Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HMF8227_RS13680 Genome accession   NZ_CP029347
Coordinates   2936220..2937983 (-) Length   587 a.a.
NCBI ID   WP_109340714.1    Uniprot ID   A0A2S2E6D0
Organism   Saliniradius amylolyticus strain HMF8227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2931220..2942983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HMF8227_RS13650 (HMF8227_02744) - 2931711..2932013 (+) 303 WP_109340708.1 hypothetical protein -
  HMF8227_RS13655 (HMF8227_02745) - 2932010..2932210 (+) 201 WP_109340709.1 DUF2970 domain-containing protein -
  HMF8227_RS13660 (HMF8227_02746) - 2932126..2933025 (-) 900 WP_109340710.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  HMF8227_RS13665 (HMF8227_02747) - 2933092..2934435 (-) 1344 WP_109340711.1 SPOR domain-containing protein -
  HMF8227_RS13670 (HMF8227_02748) aroB 2934438..2935517 (-) 1080 WP_109340712.1 3-dehydroquinate synthase -
  HMF8227_RS13675 (HMF8227_02749) aroK 2935546..2936061 (-) 516 WP_109340713.1 shikimate kinase AroK -
  HMF8227_RS13680 (HMF8227_02750) pilQ 2936220..2937983 (-) 1764 WP_109340714.1 type IV pilus secretin PilQ Machinery gene
  HMF8227_RS13685 (HMF8227_02751) - 2937987..2938511 (-) 525 WP_109340715.1 pilus assembly protein PilP -
  HMF8227_RS13690 (HMF8227_02752) - 2938508..2939116 (-) 609 WP_109340716.1 type 4a pilus biogenesis protein PilO -
  HMF8227_RS13695 (HMF8227_02753) - 2939113..2939694 (-) 582 WP_109340717.1 PilN domain-containing protein -
  HMF8227_RS13700 (HMF8227_02754) pilM 2939682..2940743 (-) 1062 WP_239421317.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 587 a.a.        Molecular weight: 64064.83 Da        Isoelectric Point: 4.6156

>NTDB_id=291845 HMF8227_RS13680 WP_109340714.1 2936220..2937983(-) (pilQ) [Saliniradius amylolyticus strain HMF8227]
MSERYIFNKKLNRRHAMWPWLLLMLTCGLTARMVYAEQEVRLVDPNATNVAVQQHQLTDIEFHRSADGAAKVMVSFDKSG
VDAAVVQGEQQVEVLLPGTELAKDQLYKLDVTDFATPVTQLETFQSEEQSRILFSLSEPVTVRQQNNGSVMTLELVPKPK
EAQQQGKVSSGFEGAPISLDFQDVPVREVLQIIAQVNGFNLVTTDTVTGNVTVSLSNVPWDQALAMILKIKGLGKREEGN
ILLIAPAEELAQRETQRLESERQVQELAPLRSKTIQINYAKASDIATILKSEEGGILSERGSVTVDDRTNSVLVRDTAES
IREAEVMVKQLDIPVKQVLIESRMVTVRDNVTEELGIRWGFSDQQDSDGVSGSLEGAEAIAGGSIPSLNNRLNVNLPAAS
GNAAKIGFHIAKLADGTILDLELSALEQENKGEIIASPRITVANQHEAYIEQGTEIPYVQSTSSGATSVEFKKAVLSLKV
TPHITPDKRIILDLVVTQDTRGDTVSTSTGPAVAIDTQEIATQVLVENGETVVLGGIFQQNKTNAINKVPLFGDLPVVGG
LFRNKQDLAEKRELLIFVTPKVMTDAL

