Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EUJ32_RS19390 Genome accession   NZ_CP035486
Coordinates   3947532..3949028 (-) Length   498 a.a.
NCBI ID   WP_001040647.1    Uniprot ID   A0A0H2VBP4
Organism   Escherichia coli strain NissleGFP_pZE21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3942532..3954028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUJ32_RS19365 (EUJ32_19365) panM 3942766..3943149 (+) 384 WP_000778808.1 aspartate 1-decarboxylase autocleavage activator PanM -
  EUJ32_RS19370 (EUJ32_19370) livJ 3943337..3944440 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  EUJ32_RS19375 (EUJ32_19375) rpoH 3944711..3945565 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  EUJ32_RS19380 (EUJ32_19380) ftsX 3945810..3946868 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  EUJ32_RS19385 (EUJ32_19385) ftsE 3946861..3947529 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  EUJ32_RS19390 (EUJ32_19390) pilA 3947532..3949028 (-) 1497 WP_001040647.1 signal recognition particle-docking protein FtsY Machinery gene
  EUJ32_RS19395 (EUJ32_19395) rsmD 3949178..3949774 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  EUJ32_RS19400 (EUJ32_19400) yhhL 3949764..3950033 (+) 270 WP_001295207.1 DUF1145 family protein -
  EUJ32_RS19405 (EUJ32_19405) yhhM 3950036..3950395 (-) 360 WP_000042886.1 DUF2500 domain-containing protein -
  EUJ32_RS19410 (EUJ32_19410) yhhN 3950536..3951162 (+) 627 WP_000964718.1 lysoplasmalogenase -
  EUJ32_RS19415 (EUJ32_19415) zntA 3951236..3953434 (+) 2199 WP_000106605.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  EUJ32_RS19420 (EUJ32_19420) tusA 3953695..3953940 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54627.45 Da        Isoelectric Point: 4.1988

>NTDB_id=303838 EUJ32_RS19390 WP_001040647.1 3947532..3949028(-) (pilA) [Escherichia coli strain NissleGFP_pZE21]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAKAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLSEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEVAEEAVMVVSPAEEDQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=303838 EUJ32_RS19390 WP_001040647.1 3947532..3949028(-) (pilA) [Escherichia coli strain NissleGFP_pZE21]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCAAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGTGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGATCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACAGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCTGATTCCGCTTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VBP4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378

Multiple sequence alignment