Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   DKY63_RS15605 Genome accession   NZ_CP029693
Coordinates   3357226..3358443 (+) Length   405 a.a.
NCBI ID   WP_110964921.1    Uniprot ID   A0A2Z4RJW4
Organism   Pseudomonas putida strain JBC17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3352226..3363443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKY63_RS15585 (DKY63_15585) - 3353672..3353968 (+) 297 WP_110964917.1 DUF2845 domain-containing protein -
  DKY63_RS15590 (DKY63_15590) - 3354237..3354590 (-) 354 WP_110964918.1 BON domain-containing protein -
  DKY63_RS15595 (DKY63_15595) - 3354900..3355301 (-) 402 WP_110964919.1 pilin -
  DKY63_RS15600 (DKY63_15600) pilB 3355529..3357223 (+) 1695 WP_110964920.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DKY63_RS15605 (DKY63_15605) pilC 3357226..3358443 (+) 1218 WP_110964921.1 type II secretion system F family protein Machinery gene
  DKY63_RS15610 (DKY63_15610) pilD 3358445..3359317 (+) 873 WP_110964922.1 A24 family peptidase Machinery gene
  DKY63_RS15615 (DKY63_15615) coaE 3359314..3359937 (+) 624 WP_110964923.1 dephospho-CoA kinase -
  DKY63_RS15620 (DKY63_15620) yacG 3359934..3360137 (+) 204 WP_110964924.1 DNA gyrase inhibitor YacG -
  DKY63_RS15625 (DKY63_15625) - 3360168..3360857 (-) 690 WP_110964925.1 energy-coupling factor ABC transporter permease -
  DKY63_RS15630 (DKY63_15630) - 3361118..3361357 (+) 240 WP_110964926.1 DUF2790 domain-containing protein -
  DKY63_RS15635 (DKY63_15635) - 3361366..3362634 (-) 1269 Protein_3007 OprD family porin -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43851.65 Da        Isoelectric Point: 9.9678

>NTDB_id=295373 DKY63_RS15605 WP_110964921.1 3357226..3358443(+) (pilC) [Pseudomonas putida strain JBC17]
MAVKAAKISTYAWEGTDRKGTKVTGELSGQNPALIKAQLRKQGINPGRVRKKTPSLLNLGKGIKAKDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRTLVDEVKQEVAAGNSFAAALRKKPKYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPLAVIFVATIVTGILLVKVVPQFQSVFASFGAELPAFTVMVIGLSHFMQDWWWAMLGGLIAAILGMR
HGLKTSQALRDRVDAWLLKVPLVGALLYKSAVARFARTLSTTFAAGVPLVEALDSVAGAAGNVVFKRAVLRIRQDVSTGM
QLNFSMRTTGLFPNMAIQMTAIGEESGALDEMLDKVAGFYEDEVDNMVDSLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSTI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=295373 DKY63_RS15605 WP_110964921.1 3357226..3358443(+) (pilC) [Pseudomonas putida strain JBC17]
ATGGCGGTCAAAGCGGCAAAAATCAGCACCTACGCCTGGGAAGGCACCGACCGCAAAGGTACGAAAGTCACCGGCGAGCT
GAGCGGCCAGAACCCGGCCCTAATCAAGGCGCAACTGCGCAAGCAAGGCATCAACCCCGGCCGGGTGCGCAAGAAAACGC
CCTCCCTGCTCAACCTGGGCAAGGGCATCAAGGCCAAGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAA
GCCGGCGTGCCCTTGCTGCAGTCGTTCGACATCATCGGCGAAGGCTTCGACAACCCGGCCATGCGCACGCTGGTAGATGA
GGTCAAACAGGAAGTCGCCGCTGGCAACAGCTTCGCCGCAGCCCTGCGCAAAAAGCCCAAATACTTCGATGAGTTGTATT
GCAACCTGGTGGACGCCGGCGAGCAGGCCGGCGCCCTGGATACCCTGCTGGAGCGGGTGGCGACCTATAAGGAAAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCGCTGGCGGTGATATTCGTCGCCACCATCGTGACGGGGAT
TCTGCTGGTCAAAGTGGTGCCGCAATTTCAGTCGGTGTTCGCAAGCTTTGGTGCTGAATTGCCGGCATTTACCGTGATGG
TCATCGGCCTGTCGCACTTCATGCAGGACTGGTGGTGGGCGATGCTCGGCGGCTTGATCGCGGCGATCCTGGGCATGCGC
CACGGGCTCAAGACGTCGCAGGCTTTGCGTGATCGAGTGGATGCGTGGCTGCTGAAAGTACCCCTTGTAGGCGCCCTGCT
GTACAAGTCCGCCGTGGCCCGTTTTGCCCGCACTCTGTCGACCACCTTTGCCGCTGGCGTACCCCTTGTCGAGGCCCTGG
ATTCCGTGGCGGGCGCCGCCGGTAATGTGGTGTTCAAGCGCGCCGTGCTGCGCATCCGGCAGGACGTCTCGACGGGCATG
CAATTGAATTTCTCCATGCGCACTACCGGGCTTTTTCCGAACATGGCGATCCAGATGACCGCCATTGGCGAAGAGTCCGG
CGCACTGGACGAGATGCTGGACAAGGTCGCGGGTTTCTATGAAGACGAAGTCGACAATATGGTCGACAGCCTCACCAGCC
TGATGGAACCGTTCATCATGGTGGTACTGGGTGTTATTGTCGGTGGCCTGGTGGTTGCGATGTACCTGCCCATCTTCCAA
CTCGGGTCGACGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z4RJW4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.333

100

0.733
  pilC Acinetobacter baylyi ADP1

58.824

100

0.593
  pilC Acinetobacter baumannii D1279779

58.824

100

0.593
  pilC Legionella pneumophila strain ERS1305867

52.141

98.025

0.511
  pilG Neisseria gonorrhoeae MS11

45.161

99.506

0.449
  pilG Neisseria meningitidis 44/76-A

44.665

99.506

0.444
  pilC Vibrio cholerae strain A1552

42.222

100

0.422
  pilC Vibrio campbellii strain DS40M4

39.409

100

0.395
  pilC Thermus thermophilus HB27

38.806

99.259

0.385

Multiple sequence alignment