Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DUN60_RS11590 Genome accession   NZ_CP031055
Coordinates   2657901..2659586 (+) Length   561 a.a.
NCBI ID   WP_054547486.1    Uniprot ID   A0A837NTR5
Organism   Vibrio splendidus strain Vibrio sp     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2652901..2664586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DUN60_RS11565 pdhR 2654356..2655117 (-) 762 WP_017629813.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  DUN60_RS11575 ampD 2655598..2656146 (-) 549 WP_114633998.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DUN60_RS11580 nadC 2656282..2657172 (+) 891 WP_114633999.1 carboxylating nicotinate-nucleotide diphosphorylase -
  DUN60_RS11585 pilA 2657428..2657901 (+) 474 WP_054547487.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DUN60_RS11590 pilB 2657901..2659586 (+) 1686 WP_054547486.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DUN60_RS11595 pilC 2659601..2660830 (+) 1230 WP_054547485.1 type II secretion system F family protein Machinery gene
  DUN60_RS11600 pilD 2660898..2661767 (+) 870 WP_114634000.1 A24 family peptidase Machinery gene
  DUN60_RS11605 coaE 2661770..2662384 (+) 615 WP_054547484.1 dephospho-CoA kinase -
  DUN60_RS11610 zapD 2662411..2663151 (+) 741 WP_017087400.1 cell division protein ZapD -
  DUN60_RS11615 yacG 2663233..2663430 (+) 198 WP_004735515.1 DNA gyrase inhibitor YacG -
  DUN60_RS11620 rplS 2663757..2664110 (-) 354 WP_004735514.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62187.83 Da        Isoelectric Point: 4.9243

>NTDB_id=302963 DUN60_RS11590 WP_054547486.1 2657901..2659586(+) (pilB) [Vibrio splendidus strain Vibrio sp]
MLTNLPTVLRQADLLSLTQEQAVAEHVQVSGVSTPEALLVLDFFTGDSLANNIKTIFGLPLVQLASTDYETLCEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVEISDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLSHMGIEPFNLASSLSLIIAQRLARRLCNHCKAADDSPDIFLRHSIPNNQT
IYKANPQGCNECNQGYSGRVGIYEVMPFSDQLKNSLIDKPNALAIENLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=302963 DUN60_RS11590 WP_054547486.1 2657901..2659586(+) (pilB) [Vibrio splendidus strain Vibrio sp]
ATGCTAACCAACCTTCCAACCGTTCTACGTCAGGCTGATCTACTTAGCCTAACTCAAGAACAGGCCGTTGCGGAACATGT
GCAAGTTTCAGGTGTTTCTACACCTGAAGCTTTACTCGTTCTTGATTTCTTCACCGGCGATTCCTTAGCAAACAATATCA
AAACCATCTTCGGCTTACCGTTAGTGCAACTAGCCAGTACTGACTACGAAACCTTGTGTGAGCAATTGGGGCTTCGTGAG
CTGATCACCAAATATCGAGCAATCCCGATTTCAGTTTCTAGTTCTACTCTTACGCTCGCCTCGGCTGATCCGACCGACTT
ACAAGCTGAAGACGACTTTCGATTCGCGACAGGATTGCAGATTGAATTGGTTGTCGCTAATTACTCTGAATTAGAAGGCG
CAATACGAAAGCTGTATGGCCGTTCTATTTCCGGGCAAGATTCCAAGCGCAAAGAGATAACCCAAGATGAGCTAGCCAAC
CTTGTTGAGATATCTGACGACGAGATAACGTCCATTGAAGACCTCAGCCAAGACGACTCCCCGGTTAGCCGCTTTATCAA
CCAGATACTAGTCGATGCGGTACGTAAAGGCGCTTCTGATATCCACTTCGAACCTTACGAAGAGCACTATCGTGTACGAC
TGCGCTGTGATGGTATTCTTGTTGAGATACAGCAGCCCGCTTCTCACTTAAGTCGTCGTCTATCGGCGCGCTTGAAGATC
CTCGCCAAACTTGATATCGCTGAACGGCGCTTGCCTCAAGATGGTCGTATTAAATTACGTTTAAATGACGAACTGGCGAT
TGATATGCGGGTATCGACGCTGCCAACATTATGGGGAGAAAAGATCGTTCTGCGTCTCTTAGACAGCAGCGCTGCCAATC
TCGATATCGATAAGCTCGGTTACAGCGAGGATCAAAAAGCACTCTACCTTAATGCATTGAAACGCCCGCAAGGGATGATC
TTAATGACCGGGCCAACCGGCAGTGGCAAAACCGTCTCTCTCTATACTGGCCTTCGAGTGCTCAACACCACTGAACGCAA
CATCTCAACAGCCGAAGATCCTGTGGAGATTAACCTGTGTGGCATCAATCAGGTACAAGTGACGCCTAAGATCGGCTTTG
GATTTGCTGAGGCGCTGCGATCATTTTTACGACAAGATCCTGATGTGGTGATGGTTGGAGAGATCCGCGACTTGGAAACC
GCAGAGATCGCGATCAAAGCCTCACAAACAGGCCACTTAGTGCTTTCGACACTGCACACCAATTCTGCAGCAGAAACCGT
GACTCGCCTCTCTCATATGGGGATAGAACCTTTTAACCTCGCTTCATCATTAAGCCTGATTATCGCCCAACGACTGGCAA
GACGATTGTGCAATCACTGTAAAGCCGCTGACGACTCTCCGGACATATTTCTGCGTCACTCTATCCCTAACAATCAAACG
ATCTACAAAGCCAATCCACAAGGATGCAATGAGTGTAATCAGGGATACTCGGGGCGAGTCGGTATCTATGAAGTCATGCC
TTTTAGCGACCAATTGAAAAATAGCCTGATAGACAAGCCGAACGCGTTAGCCATTGAAAACTTGGCACGCCGAGAAGGCA
TGAGAACTTTACAAGAATCAGGGCTAGATAAACTGCTTGAAGGCACCACCAGCTATCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A837NTR5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.179

100

0.734
  pilB Vibrio campbellii strain DS40M4

70.819

100

0.709
  pilB Vibrio parahaemolyticus RIMD 2210633

70.463

100

0.706
  pilB Acinetobacter baumannii D1279779

50.867

92.513

0.471
  pilB Legionella pneumophila strain ERS1305867

50.588

90.909

0.46
  pilB Acinetobacter baylyi ADP1

49.133

92.513

0.455
  pilF Neisseria gonorrhoeae MS11

43.604

98.93

0.431

Multiple sequence alignment