Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSPA7_RS24615 Genome accession   NC_009656
Coordinates   5311215..5312432 (+) Length   405 a.a.
NCBI ID   WP_012077296.1    Uniprot ID   -
Organism   Pseudomonas paraeruginosa PA7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5306215..5317432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSPA7_RS32215 (PSPA7_5160) - 5306833..5308752 (-) 1920 WP_012077293.1 hypothetical protein -
  PSPA7_RS24605 (PSPA7_5161) - 5308815..5309285 (-) 471 WP_003152762.1 pilin -
  PSPA7_RS24610 (PSPA7_5162) pilB 5309508..5311211 (+) 1704 WP_033998207.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSPA7_RS24615 (PSPA7_5163) pilC 5311215..5312432 (+) 1218 WP_012077296.1 type II secretion system F family protein Machinery gene
  PSPA7_RS24620 (PSPA7_5164) pilD 5312435..5313307 (+) 873 WP_012077297.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PSPA7_RS24625 (PSPA7_5165) coaE 5313304..5313915 (+) 612 WP_003152767.1 dephospho-CoA kinase -
  PSPA7_RS24630 (PSPA7_5166) yacG 5313912..5314112 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PSPA7_RS24635 (PSPA7_5167) - 5314150..5314359 (-) 210 WP_003152770.1 hypothetical protein -
  PSPA7_RS24640 (PSPA7_5168) - 5314465..5315154 (-) 690 WP_012077298.1 energy-coupling factor ABC transporter permease -
  PSPA7_RS24645 (PSPA7_5169) - 5315151..5315627 (-) 477 WP_003152773.1 hypothetical protein -
  PSPA7_RS24650 (PSPA7_5170) - 5315624..5316049 (-) 426 WP_003152776.1 GNAT family N-acetyltransferase -
  PSPA7_RS24655 (PSPA7_5171) - 5316181..5316810 (+) 630 WP_003152777.1 DUF1780 domain-containing protein -
  PSPA7_RS24660 (PSPA7_5172) - 5316807..5317247 (+) 441 WP_012077299.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44189.95 Da        Isoelectric Point: 9.7208

>NTDB_id=28552 PSPA7_RS24615 WP_012077296.1 5311215..5312432(+) (pilC) [Pseudomonas paraeruginosa PA7]
MADKALKTSVFTWEGTDKKGAKVKGELTSQNPMLVKAQLRKQGINPLKVRKKGISLMGRGKKIKPMDIALFTRQLSTMMG
AGVPLLQSFDIIGEGFDNPNMRKLVEEIKQEVASGNSLANSLRKKPMYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIRKAMTYPIAVVIVAVIVSAILLIKVVPQFQSVFEGFGAELPAFTQMVINISEFLQEWWLLALAGLIGVGFVLK
EAYKRSEKLRDATDRNLLKLPVVGNILYKSAVARYARTLSTTFAAGVPLVEALDSVAGATGNVVFRNAVSRIKQDVSTGM
QLNFSMRTTNVFPSMAIQMAAIGEESGSLDEMLGKVAGFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=28552 PSPA7_RS24615 WP_012077296.1 5311215..5312432(+) (pilC) [Pseudomonas paraeruginosa PA7]
ATGGCGGATAAAGCGTTAAAAACCAGTGTCTTCACCTGGGAAGGCACCGACAAGAAAGGGGCCAAGGTCAAGGGGGAACT
GACCAGCCAGAACCCCATGCTGGTCAAGGCGCAACTGCGAAAGCAGGGTATCAATCCGCTCAAGGTACGCAAGAAGGGCA
TTTCCCTGATGGGCCGGGGAAAAAAGATCAAGCCCATGGACATCGCCCTGTTCACGCGTCAGTTGTCCACCATGATGGGT
GCGGGCGTCCCCCTTTTGCAATCCTTCGATATCATCGGCGAAGGCTTCGACAATCCGAACATGCGCAAACTCGTCGAGGA
GATCAAGCAGGAAGTTGCCTCAGGCAATAGCCTGGCCAACTCCCTGCGCAAAAAGCCCATGTACTTCGATGACCTGTACT
GCAACCTGGTGGATGCTGGCGAGCAGTCCGGTGCGCTGGAAACGTTGCTCGATCGCGTCGCGACCTACAAGGAAAAAACC
GAATCGCTGAAAGCCAAGATTCGCAAGGCCATGACCTACCCCATCGCGGTAGTCATCGTGGCAGTGATCGTCTCGGCCAT
TCTGCTGATCAAGGTCGTGCCACAGTTCCAGTCGGTATTCGAAGGCTTTGGCGCCGAGCTGCCGGCCTTTACCCAGATGG
TCATCAATATTTCCGAGTTCCTGCAGGAATGGTGGCTGCTCGCACTGGCGGGCCTGATAGGAGTGGGCTTCGTCCTGAAG
GAGGCCTATAAGCGATCGGAGAAATTACGCGATGCCACCGATCGCAACCTGTTGAAGCTTCCGGTCGTTGGCAACATCCT
CTACAAATCCGCCGTCGCCCGCTACGCGCGCACACTTTCGACCACTTTCGCCGCCGGTGTTCCGCTGGTAGAAGCCCTGG
ATTCCGTAGCTGGAGCTACTGGCAACGTAGTCTTCAGAAATGCCGTCAGCAGGATTAAGCAAGATGTTTCCACCGGCATG
CAGCTGAATTTCTCCATGCGCACCACCAACGTATTCCCCAGCATGGCCATCCAGATGGCTGCGATCGGTGAGGAATCAGG
CTCGCTGGATGAGATGCTCGGGAAAGTAGCCGGCTTCTATGAGGAGGAAGTCGACAATGCCGTCGACAACCTAACGACAC
TCATGGAACCAATGATCATGGCAGTCCTCGGTGTGCTGGTCGGTGGTCTGATCATCGCCATGTACCTGCCGATCTTCCAA
CTGGGTTCCGTTGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.259

100

0.793
  pilC Acinetobacter baylyi ADP1

58.824

100

0.593
  pilC Acinetobacter baumannii D1279779

58.663

99.753

0.585
  pilC Legionella pneumophila strain ERS1305867

55.303

97.778

0.541
  pilG Neisseria meningitidis 44/76-A

45.074

100

0.452
  pilG Neisseria gonorrhoeae MS11

44.828

100

0.449
  pilC Vibrio cholerae strain A1552

42.963

100

0.43
  pilC Vibrio campbellii strain DS40M4

40.898

99.012

0.405
  pilC Thermus thermophilus HB27

38.404

99.012

0.38

Multiple sequence alignment