Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   DC427_RS08420 Genome accession   NZ_CP029030
Coordinates   1654746..1655057 (-) Length   103 a.a.
NCBI ID   WP_000472256.1    Uniprot ID   -
Organism   Staphylococcus aureus strain CAR     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1630942..1657087 1654746..1655057 within 0


Gene organization within MGE regions


Location: 1630942..1657087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DC427_RS08275 - 1630942..1631478 (-) 537 WP_077442703.1 IS30 family transposase -
  DC427_RS08280 - 1631459..1631962 (-) 504 WP_000746375.1 transposase -
  DC427_RS08285 - 1632071..1632427 (-) 357 WP_001255379.1 cystatin-like fold lipoprotein -
  DC427_RS08290 - 1632483..1633073 (-) 591 WP_000810443.1 hypothetical protein -
  DC427_RS08295 - 1633080..1634126 (-) 1047 WP_000247465.1 CHAP domain-containing protein -
  DC427_RS08300 - 1634116..1635963 (-) 1848 WP_000681154.1 CD3337/EF1877 family mobilome membrane protein -
  DC427_RS08305 - 1635968..1637326 (-) 1359 WP_001251211.1 FtsK/SpoIIIE domain-containing protein -
  DC427_RS08310 - 1637380..1639875 (-) 2496 WP_001049264.1 ATP-binding protein -
  DC427_RS08315 - 1639910..1640293 (-) 384 WP_000358144.1 TcpE family conjugal transfer membrane protein -
  DC427_RS08320 - 1640305..1640565 (-) 261 WP_000015639.1 TcpD family membrane protein -
  DC427_RS08325 - 1640570..1641625 (-) 1056 WP_000692003.1 conjugal transfer protein -
  DC427_RS08330 - 1641686..1642777 (-) 1092 WP_000172939.1 replication initiation factor domain-containing protein -
  DC427_RS08335 - 1642952..1643254 (-) 303 WP_000386891.1 hypothetical protein -
  DC427_RS08340 - 1643268..1643588 (-) 321 WP_000805733.1 DUF961 family protein -
  DC427_RS08345 - 1643739..1644023 (-) 285 WP_000134545.1 hypothetical protein -
  DC427_RS08350 efp 1644418..1644975 (-) 558 WP_000626504.1 elongation factor P -
  DC427_RS08355 - 1645001..1646062 (-) 1062 WP_000087107.1 M24 family metallopeptidase -
  DC427_RS08360 - 1646167..1646748 (+) 582 WP_000737686.1 hypothetical protein -
  DC427_RS08365 - 1646762..1646980 (+) 219 WP_001244298.1 SA1362 family protein -
  DC427_RS08370 - 1647044..1647874 (-) 831 WP_000141108.1 biotin/lipoate A/B protein ligase family protein -
  DC427_RS08375 - 1648032..1648418 (+) 387 WP_001276571.1 rhodanese-like domain-containing protein -
  DC427_RS08380 gcvPB 1648783..1650255 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  DC427_RS08385 gcvPA 1650248..1651594 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  DC427_RS08390 gcvT 1651614..1652705 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  DC427_RS08395 - 1652864..1653388 (-) 525 WP_001015117.1 shikimate kinase -
  DC427_RS08400 - 1653378..1653524 (-) 147 WP_001789879.1 hypothetical protein -
  DC427_RS08405 comGF 1653621..1654118 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  DC427_RS08410 comGE 1654036..1654335 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  DC427_RS08415 comGD 1654322..1654768 (-) 447 WP_001791635.1 competence type IV pilus minor pilin ComGD Machinery gene
  DC427_RS08420 comGC 1654746..1655057 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  DC427_RS08425 comGB 1655071..1656141 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  DC427_RS08430 comGA 1656113..1657087 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 103 a.a.        Molecular weight: 11315.36 Da        Isoelectric Point: 8.5268

>NTDB_id=289367 DC427_RS08420 WP_000472256.1 1654746..1655057(-) (comGC) [Staphylococcus aureus strain CAR]
MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQSTGCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFI
KEAQKTCKSGETITISNGEAVAN

Nucleotide


Download         Length: 312 bp        

>NTDB_id=289367 DC427_RS08420 WP_000472256.1 1654746..1655057(-) (comGC) [Staphylococcus aureus strain CAR]
ATGTTTAAATTTCTTAAGAAAACTCAAGCGTTTACATTGATAGAGATGCTATTAGTGTTATTAATCATCAGTTTATTATT
AATTTTAATCATTCCAAATATTGCTAAACAAACTGCTCACATACAATCAACAGGTTGTAATGCACAGGTAAAAATGGTTA
ATAGTCAAATTGAAGCGTATGCATTGAAACATAATAGAAATCCATCGTCTATTGAAGACTTAATTGCAGATGGTTTTATA
AAAGAAGCACAAAAGACATGTAAATCAGGAGAGACAATCACAATTAGTAATGGAGAAGCAGTTGCAAATTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-30)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Staphylococcus aureus MW2

100

100

1
  comGC Staphylococcus aureus N315

100

100

1
  comGC/cglC Streptococcus pneumoniae TIGR4

46.078

99.029

0.456
  comGC/cglC Streptococcus pneumoniae Rx1

46.078

99.029

0.456
  comGC/cglC Streptococcus pneumoniae D39

46.078

99.029

0.456
  comGC/cglC Streptococcus pneumoniae R6

46.078

99.029

0.456
  comGC/cglC Streptococcus mitis SK321

51.163

83.495

0.427
  comGC/cglC Streptococcus mitis NCTC 12261

51.163

83.495

0.427
  comYC Streptococcus gordonii str. Challis substr. CH1

42.857

95.146

0.408
  comYC Streptococcus suis isolate S10

50

75.728

0.379
  comGC Bacillus subtilis subsp. subtilis str. 168

41.758

88.35

0.369
  comGC Lactococcus lactis subsp. cremoris KW2

46.341

79.612

0.369

Multiple sequence alignment