Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DT070_RS10550 Genome accession   NZ_CP031013
Coordinates   2223848..2225581 (+) Length   577 a.a.
NCBI ID   WP_122955357.1    Uniprot ID   A0A5Q3HLQ0
Organism   Polaromonas sp. SP1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2218848..2230581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DT070_RS10520 (DT070_10515) proB 2219396..2220532 (-) 1137 WP_122955352.1 glutamate 5-kinase -
  DT070_RS10525 (DT070_10520) cgtA 2220644..2221729 (-) 1086 WP_122955353.1 Obg family GTPase CgtA -
  DT070_RS10530 (DT070_10525) rpmA 2221847..2222104 (-) 258 WP_122955354.1 50S ribosomal protein L27 -
  DT070_RS10535 (DT070_10530) rplU 2222132..2222443 (-) 312 WP_122955355.1 50S ribosomal protein L21 -
  DT070_RS10540 (DT070_10535) - 2222640..2223623 (+) 984 WP_122955356.1 polyprenyl synthetase family protein -
  DT070_RS10550 (DT070_10545) pilB 2223848..2225581 (+) 1734 WP_122955357.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DT070_RS10555 (DT070_10550) pilC 2225666..2226883 (+) 1218 WP_122955358.1 type II secretion system F family protein Machinery gene
  DT070_RS10560 (DT070_10555) - 2226883..2227743 (+) 861 WP_122955359.1 A24 family peptidase -
  DT070_RS10565 (DT070_10560) coaE 2227748..2228359 (+) 612 WP_122955360.1 dephospho-CoA kinase -
  DT070_RS10570 (DT070_10565) zapD 2228403..2229158 (+) 756 WP_122957352.1 cell division protein ZapD -
  DT070_RS10575 (DT070_10570) - 2229205..2229426 (+) 222 WP_122955361.1 DNA gyrase inhibitor YacG -
  DT070_RS10580 (DT070_10575) - 2229540..2229827 (+) 288 WP_122955362.1 hypothetical protein -
  DT070_RS10585 (DT070_10580) grxD 2229862..2230191 (-) 330 WP_122955363.1 Grx4 family monothiol glutaredoxin -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62614.12 Da        Isoelectric Point: 5.1505

>NTDB_id=302867 DT070_RS10550 WP_122955357.1 2223848..2225581(+) (pilB) [Polaromonas sp. SP1]
MAAVDTAINQPPSMALPGLGRALVMAGKLGQKAAEDIFRKAQSSRTSFIAELTGSGAVSAFDLAHTMSTAFAAPLLDLEA
IDVQRLPKGLLDTKICATYRIVVLSKRNNRLIVATADPSDQEAAEKIKFSTQMGVDWVIAEYDKLSKLVEASNTTAAEAM
ESIIGDDFEFDESSTEASAEDSEASVSEVEDAPVVKFLQKMLLDAFSMRASDLHFEPFEHTYRVRFRVDGELREIASPPV
AIKEKLAARIKVISKLDISEKRVPQDGKMKLKIGPDRVIDFRVSTLPTMYGEKIVIRILDPSSAKLGIDALGYEADEKER
LLHAIQRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGVNIATAEDPAEITLPGVNQVSMNDKAGMTFSVALKAFLRQDPD
IIMVGEIRDLETADIAIKAAQTGHMVMSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPNCKTPADIPR
ETLLDAGFKEEDVDGSWTPYRPVGCSMCTNGYKGRVGVYQLMPISEEIQRIVLRDGSALEIAAQAEAEGVKSLRVSGLSK
VKLGMTSLEEILGCTNV

