Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   DLD99_RS24540 Genome accession   NZ_CP029608
Coordinates   5380096..5381313 (+) Length   405 a.a.
NCBI ID   WP_114885597.1    Uniprot ID   -
Organism   Pseudomonas kribbensis strain 46-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5375096..5386313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DLD99_RS24520 (DLD99_24515) - 5376601..5376897 (+) 297 WP_114885591.1 DUF2845 domain-containing protein -
  DLD99_RS24525 (DLD99_24520) - 5377067..5377423 (-) 357 WP_065261581.1 BON domain-containing protein -
  DLD99_RS24530 (DLD99_24525) - 5377723..5378166 (-) 444 WP_114885593.1 pilin -
  DLD99_RS24535 (DLD99_24530) pilB 5378393..5380093 (+) 1701 WP_114885595.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DLD99_RS24540 (DLD99_24535) pilC 5380096..5381313 (+) 1218 WP_114885597.1 type II secretion system F family protein Machinery gene
  DLD99_RS24545 (DLD99_24540) pilD 5381315..5382187 (+) 873 WP_085712165.1 A24 family peptidase Machinery gene
  DLD99_RS24550 (DLD99_24545) coaE 5382184..5382807 (+) 624 WP_114885599.1 dephospho-CoA kinase -
  DLD99_RS24555 (DLD99_24550) yacG 5382804..5383004 (+) 201 WP_085712167.1 DNA gyrase inhibitor YacG -
  DLD99_RS24560 (DLD99_24555) - 5383010..5383225 (-) 216 WP_096820888.1 hypothetical protein -
  DLD99_RS24565 (DLD99_24560) - 5383291..5383980 (-) 690 WP_085712168.1 energy-coupling factor ABC transporter permease -
  DLD99_RS24570 (DLD99_24565) - 5384114..5384740 (+) 627 WP_065261573.1 DUF1780 domain-containing protein -
  DLD99_RS24575 (DLD99_24570) - 5384885..5385058 (+) 174 WP_065261571.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44089.94 Da        Isoelectric Point: 10.1093

>NTDB_id=294074 DLD99_RS24540 WP_114885597.1 5380096..5381313(+) (pilC) [Pseudomonas kribbensis strain 46-2]
MAVKAAKISVYAWEGTDKKGSRISGELSGQNPALIKAQLRKQGINPGKVRKKSASLLNFGKRIKPLDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
ESLKAKIKKAMTYPTAVILVAAVVTGILLVKVVPQFQSVFSGFGAELPAFTLMVIGLSEFMQQWWWAILGVLIVAFFGTR
HALKKSPALRDRRDAWLLKLPLVGTMMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVATGM
QLNFSMRTSGIFPNMAVQMTAIGEESGALDDMLDKVAGFYEEEVDNMVDNLTSLMEPFVMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=294074 DLD99_RS24540 WP_114885597.1 5380096..5381313(+) (pilC) [Pseudomonas kribbensis strain 46-2]
ATGGCCGTCAAGGCAGCGAAAATCAGCGTCTACGCCTGGGAAGGCACCGACAAGAAAGGCAGCCGGATCAGCGGCGAACT
GAGCGGACAAAACCCCGCGCTGATCAAGGCCCAGTTGCGCAAGCAGGGCATCAACCCCGGCAAGGTGCGCAAGAAATCCG
CGTCGTTGCTGAATTTCGGCAAACGCATCAAGCCGCTGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGTTGCTGCAGTCATTCGACATCATCGGCGAAGGCTTCGAGAACCCGGCGATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAAGTCGCCGCCGGTAACAGCTTCGCCGCGGCCCTGCGCAAGAAGCCGCAATACTTCGACGAGTTGTATT
GCAACCTGGTCGATGCCGGCGAGCAGTCCGGTGCTCTCGACACCCTGCTCGAGCGCGTGGCGACCTACAAGGAGAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCCACCGCCGTGATTCTGGTGGCGGCGGTCGTCACCGGCAT
TCTGCTGGTGAAAGTGGTGCCGCAGTTCCAGTCGGTATTTTCCGGCTTCGGTGCCGAACTGCCGGCGTTCACCCTGATGG
TGATCGGTCTGTCGGAGTTCATGCAGCAATGGTGGTGGGCGATTCTGGGTGTGCTGATCGTGGCGTTTTTCGGCACCCGC
CATGCCTTGAAGAAATCCCCGGCCCTGCGTGACCGACGTGATGCCTGGCTGCTGAAACTGCCACTGGTGGGCACCATGAT
GTACAAGTCCGCCGTCGCCCGTTTTGCCCGGACCCTGTCGACCACGTTTGCCGCCGGCGTGCCGCTGGTCGAAGCGCTGG
ACTCGGTCGCCGGAGCCACCGGCAACGTGGTGTTCAAGCGCGCGGTGCTGCGCATTCGCCAGGACGTGGCCACCGGCATG
CAGTTGAATTTCTCGATGCGCACCAGCGGAATCTTTCCGAACATGGCCGTGCAGATGACCGCCATCGGCGAAGAATCCGG
CGCCCTGGACGACATGCTCGACAAGGTGGCCGGGTTCTACGAGGAAGAAGTGGATAACATGGTCGACAACCTCACCAGTC
TGATGGAGCCTTTCGTCATGGTGGTACTCGGGGTGATCGTCGGCGGGCTGGTCGTGGCCATGTACTTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.58

100

0.736
  pilC Acinetobacter baumannii D1279779

59.069

100

0.595
  pilC Acinetobacter baylyi ADP1

58.824

100

0.593
  pilC Legionella pneumophila strain ERS1305867

53.25

98.765

0.526
  pilG Neisseria gonorrhoeae MS11

42.68

99.506

0.425
  pilC Vibrio cholerae strain A1552

43.182

97.778

0.422
  pilG Neisseria meningitidis 44/76-A

42.184

99.506

0.42
  pilC Vibrio campbellii strain DS40M4

41.032

100

0.412
  pilC Thermus thermophilus HB27

38.404

99.012

0.38

Multiple sequence alignment