Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CT138_RS06410 Genome accession   NZ_CP030062
Coordinates   1307816..1309207 (+) Length   463 a.a.
NCBI ID   WP_112110821.1    Uniprot ID   -
Organism   Mannheimia varigena strain Teagasc     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1302816..1314207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CT138_RS06385 (CT138_06385) - 1303220..1303915 (+) 696 WP_025247567.1 aspartate/glutamate racemase family protein -
  CT138_RS06390 (CT138_06390) - 1303933..1305243 (+) 1311 WP_112110818.1 anaerobic C4-dicarboxylate transporter -
  CT138_RS06395 (CT138_06395) - 1305470..1305703 (+) 234 WP_025247569.1 YgjV family protein -
  CT138_RS06400 (CT138_06400) radA 1305757..1307136 (-) 1380 WP_112110819.1 DNA repair protein RadA -
  CT138_RS06405 (CT138_06405) pilA 1307316..1307795 (+) 480 WP_112110820.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CT138_RS06410 (CT138_06410) pilB 1307816..1309207 (+) 1392 WP_112110821.1 GspE/PulE family protein Machinery gene
  CT138_RS06415 (CT138_06415) pilC 1309200..1310396 (+) 1197 WP_112110822.1 type II secretion system F family protein Machinery gene
  CT138_RS06420 (CT138_06420) - 1310607..1311056 (+) 450 WP_239493893.1 prepilin peptidase -
  CT138_RS06425 (CT138_06425) coaE 1311088..1311723 (+) 636 WP_112110824.1 dephospho-CoA kinase -
  CT138_RS06430 (CT138_06430) yacG 1311705..1311890 (+) 186 WP_025217069.1 DNA gyrase inhibitor YacG -
  CT138_RS06435 (CT138_06435) holA 1311891..1312913 (-) 1023 WP_112110825.1 DNA polymerase III subunit delta -
  CT138_RS06440 (CT138_06440) lptE 1312953..1313441 (-) 489 WP_112110826.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52389.10 Da        Isoelectric Point: 5.3684

>NTDB_id=298828 CT138_RS06410 WP_112110821.1 1307816..1309207(+) (pilB) [Mannheimia varigena strain Teagasc]
MEYTVTDLTSQEMIEISGEQWAKNCNEKQILLRYLAVPLQETQSQLWLAVDDMQNISACEIFAFIYHKQIEPVLISSDEL
KFLLNALSPEQSSLQSEIYEDNVAYSLAHSEQIDRNDPIIQILDNLFKFCLKNNASDIHLEPQKEKLLIRLRVDGVLYIY
KTLPQSLSSRLISRIKLLAKLDISEIRLPQDGQFYFKTMLAETLDFRVSTLPTHFGEKVVLRLQKNKPTNLDFLELGFNQ
NQKETLLNAINQPQGLILVTGPTGSGKSITLYSALSHLNTSEKHILTAEDPIGIEIEGIIQTQVNRGINLDFSQLLRTFL
RQDPDIIMLGEIRDEESAEIALRASQTGHLVLSTLHTNDAPSAIERLLQLGIKEYELQNALLLVVAQRLLRRICTKCRGA
GCEHCYQGYKGRIGVYQLFSRTAKNFEKTTACLDFATLSESAKEKLAEGKTDQAEIFRVLGNG

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=298828 CT138_RS06410 WP_112110821.1 1307816..1309207(+) (pilB) [Mannheimia varigena strain Teagasc]
ATGGAGTATACAGTTACAGATTTAACTAGCCAAGAAATGATTGAAATTTCTGGTGAGCAATGGGCAAAAAATTGTAATGA
AAAGCAGATTTTATTGCGATATTTAGCCGTTCCGTTGCAAGAAACACAATCTCAATTATGGTTGGCGGTGGATGATATGC
AAAATATCAGTGCTTGTGAGATTTTTGCCTTTATCTACCATAAGCAAATTGAACCTGTTTTGATTTCCTCCGATGAGTTG
AAGTTTCTGCTCAATGCGCTTTCACCAGAGCAGAGTAGCTTGCAGAGTGAAATTTATGAGGATAATGTAGCGTATTCATT
AGCACATTCGGAGCAAATAGACAGAAACGATCCGATTATCCAAATTTTAGATAATCTGTTTAAATTCTGTTTAAAAAATA
ACGCATCAGATATTCATCTTGAACCGCAAAAAGAGAAGTTGCTTATTCGGTTAAGAGTTGACGGTGTATTATATATTTAT
AAAACACTTCCGCAAAGTTTATCTAGCAGGTTGATTTCTCGCATTAAGCTGTTAGCTAAATTAGATATTAGCGAAATCCG
TTTGCCACAAGATGGACAATTTTATTTCAAAACGATGTTAGCGGAAACTTTAGATTTCCGAGTTTCTACTTTGCCTACTC
ATTTTGGCGAAAAGGTCGTCCTTCGGCTCCAAAAGAATAAACCGACTAATTTGGATTTTTTGGAGTTAGGTTTTAACCAA
AACCAAAAAGAAACATTACTCAATGCAATCAACCAACCTCAAGGATTAATTCTGGTAACTGGTCCAACAGGGAGTGGAAA
AAGTATCACTTTATATAGTGCATTAAGCCATCTCAATACTAGCGAAAAGCATATATTAACCGCTGAAGATCCGATTGGAA
TTGAGATAGAAGGTATTATTCAAACCCAAGTAAACAGGGGCATTAATCTTGATTTTAGCCAACTGTTGAGAACCTTTCTC
CGCCAAGATCCCGACATCATTATGCTTGGTGAAATTCGTGATGAAGAGAGTGCTGAAATTGCCCTAAGAGCATCTCAAAC
AGGGCATTTAGTGCTTTCTACTTTGCACACAAACGATGCTCCTTCTGCCATAGAACGATTATTGCAACTCGGAATTAAAG
AATATGAGTTACAAAATGCGTTATTGTTAGTAGTAGCTCAACGCTTGTTAAGAAGGATTTGTACAAAATGCAGAGGGGCT
GGTTGTGAGCATTGCTATCAAGGCTACAAAGGGCGAATTGGGGTGTATCAACTTTTTAGCCGAACTGCAAAAAATTTTGA
AAAAACGACCGCTTGTTTAGATTTTGCAACCTTATCGGAAAGTGCAAAAGAAAAATTAGCGGAAGGAAAAACCGATCAGG
CAGAAATTTTTAGGGTGTTAGGAAATGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(131-392)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

71.584

99.568

0.713
  pilB Haemophilus influenzae 86-028NP

56.291

97.84

0.551
  pilB Haemophilus influenzae Rd KW20

56.195

97.624

0.549
  pilB Vibrio cholerae strain A1552

39.96

100

0.43
  pilB Vibrio parahaemolyticus RIMD 2210633

39.96

100

0.43
  pilB Vibrio campbellii strain DS40M4

39.959

100

0.425
  pilB Legionella pneumophila strain ERS1305867

39.344

100

0.415
  pilB Acinetobacter baylyi ADP1

36.293

100

0.406
  pilB Acinetobacter baumannii D1279779

36.605

100

0.387
  ctsE Campylobacter jejuni subsp. jejuni 81-176

40.767

90.065

0.367
  pilF Neisseria gonorrhoeae MS11

39.765

91.793

0.365

Multiple sequence alignment