACTCCGGCATCGACGCCAAGGG
CGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCCATCCGCAAGGGCGCATCGGACATCCACTTCG
AGCCCTACGAAGACGATTACCGCGTGCGCTTGCGTATCGACGGTCTGCTGAAGATGGTGGCGCGCGCGCCGGTGAAGTTG
AACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCCGAGAAGCGCGTGCCGCAGGACGGCCGCAT
CAAGCTGAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGTACGCTGCCGACCCTGTTCGGTGAAAAGGTGGTGC
TGCGTATTCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAGCTGTTCCAC
GACGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCGGGCAAAACCGTGTCGCTGTATACCGC
GCTGGGCATCCTCAACGATGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCTTGCCCGGCGTCAACC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCTGCAGCGCTGCGCTCGTTCCTGCGACAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTCCAC
GCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCTCGG
TGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGGTTGTGCGGCAACTGCAAGCGACCGGCGAACCTGCCGGAGCACGCG
CTGCTGGCCGAGGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCCAGTTGTACGAGGCGGTGGGCTGCGATGAGTGCAC
CGAGGGCTACAAGGGCCGAACCGGTATCTACCAGGTGATGCCGATGACCGATGAGATCGCCACGATCGTGCTGGCCGGCG
GCAACGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGGCAGTCGGCGTTGAAGAAGGCGGCA
GCGGGTGTGACCAGCCTGGCCGAGATCAATCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.401

100

0.567
  pilB Acinetobacter baylyi ADP1

56.055

100

0.563
  pilB Legionella pneumophila strain ERS1305867

51.313

99.304

0.51
  pilB Vibrio cholerae strain A1552

48.601

99.478

0.483
  pilF Neisseria gonorrhoeae MS11

48.768

98.783

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

45.884

99.304

0.456
  pilB Vibrio campbellii strain DS40M4

44.991

98.957

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

36.007

100

0.383
  pilF Thermus thermophilus HB27

37.589

98.087

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.462

94.957

0.365

Multiple sequence alignment