Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CSC44_RS20270 Genome accession   NZ_CP030327
Coordinates   4258147..4259364 (-) Length   405 a.a.
NCBI ID   WP_023103506.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain AR_458     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4253147..4264364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSC44_RS20225 (CSC44_4083) - 4253329..4253778 (-) 450 WP_003161439.1 MOSC domain-containing protein -
  CSC44_RS20230 (CSC44_4084) - 4253775..4254404 (-) 630 WP_003094668.1 DUF1780 domain-containing protein -
  CSC44_RS20235 (CSC44_4085) - 4254537..4254962 (+) 426 WP_016562341.1 GNAT family N-acetyltransferase -
  CSC44_RS20240 (CSC44_4086) - 4254959..4255429 (+) 471 WP_023103507.1 hypothetical protein -
  CSC44_RS20245 (CSC44_4087) - 4255426..4256115 (+) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  CSC44_RS20250 (CSC44_4088) - 4256221..4256430 (+) 210 WP_003094660.1 hypothetical protein -
  CSC44_RS20255 (CSC44_4089) yacG 4256467..4256667 (-) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  CSC44_RS20260 (CSC44_4090) coaE 4256664..4257275 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  CSC44_RS20265 (CSC44_4091) pilD 4257272..4258144 (-) 873 WP_017148550.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CSC44_RS20270 (CSC44_4092) pilC 4258147..4259364 (-) 1218 WP_023103506.1 type II secretion system F family protein Machinery gene
  CSC44_RS20275 (CSC44_4093) pilB 4259368..4261071 (-) 1704 WP_023100230.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CSC44_RS20280 pilE 4261298..4261834 (+) 537 WP_025921208.1 pilin Machinery gene
  CSC44_RS31895 tfpZ 4261899..4262636 (+) 738 WP_023110409.1 TfpX/TfpZ family type IV pilin accessory protein -
  CSC44_RS20290 (CSC44_4095) nadC 4262870..4263718 (-) 849 WP_017148538.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43875.86 Da        Isoelectric Point: 9.9457

>NTDB_id=300888 CSC44_RS20270 WP_023103506.1 4258147..4259364(-) (pilC) [Pseudomonas aeruginosa strain AR_458]
MADKALKTSVFIWEGTDKKGSKVKGELTGQNPMLVKAQLRKQGINPLKVRKKGITLFGAGKKIKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFENPNMRKLVDEIKQEVAAGNSLANSLRKKPQYFDDLYCNLVDAGEQSGALETLLARVATYKEKT
EALKAKIKKAMTYPIAVVIVALVVSAILLIKVVPQFQSVFANFGAELPAFTQMVIGLSETLQEWWLLVLIAMLGIGFALK
ELYKRSKSFRDTIDRAILKAPIVGAIVYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKSAVAKIKQDVSSGM
QLNFSMRTTNVFPSMAIQMAAIGEESGSLDDMLGKVAGFYEDEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGKVI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=300888 CSC44_RS20270 WP_023103506.1 4258147..4259364(-) (pilC) [Pseudomonas aeruginosa strain AR_458]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCATCTGGGAAGGTACTGATAAGAAGGGCAGCAAGGTCAAAGGAGAGTT
GACCGGGCAAAACCCTATGCTGGTCAAAGCCCAGCTACGCAAACAGGGTATCAATCCGCTGAAAGTCCGCAAGAAAGGCA
TCACGCTATTCGGTGCGGGAAAAAAAATCAAACCCATGGACATCGCACTGTTCACTCGGCAGATGGCGACCATGATGGGC
GCAGGCGTGCCCCTATTGCAGTCCTTCGACATCATTGGCGAGGGCTTCGAAAATCCGAACATGCGAAAGCTGGTTGACGA
AATAAAGCAGGAAGTCGCAGCCGGTAATAGCTTGGCCAACTCACTACGAAAGAAGCCCCAGTATTTTGACGACCTTTACT
GCAATCTTGTAGATGCGGGCGAACAATCAGGCGCATTGGAAACGCTTCTCGCCCGGGTTGCTACCTACAAGGAAAAAACT
GAGGCACTCAAAGCCAAGATCAAGAAAGCTATGACCTACCCCATCGCGGTCGTTATCGTGGCACTGGTGGTCTCTGCGAT
CCTACTCATTAAGGTTGTTCCGCAATTCCAGTCAGTTTTCGCGAACTTTGGTGCAGAGCTACCAGCCTTCACTCAAATGG
TGATCGGACTTTCGGAAACATTACAAGAATGGTGGCTACTTGTCCTCATCGCCATGCTCGGCATAGGTTTTGCTCTGAAA
GAGTTGTACAAGCGCTCCAAAAGTTTCCGCGACACCATAGACAGAGCCATACTGAAAGCTCCCATCGTCGGTGCCATCGT
CTATAAGTCAGCCGTAGCACGTTATGCACGGACCCTATCGACTACGTTTGCAGCAGGCGTTCCTCTAGTCGAGGCTTTGG
ATTCGGTATCAGGGGCCACAGGTAATGTGGTGTTTAAAAGCGCAGTCGCCAAGATCAAGCAGGACGTTTCCAGCGGTATG
CAGTTGAACTTCTCCATGCGCACCACCAATGTTTTTCCGAGTATGGCTATCCAGATGGCTGCGATTGGCGAAGAATCTGG
TTCGCTGGACGACATGCTTGGCAAAGTGGCTGGCTTCTACGAGGATGAAGTAGATAATGCCGTCGACAATTTGACGACTT
TGATGGAACCAATGATCATGGCGGTGCTGGGAGTCCTGGTTGGCGGATTGATCGTCGCTATGTATCTGCCTATTTTCCAG
CTCGGCAAAGTTATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.506

100

0.795
  pilC Acinetobacter baylyi ADP1

58.968

100

0.593
  pilC Acinetobacter baumannii D1279779

59.158

99.753

0.59
  pilC Legionella pneumophila strain ERS1305867

53.535

97.778

0.523
  pilG Neisseria meningitidis 44/76-A

45.926

100

0.459
  pilG Neisseria gonorrhoeae MS11

45.679

100

0.457
  pilC Vibrio cholerae strain A1552

42.963

100

0.43
  pilC Vibrio campbellii strain DS40M4

41.414

97.778

0.405
  pilC Thermus thermophilus HB27

37.406

99.012

0.37

Multiple sequence alignment