Nucleotide


Download         Length: 1764 bp        

>NTDB_id=291845 HMF8227_RS13680 WP_109340714.1 2936220..2937983(-) (pilQ) [Saliniradius amylolyticus strain HMF8227]
ATGTCCGAACGCTATATTTTCAATAAAAAACTCAATCGTCGCCATGCTATGTGGCCTTGGCTATTGCTGATGTTGACCTG
TGGACTCACGGCTCGAATGGTATATGCCGAGCAAGAAGTGCGCCTGGTGGACCCCAACGCCACAAACGTCGCTGTCCAGC
AGCATCAACTCACGGATATTGAGTTTCACCGCTCGGCGGATGGTGCGGCTAAAGTCATGGTATCGTTCGATAAATCGGGC
GTGGATGCGGCCGTGGTTCAGGGTGAACAACAGGTTGAAGTATTATTGCCGGGCACTGAACTGGCAAAAGACCAACTGTA
TAAGCTAGATGTGACGGACTTTGCGACACCAGTGACTCAGTTGGAAACCTTTCAGAGTGAGGAACAAAGCCGCATTCTGT
TTAGCTTAAGTGAGCCGGTGACGGTGCGCCAGCAAAATAACGGCTCAGTGATGACACTGGAGCTGGTGCCCAAGCCAAAA
GAGGCGCAGCAGCAGGGTAAGGTGTCTTCTGGCTTTGAGGGGGCACCCATCTCACTGGATTTTCAGGATGTGCCTGTACG
CGAAGTGCTGCAGATAATTGCCCAGGTGAACGGCTTTAATCTGGTCACCACGGACACCGTAACCGGTAATGTTACCGTAA
GCTTGTCTAATGTCCCTTGGGATCAAGCCTTGGCCATGATTCTGAAAATTAAGGGGTTAGGCAAACGCGAAGAAGGTAAT
ATCTTGCTGATTGCACCGGCTGAGGAGTTGGCCCAGCGAGAAACGCAGCGACTTGAGTCTGAGCGCCAGGTTCAGGAACT
GGCTCCGCTACGTTCTAAGACCATTCAAATTAATTACGCCAAGGCCAGTGACATCGCCACGATTCTCAAATCAGAAGAGG
GCGGCATCTTAAGCGAGCGTGGTTCGGTGACGGTGGATGACCGCACCAACTCGGTGCTGGTCAGAGATACTGCCGAGTCT
ATTCGCGAGGCGGAGGTCATGGTCAAACAACTTGATATTCCGGTCAAGCAGGTGTTGATAGAGTCTCGTATGGTCACAGT
TCGGGATAATGTCACCGAAGAGCTGGGGATTCGCTGGGGATTCAGTGATCAGCAGGACAGCGATGGCGTGTCCGGCTCAC
TGGAAGGTGCTGAAGCTATCGCCGGTGGGTCTATCCCCTCTTTGAATAATCGCTTGAATGTGAATCTGCCGGCGGCCAGT
GGCAATGCAGCCAAAATTGGATTCCATATTGCCAAGTTGGCTGACGGGACGATTTTGGATTTGGAGTTGTCCGCACTGGA
GCAGGAAAACAAAGGCGAAATCATCGCCAGCCCCCGCATTACTGTGGCTAATCAGCACGAGGCCTACATTGAGCAAGGTA
CTGAAATCCCCTATGTTCAGTCCACGTCCAGTGGCGCCACCTCGGTGGAGTTTAAAAAAGCCGTATTGAGTCTTAAGGTC
ACGCCCCACATCACGCCGGACAAACGAATCATTCTGGATTTAGTCGTGACTCAGGATACCCGTGGCGATACGGTGTCTAC
CTCCACTGGCCCGGCGGTGGCCATCGATACTCAGGAAATCGCCACTCAGGTACTGGTGGAAAATGGGGAGACCGTGGTGC
TGGGGGGGATCTTCCAGCAGAACAAGACCAATGCCATCAACAAGGTACCGTTGTTTGGTGATTTACCCGTGGTGGGGGGG
CTGTTCCGTAACAAACAGGATCTGGCGGAAAAACGCGAATTGTTGATTTTTGTCACCCCAAAAGTGATGACCGACGCCCT
CTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(425-583)

Secretin and TonB N terminus short domain (STN)

(201-246)

Bacterial type II/III secretion system short domain (Secretin_N)

(273-337)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S2E6D0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

48.881

98.978

0.484
  pilQ Vibrio cholerae strain A1552

48.881

98.978

0.484
  pilQ Vibrio campbellii strain DS40M4

48.713

92.675

0.451
  pilQ Legionella pneumophila strain Lp02

44.037

92.845

0.409
  pilQ Legionella pneumophila strain ERS1305867

43.853

92.845

0.407
  pilQ Pseudomonas aeruginosa PAK

38.462

95.23

0.366
  comQ Acinetobacter baylyi ADP1

36.301

99.489

0.361
  pilQ Acinetobacter baumannii D1279779

36.054

100

0.361
  pilQ Acinetobacter baumannii strain A118

36.054

100

0.361

Multiple sequence alignment