Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DK843_RS14390 Genome accession   NZ_CP029554
Coordinates   2980148..2981185 (+) Length   345 a.a.
NCBI ID   WP_114063686.1    Uniprot ID   A0A344UP30
Organism   Chromobacterium phragmitis strain IIBBL 274-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2975148..2986185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DK843_RS14360 (DK843_14360) aroG 2975596..2976657 (-) 1062 WP_114061124.1 3-deoxy-7-phosphoheptulonate synthase AroG -
  DK843_RS14365 (DK843_14365) dksA 2976790..2977200 (-) 411 WP_011133729.1 RNA polymerase-binding protein DksA -
  DK843_RS14370 (DK843_14370) - 2977486..2977791 (-) 306 WP_114061125.1 c-type cytochrome -
  DK843_RS14375 (DK843_14375) - 2977873..2978454 (-) 582 WP_114061126.1 YggT family protein -
  DK843_RS14380 (DK843_14380) proC 2978464..2979267 (-) 804 WP_114061127.1 pyrroline-5-carboxylate reductase -
  DK843_RS14385 (DK843_14385) - 2979321..2980016 (-) 696 WP_114073602.1 YggS family pyridoxal phosphate-dependent enzyme -
  DK843_RS14390 (DK843_14390) pilT 2980148..2981185 (+) 1038 WP_114063686.1 type IV pilus twitching motility protein PilT Machinery gene
  DK843_RS14395 (DK843_14395) pilU 2981251..2982387 (+) 1137 WP_114061129.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DK843_RS14400 (DK843_14400) - 2982416..2984029 (+) 1614 WP_114073603.1 response regulator -
  DK843_RS14405 (DK843_14405) - 2984067..2985680 (+) 1614 WP_114073604.1 response regulator -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38287.96 Da        Isoelectric Point: 6.6642

>NTDB_id=293469 DK843_RS14390 WP_114063686.1 2980148..2981185(+) (pilT) [Chromobacterium phragmitis strain IIBBL 274-1]
MEISELLAFTVKNKASDLHLSAGLPPMIRVNGDIRRINLPPMDHHDVHDMVYDIMNDYQRKIFEDTYECDFSFELPGIAR
FRVNSFIQNRGMASVFRVIPSKVLTLEQLGAPRIFQDIANYPRGLVLVTGPTGSGKSTTLAAMIDYVNENAFSHILTVED
PIEFVHESKKSLINQRELGLQTHSFANALKSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAGEKEMVRSMLSESVRAVIAQTLLKTKDGAGRVAAHEIMIGTPAIRNLIRENKIAQINSMIQTGQQYGMQTLDQC
LADLTRRNMVSPGEARAKASNKDAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=293469 DK843_RS14390 WP_114063686.1 2980148..2981185(+) (pilT) [Chromobacterium phragmitis strain IIBBL 274-1]
ATGGAAATTTCCGAGCTCCTGGCTTTTACCGTCAAGAACAAGGCCTCCGACCTGCACTTGTCCGCCGGCCTGCCGCCGAT
GATACGGGTCAACGGCGACATCCGCCGCATCAACCTGCCGCCGATGGACCACCATGACGTGCACGACATGGTGTACGACA
TCATGAACGACTACCAGCGCAAGATTTTCGAAGACACCTACGAGTGCGATTTCTCGTTCGAGCTGCCCGGCATCGCCCGT
TTCCGCGTCAACTCCTTCATCCAGAACCGCGGCATGGCCTCGGTATTCCGGGTGATCCCGAGCAAGGTGCTGACGCTGGA
GCAACTGGGCGCGCCCCGCATCTTCCAGGACATCGCCAACTACCCCCGCGGCCTGGTGCTGGTGACCGGGCCCACCGGCT
CCGGCAAGTCCACCACGCTGGCGGCGATGATAGACTATGTCAACGAAAACGCCTTCTCGCACATTCTTACCGTGGAGGAC
CCGATCGAGTTCGTCCACGAAAGCAAGAAGAGCCTGATCAACCAGCGCGAGCTGGGCCTGCAGACCCACAGCTTCGCCAA
CGCGCTGAAAAGCGCGCTGCGCGAGGATCCGGACGTGATCCTGGTGGGCGAGTTGCGCGACCTGGAGACGATACGGCTGG
CGCTGACCGCGGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCAGCGCGGCCAAGACCATAGACCGGATC
GTCGACGTGTTCCCGGCGGGCGAGAAGGAAATGGTGCGCTCGATGTTGTCGGAATCGGTGCGCGCGGTGATCGCGCAGAC
GCTGCTGAAAACCAAGGACGGCGCCGGGCGGGTGGCGGCGCACGAAATCATGATAGGCACGCCGGCGATACGCAACCTGA
TCCGCGAGAACAAGATCGCGCAGATCAACTCGATGATACAGACCGGCCAGCAATACGGGATGCAGACGCTGGACCAGTGC
CTGGCGGACCTGACCCGGCGCAATATGGTGTCGCCGGGCGAGGCGCGGGCCAAGGCCAGCAACAAGGACGCGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A344UP30

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

72.464

100

0.725
  pilT Acinetobacter baumannii D1279779

72.464

100

0.725
  pilT Acinetobacter baumannii strain A118

72.464

100

0.725
  pilT Pseudomonas stutzeri DSM 10701

71.594

100

0.716
  pilT Neisseria gonorrhoeae MS11

71.304

100

0.713
  pilT Neisseria meningitidis 8013

71.304

100

0.713
  pilT Pseudomonas aeruginosa PAK

71.014

100

0.71
  pilT Acinetobacter baylyi ADP1

71.014

100

0.71
  pilT Legionella pneumophila strain Lp02

68.986

100

0.69
  pilT Legionella pneumophila strain ERS1305867

68.986

100

0.69
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.16

97.971

0.658
  pilT Vibrio cholerae strain A1552

67.16

97.971

0.658
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.474

95.942

0.513
  pilU Pseudomonas stutzeri DSM 10701

43.21

93.913

0.406
  pilU Vibrio cholerae strain A1552

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

40.058

99.13

0.397

Multiple sequence alignment