Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DK843_RS14395 Genome accession   NZ_CP029554
Coordinates   2981251..2982387 (+) Length   378 a.a.
NCBI ID   WP_114061129.1    Uniprot ID   A0A344UJD2
Organism   Chromobacterium phragmitis strain IIBBL 274-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2976251..2987387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DK843_RS14365 (DK843_14365) dksA 2976790..2977200 (-) 411 WP_011133729.1 RNA polymerase-binding protein DksA -
  DK843_RS14370 (DK843_14370) - 2977486..2977791 (-) 306 WP_114061125.1 c-type cytochrome -
  DK843_RS14375 (DK843_14375) - 2977873..2978454 (-) 582 WP_114061126.1 YggT family protein -
  DK843_RS14380 (DK843_14380) proC 2978464..2979267 (-) 804 WP_114061127.1 pyrroline-5-carboxylate reductase -
  DK843_RS14385 (DK843_14385) - 2979321..2980016 (-) 696 WP_114073602.1 YggS family pyridoxal phosphate-dependent enzyme -
  DK843_RS14390 (DK843_14390) pilT 2980148..2981185 (+) 1038 WP_114063686.1 type IV pilus twitching motility protein PilT Machinery gene
  DK843_RS14395 (DK843_14395) pilU 2981251..2982387 (+) 1137 WP_114061129.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DK843_RS14400 (DK843_14400) - 2982416..2984029 (+) 1614 WP_114073603.1 response regulator -
  DK843_RS14405 (DK843_14405) - 2984067..2985680 (+) 1614 WP_114073604.1 response regulator -
  DK843_RS14410 (DK843_14410) thpR 2985685..2986236 (+) 552 WP_114073605.1 RNA 2',3'-cyclic phosphodiesterase -
  DK843_RS14415 (DK843_14415) - 2986217..2986948 (-) 732 WP_114061133.1 glycoside hydrolase family 16 protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42326.55 Da        Isoelectric Point: 6.1405

>NTDB_id=293470 DK843_RS14395 WP_114061129.1 2981251..2982387(+) (pilU) [Chromobacterium phragmitis strain IIBBL 274-1]
MEKDQASKFIHDLLKHAVGKNASDIFIAAEFPPAMKIDGKITPVAPQPLSALQSKELVRAVMNDRQTEEFEAKKEANFAI
NPPGVGRFRVSAFVQQGMVGMVLRKINTEIPTFDHLNLPEVLKDIALIKRGLVIFVGGTGSGKSTSLAALVDWRNSNNQD
HIITIEDPIEYVHTHKKSIVTQREIGVDTENWEIALKNTLRQAPDVILMGEIRDRETMAYGLQFAETGHLCLATLHANNA
NQALDRILNFFPEERHQQVLMDLSLNMRAIISQRLVPHKSGRGRVAAVEILLNSPLVSDMVFKGEIAGLKEVMSRSREAG
MQTFDQSLFELYEADLITYEDALRNADSINDLRLKIKLYSESARNRDPLEGIDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=293470 DK843_RS14395 WP_114061129.1 2981251..2982387(+) (pilU) [Chromobacterium phragmitis strain IIBBL 274-1]
ATGGAAAAAGACCAGGCCTCCAAGTTCATCCACGACCTGCTCAAGCATGCGGTCGGCAAGAACGCCTCCGACATCTTCAT
CGCCGCCGAATTTCCGCCGGCGATGAAGATAGACGGCAAGATCACCCCGGTGGCGCCCCAGCCTTTGAGCGCGCTGCAAA
GCAAGGAGCTGGTGCGGGCGGTGATGAACGACCGGCAGACCGAGGAGTTCGAAGCGAAGAAAGAAGCCAATTTCGCGATC
AACCCGCCCGGCGTCGGCCGCTTCCGCGTCAGCGCCTTCGTGCAGCAGGGCATGGTGGGCATGGTGCTGCGCAAGATCAA
CACCGAGATTCCCACCTTCGACCATCTGAATCTGCCCGAGGTGCTGAAGGACATCGCGCTGATCAAGCGCGGACTGGTGA
TCTTCGTCGGCGGCACGGGCTCCGGCAAGTCCACTTCGCTGGCGGCGCTGGTGGACTGGCGCAACAGCAACAATCAGGAC
CACATCATCACGATCGAGGACCCGATCGAGTATGTGCACACCCATAAGAAGTCCATCGTCACCCAGCGCGAGATCGGCGT
CGATACCGAGAATTGGGAGATCGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAAACCATGGCCTACGGCCTGCAGTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCC
AACCAGGCGCTGGACCGCATCCTCAACTTCTTCCCCGAGGAGCGCCACCAGCAGGTGCTGATGGACCTGTCGCTGAACAT
GCGCGCCATCATTTCGCAGCGGCTGGTGCCGCATAAATCCGGACGCGGACGGGTGGCGGCGGTGGAGATCCTGCTCAACA
GCCCGCTGGTGTCGGACATGGTATTCAAGGGCGAGATCGCCGGCCTGAAGGAGGTGATGTCGCGTTCGCGCGAGGCCGGC
ATGCAAACCTTCGACCAGTCGCTGTTCGAACTGTACGAGGCGGACTTGATCACTTACGAGGATGCGTTGCGCAATGCGGA
CTCCATCAATGACCTGCGCTTGAAAATCAAGCTGTACAGCGAAAGCGCGCGCAATCGCGATCCGCTGGAAGGCATAGACC
ACCTGGACATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A344UJD2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.429

92.593

0.569
  pilU Acinetobacter baylyi ADP1

55.191

96.825

0.534
  pilU Vibrio cholerae strain A1552

52.991

92.857

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Acinetobacter baumannii strain A118

41.369

88.889

0.368
  pilT Acinetobacter nosocomialis M2

41.369

88.889

0.368
  pilT Acinetobacter baumannii D1279779

41.369

88.889

0.368
  pilT Pseudomonas aeruginosa PAK

40.95

89.153

0.365

Multiple sequence alignment