Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DUN60_RS11585 Genome accession   NZ_CP031055
Coordinates   2657428..2657901 (+) Length   157 a.a.
NCBI ID   WP_054547487.1    Uniprot ID   -
Organism   Vibrio splendidus strain Vibrio sp     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2652428..2662901
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DUN60_RS11565 pdhR 2654356..2655117 (-) 762 WP_017629813.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  DUN60_RS11575 ampD 2655598..2656146 (-) 549 WP_114633998.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DUN60_RS11580 nadC 2656282..2657172 (+) 891 WP_114633999.1 carboxylating nicotinate-nucleotide diphosphorylase -
  DUN60_RS11585 pilA 2657428..2657901 (+) 474 WP_054547487.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DUN60_RS11590 pilB 2657901..2659586 (+) 1686 WP_054547486.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DUN60_RS11595 pilC 2659601..2660830 (+) 1230 WP_054547485.1 type II secretion system F family protein Machinery gene
  DUN60_RS11600 pilD 2660898..2661767 (+) 870 WP_114634000.1 A24 family peptidase Machinery gene
  DUN60_RS11605 coaE 2661770..2662384 (+) 615 WP_054547484.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16536.04 Da        Isoelectric Point: 8.7912

>NTDB_id=302962 DUN60_RS11585 WP_054547487.1 2657428..2657901(+) (pilA) [Vibrio splendidus strain Vibrio sp]
MNNKNKRTNQKGFTLIELMIVVAIIGILSAFAVPAYQDYTKKATLSEFPKAAAAVKMAVELCAHESASDAATFKTSCISN
NNGVPNVLTLNNMEIKAIGGSASGAVDVRIKASATKGPIKTGETYVMTATYAAVGITWATKCYTDAALANTQDTYCP

Nucleotide


Download         Length: 474 bp        

>NTDB_id=302962 DUN60_RS11585 WP_054547487.1 2657428..2657901(+) (pilA) [Vibrio splendidus strain Vibrio sp]
ATGAATAACAAAAATAAAAGAACAAATCAGAAAGGTTTTACGTTAATTGAATTGATGATTGTGGTGGCGATTATTGGGAT
CTTATCGGCATTTGCTGTGCCTGCTTATCAAGATTACACAAAAAAAGCGACTCTATCTGAATTTCCAAAAGCCGCCGCTG
CTGTAAAAATGGCTGTTGAATTGTGTGCTCATGAAAGTGCTTCCGATGCTGCGACATTTAAAACTAGCTGTATCTCTAAC
AATAATGGCGTCCCGAATGTGCTAACACTTAACAACATGGAAATTAAAGCTATCGGTGGGAGCGCTTCTGGTGCTGTCGA
TGTTCGCATAAAAGCATCGGCAACCAAAGGACCTATAAAAACTGGCGAGACCTATGTTATGACAGCAACTTATGCTGCTG
TAGGTATTACTTGGGCAACTAAATGTTACACCGATGCCGCACTTGCTAATACACAAGATACTTATTGCCCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

58.824

97.452

0.573
  pilA2 Legionella pneumophila str. Paris

40.94

94.904

0.389
  pilA Haemophilus influenzae 86-028NP

41.379

92.357

0.382
  pilA2 Legionella pneumophila strain ERS1305867

40.268

94.904

0.382
  comP Acinetobacter baylyi ADP1

41.549

90.446

0.376
  pilA Haemophilus influenzae Rd KW20

40.278

91.72

0.369

Multiple sequence alignment