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=302867 DT070_RS10550 WP_122955357.1 2223848..2225581(+) (pilB) [Polaromonas sp. SP1]
ATGGCCGCTGTCGACACCGCTATCAACCAACCGCCGTCCATGGCTTTACCCGGCTTGGGCCGAGCCCTGGTGATGGCCGG
AAAATTGGGGCAGAAAGCTGCCGAAGACATTTTTCGCAAGGCCCAAAGCAGCCGTACCAGCTTTATCGCCGAATTGACGG
GCTCCGGCGCGGTATCCGCTTTTGATCTGGCCCACACCATGTCGACGGCTTTCGCTGCCCCTTTGCTGGATCTGGAAGCC
ATCGACGTACAGCGCCTGCCCAAAGGCCTGCTGGACACCAAAATCTGCGCCACCTACCGAATTGTTGTCCTGAGCAAGCG
CAACAACCGTCTGATCGTCGCGACTGCAGATCCTTCCGACCAGGAGGCCGCGGAGAAGATCAAGTTTTCCACCCAGATGG
GGGTGGATTGGGTCATTGCGGAGTACGACAAGCTGTCCAAGCTGGTAGAGGCGTCCAACACCACGGCCGCCGAGGCCATG
GAAAGCATCATCGGTGACGACTTCGAGTTTGACGAATCGAGCACCGAGGCCTCTGCCGAAGACAGTGAAGCCAGCGTTTC
AGAAGTCGAGGACGCGCCCGTCGTCAAATTTCTGCAAAAAATGCTGCTTGACGCTTTCAGCATGCGGGCTTCCGACCTGC
ATTTCGAGCCGTTTGAGCATACCTACCGTGTCCGGTTCCGGGTTGACGGAGAACTGCGCGAAATCGCATCGCCACCGGTG
GCCATCAAGGAAAAGCTCGCGGCGCGCATCAAGGTGATCTCAAAGCTGGACATCTCGGAAAAGCGCGTGCCGCAGGACGG
CAAGATGAAGCTCAAGATAGGCCCGGACCGGGTCATCGATTTCCGGGTCAGCACCTTGCCCACCATGTATGGCGAGAAAA
TCGTCATCCGTATTCTTGACCCGAGCAGTGCCAAGTTGGGCATTGATGCCCTGGGCTACGAGGCGGATGAGAAGGAGCGC
TTGCTCCACGCGATTCAGCGGCCCTATGGCATGGTGCTGGTGACCGGGCCCACCGGTTCGGGCAAGACGGTGTCGCTTTA
CACCTGCCTGAACATTCTCAACAAGCCGGGCGTCAACATCGCTACTGCCGAGGATCCCGCCGAAATTACCTTGCCTGGCG
TCAACCAGGTCAGCATGAACGACAAGGCCGGCATGACCTTTTCAGTCGCGCTCAAGGCGTTCTTGCGGCAGGATCCCGAC
ATCATCATGGTCGGTGAGATCCGGGATCTTGAGACCGCCGATATCGCGATCAAGGCCGCGCAGACCGGCCATATGGTGAT
GTCGACGTTGCACACCAACGATGCACCGACCACACTGACGCGTATGCGCAACATGGGCATTGCACCTTTCAATATCGCCT
CCAGCGTGATTCTGATCACCGCTCAGCGTTTGGCCCGCCGCCTTTGCCCCAATTGCAAGACGCCTGCGGACATCCCGCGT
GAGACCCTGCTTGACGCGGGCTTCAAGGAAGAGGATGTAGACGGCTCATGGACGCCTTACCGCCCGGTAGGCTGCTCGAT
GTGTACGAATGGTTACAAAGGGCGGGTGGGCGTTTATCAGCTCATGCCTATTTCCGAGGAGATCCAGCGTATCGTCCTTC
GCGACGGCAGCGCCCTGGAAATTGCTGCCCAGGCGGAGGCTGAGGGCGTGAAAAGCCTGCGGGTGTCGGGTTTGTCCAAA
GTTAAACTCGGCATGACTTCGCTGGAAGAGATTCTGGGCTGTACCAATGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q3HLQ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.557

98.267

0.516
  pilB Acinetobacter baylyi ADP1

51.675

98.267

0.508
  pilF Neisseria gonorrhoeae MS11

50.534

97.4

0.492
  pilB Legionella pneumophila strain ERS1305867

49.286

97.054

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

43.21

98.267

0.425
  pilB Vibrio campbellii strain DS40M4

43.316

97.227

0.421
  pilB Vibrio cholerae strain A1552

45.857

89.948

0.412
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.265

91.681

0.369
  pilF Thermus thermophilus HB27

37.3

97.574

0.364

Multiple sequence alignment