Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C4K03_RS04615 Genome accession   NZ_CP027754
Coordinates   1001995..1003203 (-) Length   402 a.a.
NCBI ID   WP_124376301.1    Uniprot ID   A0A3G7U171
Organism   Pseudomonas synxantha strain 30B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 996995..1008203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K03_RS04580 (C4K03_0898) - 998188..998361 (-) 174 WP_046070858.1 DUF3094 family protein -
  C4K03_RS04585 (C4K03_0899) - 998450..999079 (-) 630 WP_043049309.1 DUF1780 domain-containing protein -
  C4K03_RS04590 (C4K03_0900) - 999349..1000038 (+) 690 WP_124376298.1 energy-coupling factor ABC transporter permease -
  C4K03_RS04595 (C4K03_0901) - 1000103..1000318 (+) 216 WP_003171680.1 hypothetical protein -
  C4K03_RS04600 (C4K03_0902) yacG 1000315..1000515 (-) 201 WP_043049311.1 DNA gyrase inhibitor YacG -
  C4K03_RS04605 (C4K03_0903) coaE 1000512..1001135 (-) 624 WP_124376299.1 dephospho-CoA kinase -
  C4K03_RS04610 (C4K03_0904) pilD 1001132..1001998 (-) 867 WP_124376300.1 A24 family peptidase Machinery gene
  C4K03_RS04615 (C4K03_0905) pilC 1001995..1003203 (-) 1209 WP_124376301.1 type II secretion system F family protein Machinery gene
  C4K03_RS04620 (C4K03_0906) - 1003405..1003818 (+) 414 WP_057013848.1 pilin -
  C4K03_RS04630 (C4K03_0908) - 1004106..1004411 (+) 306 WP_124376302.1 DUF6388 family protein -
  C4K03_RS04635 (C4K03_0909) nadC 1004531..1005379 (-) 849 WP_124376303.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C4K03_RS04640 (C4K03_0910) - 1005625..1007778 (+) 2154 WP_124376304.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43518.08 Da        Isoelectric Point: 10.1965

>NTDB_id=280116 C4K03_RS04615 WP_124376301.1 1001995..1003203(-) (pilC) [Pseudomonas synxantha strain 30B]
MSDTSTIYAWEGINRKGRRVSGQIAGHDLALVKAQLRRKGICPERVRKKPTSRPIFAPHILPADIALFTRQLATLLKAGI
PLLQAFDITSEGVENRQVCDLVKALKQQIASGNSLADALRQHPRYFDDLYCNLVAAGEQAGALETLLERVAIHLEKSQRL
KARIKKAMTYPVTVLVVATLVSVVLLMHVVPQFQKLFTGVGGQLPGFTLAVIALSEFLQQAWWMLALGLGASLAGLRQAY
RAYPGFRYRLQAGLLKVPLAGKLLHNSAVARYARTLSTTFAAGVPLVQALGSVAGAVGNGPFKQAIERMRHDVSTGMQLN
QAMASNGLFPGMAIQMTAIGEESGTLDRMLEKVANHYEADVDTLVDNLTSLMEPLIMVLLGGIVGALVIALYLPVFQLGT
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=280116 C4K03_RS04615 WP_124376301.1 1001995..1003203(-) (pilC) [Pseudomonas synxantha strain 30B]
ATGAGCGACACTTCAACGATCTACGCCTGGGAAGGCATCAACCGCAAGGGACGCAGGGTGTCCGGGCAAATTGCTGGCCA
TGACCTTGCGCTGGTCAAGGCACAATTACGCCGCAAAGGGATCTGCCCCGAGCGTGTGCGCAAGAAGCCTACATCACGGC
CGATCTTTGCCCCACACATATTGCCTGCGGATATCGCCCTGTTCACCCGCCAGTTGGCCACATTGCTCAAGGCCGGCATC
CCGTTGCTACAAGCCTTCGACATCACCAGCGAGGGCGTCGAGAACCGGCAGGTGTGTGACCTGGTCAAAGCCTTGAAACA
GCAGATCGCCAGCGGCAACAGCTTGGCCGATGCGCTGCGCCAACATCCACGTTATTTCGACGATCTGTACTGCAACCTGG
TCGCCGCCGGGGAACAGGCAGGCGCCCTGGAAACCTTACTGGAGCGCGTGGCGATTCACCTGGAAAAAAGCCAACGGCTC
AAAGCCAGGATCAAAAAGGCCATGACCTACCCTGTCACGGTATTGGTGGTCGCCACACTGGTCAGCGTGGTGTTACTGAT
GCACGTCGTGCCGCAATTCCAGAAACTGTTCACCGGCGTCGGCGGCCAATTGCCCGGGTTTACCTTGGCGGTCATTGCCC
TGTCCGAATTCCTGCAACAGGCGTGGTGGATGCTGGCGCTGGGCTTGGGTGCGTCGCTGGCAGGGCTTCGCCAGGCTTAC
CGTGCCTACCCTGGCTTTCGCTATCGGCTGCAAGCGGGTTTGTTGAAAGTGCCCCTGGCAGGCAAACTGCTGCACAACTC
TGCCGTCGCTCGTTACGCGCGTACGCTTTCGACCACGTTTGCAGCGGGCGTGCCCCTCGTGCAGGCATTGGGCTCGGTGG
CCGGCGCCGTCGGCAATGGACCATTCAAACAGGCAATTGAACGAATGCGCCACGATGTATCCACAGGCATGCAATTGAAC
CAGGCCATGGCCAGCAATGGCCTGTTTCCAGGCATGGCGATCCAGATGACGGCCATCGGTGAAGAGTCCGGCACGCTGGA
CCGAATGTTGGAAAAAGTCGCGAACCACTACGAGGCGGACGTGGACACCTTGGTCGATAACCTCACCAGCCTGATGGAAC
CCCTGATCATGGTGCTACTGGGGGGCATTGTCGGCGCGTTGGTGATCGCCCTGTACCTGCCGGTCTTTCAATTGGGTACG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G7U171

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.784

99.005

0.562
  pilC Acinetobacter baylyi ADP1

51.01

98.507

0.502
  pilC Acinetobacter baumannii D1279779

48.861

98.259

0.48
  pilC Legionella pneumophila strain ERS1305867

48.111

98.756

0.475
  pilC Thermus thermophilus HB27

38.213

100

0.383
  pilG Neisseria gonorrhoeae MS11

40.157

94.776

0.381
  pilC Vibrio cholerae strain A1552

38.325

98.01

0.376
  pilG Neisseria meningitidis 44/76-A

39.37

94.776

0.373
  pilC Vibrio campbellii strain DS40M4

38.01

97.512

0.371

Multiple sequence alignment