Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   DT070_RS10555 Genome accession   NZ_CP031013
Coordinates   2225666..2226883 (+) Length   405 a.a.
NCBI ID   WP_122955358.1    Uniprot ID   A0A5Q3HKT5
Organism   Polaromonas sp. SP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2220666..2231883
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DT070_RS10530 (DT070_10525) rpmA 2221847..2222104 (-) 258 WP_122955354.1 50S ribosomal protein L27 -
  DT070_RS10535 (DT070_10530) rplU 2222132..2222443 (-) 312 WP_122955355.1 50S ribosomal protein L21 -
  DT070_RS10540 (DT070_10535) - 2222640..2223623 (+) 984 WP_122955356.1 polyprenyl synthetase family protein -
  DT070_RS10550 (DT070_10545) pilB 2223848..2225581 (+) 1734 WP_122955357.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DT070_RS10555 (DT070_10550) pilC 2225666..2226883 (+) 1218 WP_122955358.1 type II secretion system F family protein Machinery gene
  DT070_RS10560 (DT070_10555) - 2226883..2227743 (+) 861 WP_122955359.1 A24 family peptidase -
  DT070_RS10565 (DT070_10560) coaE 2227748..2228359 (+) 612 WP_122955360.1 dephospho-CoA kinase -
  DT070_RS10570 (DT070_10565) zapD 2228403..2229158 (+) 756 WP_122957352.1 cell division protein ZapD -
  DT070_RS10575 (DT070_10570) - 2229205..2229426 (+) 222 WP_122955361.1 DNA gyrase inhibitor YacG -
  DT070_RS10580 (DT070_10575) - 2229540..2229827 (+) 288 WP_122955362.1 hypothetical protein -
  DT070_RS10585 (DT070_10580) grxD 2229862..2230191 (-) 330 WP_122955363.1 Grx4 family monothiol glutaredoxin -
  DT070_RS10590 (DT070_10585) prmC 2230358..2231230 (-) 873 WP_122955364.1 peptide chain release factor N(5)-glutamine methyltransferase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44142.08 Da        Isoelectric Point: 9.6171

>NTDB_id=302868 DT070_RS10555 WP_122955358.1 2225666..2226883(+) (pilC) [Polaromonas sp. SP1]
MATAVSKGVKEFVFEWEGKDRGGKQVRGEIRAVGENQVKSSLRRQGVLPTKIKKRKMRAGKSIKPKDIAIFTRQLATMMK
AGVPLLQAFDIVGRGNPNVSVTKLLNDVRTDVETGTSLSAAFRKYPMYFNALYCNLVEAGEAAGILESLLDRLAVYMEKT
EAIKSKIKSALMYPISVVVVAFVVVAVIMIFVIPAFKQVFSSFGADLPAPTLIVIAISEFFVAYWWLIFGGIGGSLYFFM
QAWKRNEKMQKFMDRLLLKVPVFGALIEKSCIARWTRTLSTMFAAGVPLVEALDSVGGASGNSLYADATEKIQQEVSTGT
SLTAAMTNANLFPTMVLQMCAIGEESGSVDHMLGKAADFYEAEVDEMVAGLSSLMEPIIIVFLGGLIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=302868 DT070_RS10555 WP_122955358.1 2225666..2226883(+) (pilC) [Polaromonas sp. SP1]
ATGGCAACTGCAGTATCCAAGGGCGTTAAGGAATTTGTTTTCGAATGGGAGGGCAAGGACCGCGGCGGCAAGCAGGTCCG
CGGTGAAATCCGTGCCGTCGGTGAAAACCAGGTCAAGTCCTCATTGCGTCGTCAGGGCGTATTACCCACCAAGATCAAGA
AGCGCAAGATGCGCGCCGGCAAGTCCATCAAGCCCAAGGACATCGCCATTTTTACGCGCCAATTGGCCACCATGATGAAG
GCGGGCGTTCCGCTTCTTCAGGCGTTTGACATCGTCGGCCGCGGTAATCCGAATGTCAGCGTCACCAAGCTGCTTAACGA
TGTGCGTACCGACGTTGAAACGGGTACGTCTCTCAGCGCGGCCTTTCGCAAGTACCCGATGTACTTCAATGCGCTTTATT
GCAACCTGGTCGAAGCGGGCGAAGCGGCCGGTATTCTGGAGTCTTTGCTGGACCGCCTTGCGGTCTACATGGAAAAAACC
GAGGCGATCAAGTCCAAGATCAAGTCGGCCCTGATGTACCCCATTTCAGTGGTGGTTGTTGCCTTTGTGGTGGTGGCGGT
GATCATGATTTTTGTGATCCCTGCGTTCAAGCAGGTGTTCTCATCTTTTGGGGCGGACCTTCCAGCCCCGACACTCATTG
TGATTGCGATTAGCGAATTTTTTGTTGCTTATTGGTGGCTGATTTTCGGCGGCATCGGCGGCAGCCTGTATTTCTTCATG
CAGGCCTGGAAACGCAATGAAAAGATGCAGAAATTCATGGACAGGCTGCTTTTGAAGGTGCCGGTCTTTGGCGCACTGAT
TGAGAAGTCCTGTATTGCCCGTTGGACTCGCACACTGTCCACCATGTTCGCGGCGGGTGTTCCGCTGGTGGAGGCACTTG
ACTCCGTCGGCGGCGCTTCCGGCAATTCCCTGTATGCCGATGCCACCGAGAAAATCCAGCAGGAAGTCTCTACCGGCACC
AGTTTGACGGCTGCGATGACCAACGCCAATCTGTTCCCCACCATGGTGCTGCAAATGTGCGCCATCGGAGAGGAATCCGG
CTCCGTTGACCACATGCTCGGTAAAGCAGCGGATTTCTATGAAGCGGAAGTCGATGAAATGGTGGCCGGCCTTTCAAGTT
TGATGGAGCCCATCATCATTGTGTTTCTGGGCGGGCTTATCGGGGGCATCGTGGTTTCCATGTACCTGCCCATCTTCAAG
CTGGGCCAAGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q3HKT5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.478

99.259

0.541
  pilC Legionella pneumophila strain ERS1305867

52.01

98.272

0.511
  pilG Neisseria gonorrhoeae MS11

51.25

98.765

0.506
  pilG Neisseria meningitidis 44/76-A

51

98.765

0.504
  pilC Acinetobacter baylyi ADP1

49.497

98.272

0.486
  pilC Acinetobacter baumannii D1279779

47.666

100

0.479
  pilC Vibrio cholerae strain A1552

40.452

98.272

0.398
  pilC Vibrio campbellii strain DS40M4

39.401

99.012

0.39
  pilC Thermus thermophilus HB27

38.861

99.753

0.388

Multiple sequence alignment