Detailed information NTG0028553

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PSPA7_RS24620
• Genome accession: NC_009656
• Coordinates: 5312435..5313307 (+)
• Length: 290 a.a.
• NCBI ID: WP_012077297.1
• Uniprot ID: A6VBR4
• Organism: Pseudomonas paraeruginosa PA7
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5307435..5318307

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSPA7_RS24605 (PSPA7_5161)	-	5308815..5309285 (-)	471	WP_003152762.1	pilin	-
PSPA7_RS24610 (PSPA7_5162)	pilB	5309508..5311211 (+)	1704	WP_033998207.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PSPA7_RS24615 (PSPA7_5163)	pilC	5311215..5312432 (+)	1218	WP_012077296.1	type II secretion system F family protein	Machinery gene
PSPA7_RS24620 (PSPA7_5164)	pilD	5312435..5313307 (+)	873	WP_012077297.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
PSPA7_RS24625 (PSPA7_5165)	coaE	5313304..5313915 (+)	612	WP_003152767.1	dephospho-CoA kinase	-
PSPA7_RS24630 (PSPA7_5166)	yacG	5313912..5314112 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
PSPA7_RS24635 (PSPA7_5167)	-	5314150..5314359 (-)	210	WP_003152770.1	hypothetical protein	-
PSPA7_RS24640 (PSPA7_5168)	-	5314465..5315154 (-)	690	WP_012077298.1	energy-coupling factor ABC transporter permease	-
PSPA7_RS24645 (PSPA7_5169)	-	5315151..5315627 (-)	477	WP_003152773.1	hypothetical protein	-
PSPA7_RS24650 (PSPA7_5170)	-	5315624..5316049 (-)	426	WP_003152776.1	GNAT family N-acetyltransferase	-
PSPA7_RS24655 (PSPA7_5171)	-	5316181..5316810 (+)	630	WP_003152777.1	DUF1780 domain-containing protein	-
PSPA7_RS24660 (PSPA7_5172)	-	5316807..5317247 (+)	441	WP_012077299.1	MOSC domain-containing protein	-
PSPA7_RS24665 (PSPA7_5173)	-	5317282..5317452 (+)	171	WP_003152781.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31717.72 Da
• Isoelectric Point: 7.3859

>NTDB_id=28553 PSPA7_RS24620 WP_012077297.1 5312435..5313307(+) (pilD) [Pseudomonas paraeruginosa PA7]
MPLLDYLASHPLAFVLCAIVLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLQPEPDPAIYNLVLPNSSCPHCGHEIR
PWENIPVLSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSLVG
AILGVIMLRLRNAASGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=28553 PSPA7_RS24620 WP_012077297.1 5312435..5313307(+) (pilD) [Pseudomonas paraeruginosa PA7]
ATGCCCCTTCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCGTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCACCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAGGCGCTCGGGC
TGCAGCCGGAGCCTGATCCGGCGATCTACAACCTGGTCCTGCCGAACTCCTCGTGCCCGCATTGCGGCCACGAGATCCGT
CCCTGGGAAAACATCCCGGTGCTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAGCTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGACGCGCTGTTCGGCGCGGT
GTTCGGCTACCTCAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGCGCCTGGGGCGGCTGGCAGGTCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCCATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGCAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCATTGCTCTGGGGTGATCAAATAACCCGGACCTATTTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A6VBR4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.714	96.552	0.538
pilD	Vibrio campbellii strain DS40M4	53.357	97.586	0.521
pilD	Acinetobacter baumannii D1279779	51.237	97.586	0.5
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	50.566	91.379	0.462