Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CSC28_RS26815 Genome accession   NZ_CP029093
Coordinates   5308928..5310145 (+) Length   405 a.a.
NCBI ID   WP_034081324.1    Uniprot ID   A0A2R3IUS0
Organism   Pseudomonas paraeruginosa strain AR441     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5303928..5315145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSC28_RS26800 (CSC28_5293) - 5304526..5306466 (-) 1941 WP_034081327.1 hypothetical protein -
  CSC28_RS26805 (CSC28_5294) - 5306531..5306998 (-) 468 WP_034081326.1 pilin -
  CSC28_RS26810 (CSC28_5295) pilB 5307221..5308924 (+) 1704 WP_033998207.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CSC28_RS26815 (CSC28_5296) pilC 5308928..5310145 (+) 1218 WP_034081324.1 type II secretion system F family protein Machinery gene
  CSC28_RS26820 (CSC28_5297) pilD 5310148..5311020 (+) 873 WP_058146767.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CSC28_RS26825 (CSC28_5298) coaE 5311017..5311628 (+) 612 WP_003152767.1 dephospho-CoA kinase -
  CSC28_RS26830 (CSC28_5299) yacG 5311625..5311825 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  CSC28_RS26835 (CSC28_5300) - 5311863..5312072 (-) 210 WP_033998211.1 hypothetical protein -
  CSC28_RS26840 (CSC28_5301) - 5312178..5312867 (-) 690 WP_012077298.1 energy-coupling factor ABC transporter permease -
  CSC28_RS26845 (CSC28_5302) - 5312864..5313334 (-) 471 WP_079385052.1 FAD/FMN-containing dehydrogenase -
  CSC28_RS26850 (CSC28_5303) - 5313331..5313756 (-) 426 WP_003152776.1 GNAT family N-acetyltransferase -
  CSC28_RS26855 (CSC28_5304) - 5313888..5314517 (+) 630 WP_033998217.1 DUF1780 domain-containing protein -
  CSC28_RS26860 (CSC28_5305) - 5314514..5314954 (+) 441 WP_012077299.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44159.86 Da        Isoelectric Point: 9.7208

>NTDB_id=290447 CSC28_RS26815 WP_034081324.1 5308928..5310145(+) (pilC) [Pseudomonas paraeruginosa strain AR441]
MADKALKTSVFTWEGTDKKGAKVKGELTSQNPMLVKAQLRKQGINPLKVRKKGISLTGRGKKIKPMDIALFTRQLSTMMG
AGVPLLQSFDIIGEGFDNPNMRKLVEEIKQEVASGNSLANSLRKKPMYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIRKAMTYPIAVVIVAVIVSAILLIKVVPQFQSVFEGFGAELPAFTQMVINISEFLQEWWLLALAGLIGVGFVLK
EAYKRSEKLRDATDRNLLKLPVVGNILYKSAVARYARTLSTTFAAGVPLVEALDSVAGATGNVVFRNAVSRIKQDVSTGM
QLNFSMRTTNVFPSMAIQMAAIGEESGSLDEMLGKVAGFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=290447 CSC28_RS26815 WP_034081324.1 5308928..5310145(+) (pilC) [Pseudomonas paraeruginosa strain AR441]
ATGGCGGATAAAGCGTTAAAAACCAGTGTCTTCACCTGGGAAGGCACCGACAAGAAAGGGGCCAAGGTCAAGGGGGAACT
GACCAGCCAGAACCCCATGCTGGTCAAGGCGCAACTGCGAAAGCAGGGTATCAATCCGCTCAAGGTACGCAAGAAGGGCA
TTTCCCTGACGGGCCGGGGAAAAAAGATCAAGCCCATGGACATCGCCCTGTTCACGCGTCAGTTGTCCACCATGATGGGT
GCGGGCGTCCCCCTTTTGCAATCCTTCGATATCATCGGCGAAGGCTTCGACAATCCGAACATGCGCAAACTCGTCGAGGA
GATCAAGCAGGAAGTTGCCTCAGGCAATAGCCTGGCCAACTCCCTGCGCAAAAAACCCATGTACTTCGATGACCTGTACT
GCAACCTGGTGGATGCTGGCGAGCAGTCCGGTGCGCTGGAAACGTTGCTCGATCGCGTCGCGACCTACAAGGAAAAAACC
GAATCGCTGAAAGCCAAGATTCGCAAGGCCATGACCTACCCCATCGCGGTGGTCATCGTGGCAGTGATCGTCTCGGCCAT
TCTGCTGATCAAGGTCGTGCCACAGTTCCAGTCGGTATTCGAAGGCTTTGGCGCCGAGCTGCCGGCCTTTACCCAGATGG
TCATCAATATTTCCGAGTTCCTGCAGGAATGGTGGCTGCTCGCACTGGCGGGCCTGATAGGAGTGGGCTTCGTCCTGAAA
GAGGCCTATAAGCGATCGGAGAAATTACGCGATGCCACCGATCGCAACCTGTTGAAGCTTCCGGTCGTTGGCAACATCCT
CTACAAATCCGCCGTCGCCCGCTACGCGCGCACGCTTTCGACCACTTTCGCCGCCGGTGTTCCGCTGGTAGAAGCCCTGG
ATTCCGTAGCCGGAGCTACTGGCAACGTAGTCTTCAGAAATGCCGTCAGCAGGATTAAGCAAGATGTTTCCACCGGCATG
CAGCTGAATTTCTCCATGCGCACCACCAACGTATTCCCCAGCATGGCCATCCAGATGGCTGCGATCGGTGAGGAATCAGG
CTCGCTGGATGAGATGCTCGGGAAAGTAGCCGGCTTCTATGAGGAGGAAGTCGACAATGCCGTCGACAACCTAACGACAC
TCATGGAACCAATGATCATGGCAGTCCTCGGTGTGCTGGTCGGTGGTCTGATCATCGCCATGTACCTGCCGATCTTCCAA
CTGGGTTCCGTTGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R3IUS0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.259

100

0.793
  pilC Acinetobacter baylyi ADP1

59.069

100

0.595
  pilC Acinetobacter baumannii D1279779

59.158

99.753

0.59
  pilC Legionella pneumophila strain ERS1305867

55.303

97.778

0.541
  pilG Neisseria meningitidis 44/76-A

45.074

100

0.452
  pilG Neisseria gonorrhoeae MS11

44.828

100

0.449
  pilC Vibrio cholerae strain A1552

42.963

100

0.43
  pilC Vibrio campbellii strain DS40M4

41.147

99.012

0.407
  pilC Thermus thermophilus HB27

38.404

99.012

0.38

Multiple sequence alignment